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[182309_s_at]

Description F53H8.4 /REP_DB=WormBase Gene ID /WP=CE04657 /TR=Q20735 /GB=AAA82341.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 18      4grp_high_8_8 > 35      6grp_total > 17      4grp_total > 45      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 147
1 2 3 4 range
A 208.4
(1.6)
P 		208.5
(1.6)
P 		213.6
(3)
P 		191.9
(-3)
P 		22
B 273.3
(3)
P 		240.2
(1.6)
P 		135.7
(-3)
P 		144
(-2.6)
P 		138
C 255.5
(3)
P 		166.1
(-1.2)
A 		156.3
(-1.6)
P 		126.5
(-3)
P 		129
avr 245.7
(3) 		204.9
(0.3) 		168.5
(-2.1) 		154.1
(-3) 		92

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 289
1 2 3 4 5 6 range
A 390.9
(3)
P 		238.4
(-0.2)
P 		101.9
(-3)
P 		125.8
(-2.5)
P 		195.5
(-1.1)
A 		168.2
(-1.6)
P 		289
B 268.4
(3)
P 		224.4
(0.9)
P 		192.6
(-0.6)
P 		205.7
(0)
P 		186.1
(-0.9)
P 		142.6
(-3)
P 		126
C 268.6
(3)
P 		206.7
(0.3)
P 		170.5
(-1.2)
P 		128.5
(-3)
A 		156.1
(-1.8)
A 		158.2
(-1.7)
A 		140
avr 309.3
(3) 		223.2
(-0.3) 		155
(-2.9) 		153.3
(-3) 		179.2
(-2) 		156.3
(-2.9) 		156
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 75.9
(3)
P 		45.3
(-0.8)
P 		55.8
(0.5)
P 		28.1
(-3)
P 		48
B 87.1
(3)
P 		59.3
(0)
P 		31.4
(-2.9)
P 		30.7
(-3)
P 		56
C 84.6
(3)
P 		47.7
(-0.5)
A 		54.5
(0.1)
P 		21.3
(-3)
P 		63
avr 82.5
(3) 		50.8
(-0.4) 		47.2
(-0.8) 		26.7
(-3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 72.1
(1.8)
P 		84.8
(3)
P 		22.2
(-3)
P 		31.9
(-2.1)
P 		59
(0.5)
A 		41
(-1.2)
P 		63
B 93.4
(3)
P 		74.7
(0.6)
P 		46.8
(-3)
P 		54.5
(-2)
P 		75.6
(0.7)
P 		53.2
(-2.2)
P 		47
C 99.6
(3)
P 		63.1
(0)
P 		43.4
(-1.6)
P 		25.8
(-3)
A 		50.5
(-1)
A 		53
(-0.8)
A 		74
avr 88.4
(3) 		74.2
(1.3) 		37.5
(-3) 		37.4
(-3) 		61.7
(-0.1) 		49.1
(-1.6) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182309_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18559
Sequence Type Exemplar sequence
Representative Public ID F53H8.4 NCBI
Target Description F53H8.4 /REP_DB=WormBase Gene ID /WP=CE04657 /TR=Q20735 /GB=AAA82341.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075781(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075781 NCBI Caenorhabditis elegans SphingoMyelin Synthase family member (sms-2) (sms-2) mRNA, complete cds. 11/11 None
GENEFINDER00000026710 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:945265:948880:-1 11/11 None
F53H8.4 ENSEMBL cdna:known chromosome:CEL140:X:944827:948882:-1 gene:F53H8.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026700 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:945264-948880(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sms-2
Ensembl F53H8.4
Entrez Gene 180445 Entrez gene
SwissProt Q20735 EMBL-EBI
WormBase CE04657 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA82341 Sphingomyelin synthase protein 2 [Caenorhabditis elegans] ref|NP_508182.1| SphingoMyelin Synthase family member (sms-2) [Caenorhabditis elegans] sp|Q20735|SMS2_CAEEL Putative phosphatidylcholine:ceramide cholinephosphotransferase 2 (Sphingomyelin synthase 2) 0.0
blast CAE63595 Hypothetical protein CBG08086 [Caenorhabditis briggsae] 0.0
blast XP_392299 PREDICTED: similar to ENSANGP00000021414 [Apis mellifera] 9.0E-42
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508182.1 6 60-82,102-124,136-155,200-222,229-249,259-276
F53H8.4 6 60-82,102-124,136-155,200-222,229-249,259-276
GENEFINDER00000026710 6 60-82,102-124,136-155,200-222,229-249,259-276
Sequence
Target Sequence 
>C. ELEGANS:182309_S_AT
ttgggagctatcatgtacggcctgcgtgccgtgattctcggtgtcactttcctgccgccc
tcttttcacaatagggatgaaatttgccagccacaagtcaacagaaccgctatgtacggc
atggaaatcgcgacgagattcctcacctacgtcatcactttgggcttgacatccggacaa
gacaagattctgtgcggtgatctgatgtttagcgggcacactgtcgttctcacaattatg
tattttgtgcagttgcagtacactccgcgtggtctcgttattctcagatacattgccgct
ccgatcacattcctcggaatcgcagctctcgtcgtgtcaggcggtcactatacgatggac
gtgttaattgcgtactggttgacaagtcacgttttctggtcttatcaccaaattttcgaa
atgcgaaaggacgatcgaccacaggcgccgttgagtcgactttggtggttctggttgtgc
tactggtttgaatcggatgttgcggacgggaaattggtgaacaagtggaattggccgctg
gaaggccctcaaagaatgcacacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGGAGCTATCATGTACGGCCTGC 169 707 424 Antisense
TGCCGCCCTCTTTTCACAATAGGGA 674 583 476 Antisense
GCCAGCCACAAGTCAACAGAACCGC 658 279 509 Antisense
GGAAATCGCGACGAGATTCCTCACC 538 527 546 Antisense
GCACACTGTCGTTCTCACAATTATG 445 317 639 Antisense
GTTATTCTCAGATACATTGCCGCTC 642 447 700 Antisense
ATCACATTCCTCGGAATCGCAGCTC 592 27 727 Antisense
AAGTCACGTTTTCTGGTCTTATCAC 172 157 807 Antisense
TCGACCACAGGCGCCGTTGAGTCGA 531 597 858 Antisense
TGAGTCGACTTTGGTGGTTCTGGTT 515 563 875 Antisense
GGAAGGCCCTCAAAGAATGCACACC 182 533 963 Antisense
Sequence Source Affymetrix Proprietary Database