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[182306_at]

Description K05F1.4 /REP_DB=WormBase Gene ID /WP=CE02808 /TR=Q21242 /GB=AAA68719.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 55      6grp_high1_10_10 > 44      4grp_high_8_8 > 52      6grp_highest_nonorm > 72      6grp_total > 0      4grp_total > 59      6grp_total_sub > Hat      6grp_high1_sub > 02      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 405
1 2 3 4 range
A 297.2
(-0.2)
P 		512.3
(3)
P 		478.7
(2.5)
P 		107
(-3)
A 		405
B 352.7
(0.5)
P 		511.4
(3)
P 		437
(1.8)
P 		125.8
(-3)
P 		386
C 370
(0.9)
P 		432.8
(1.9)
P 		496.3
(3)
P 		140
(-3)
A 		356
avr 340
(0.6) 		485.5
(3) 		470.7
(2.8) 		124.3
(-3) 		361

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 410
1 2 3 4 5 6 range
A 195.6
(-3)
P 		491.9
(2.8)
P 		499.5
(3)
P 		460.5
(2.2)
P 		374.6
(0.5)
P 		441.9
(1.9)
P 		304
B 350.2
(-3)
P 		605.1
(3)
P 		403.7
(-1.7)
P 		370.1
(-2.5)
P 		473.9
(-0.1)
P 		546.7
(1.6)
P 		255
C 337.2
(-3)
P 		601.3
(3)
P 		430.6
(-0.9)
P 		356
(-2.6)
P 		367.3
(-2.3)
P 		544.5
(1.7)
P 		264
avr 294.3
(-3) 		566.1
(3) 		444.6
(0.3) 		395.5
(-0.8) 		405.3
(-0.5) 		511
(1.8) 		272
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 108.2
(2.1)
P 		111.2
(2.2)
P 		125.1
(3)
P 		15.7
(-3)
A 		109
B 112.5
(2.2)
P 		126.3
(3)
P 		101.3
(1.5)
P 		26.8
(-3)
P 		100
C 122.5
(1)
P 		124.4
(1)
P 		173
(3)
P 		23.6
(-3)
A 		149
avr 114.4
(2) 		120.6
(2.3) 		133.1
(3) 		22
(-3) 		111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 36.1
(-3)
P 		174.9
(3)
P 		108.8
(0.1)
P 		116.8
(0.5)
P 		113
(0.3)
P 		107.6
(0.1)
P 		139
B 121.9
(-1.6)
P 		201.4
(2.9)
P 		98
(-3)
P 		98
(-3)
P 		192.5
(2.4)
P 		203.8
(3)
P 		106
C 125
(-0.1)
P 		183.5
(3)
P 		109.6
(-1)
P 		71.6
(-3)
P 		118.8
(-0.5)
P 		182.3
(2.9)
P 		112
avr 94.3
(-3) 		186.6
(3) 		105.5
(-2.3) 		95.5
(-2.9) 		141.4
(0.1) 		164.6
(1.6) 		92
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182306_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5002
Sequence Type Exemplar sequence
Representative Public ID K05F1.4 NCBI
Target Description K05F1.4 /REP_DB=WormBase Gene ID /WP=CE02808 /TR=Q21242 /GB=AAA68719.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062740(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062740 NCBI Caenorhabditis elegans K05F1.5 (K05F1.5) mRNA, complete cds. 11/11 A 
SNAP00000031242 ENSEMBL cdna:SNAP chromosome:CEL140:II:5792728:5794240:1 11/11 None
GENEFINDER00000031251 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5790702:5794240:1 11/11 None
K05F1.5 ENSEMBL cdna:known chromosome:CEL140:II:5789137:5794357:1 gene:K05F1.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5792337-5794188(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K05F1.5
Entrez Gene 173978 Entrez gene
SwissProt Q21241 EMBL-EBI
WormBase CE28587 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68720 Hypothetical protein K05F1.5 [Caenorhabditis elegans] ref|NP_495141.1| K05F1.5 [Caenorhabditis elegans] 0.0
blast CAE59189 Hypothetical protein CBG02500 [Caenorhabditis briggsae] 0.0
blast CAE59189 Hypothetical protein CBG02500 [Caenorhabditis briggsae] 1.0E-173
blast AAA68720 Hypothetical protein K05F1.5 [Caenorhabditis elegans] ref|NP_495141.1| K05F1.5 [Caenorhabditis elegans] 1.0E-170
blast CAE59189 Hypothetical protein CBG02500 [Caenorhabditis briggsae] 1.0E-158
blast T16567 hypothetical protein K05F1.4 - Caenorhabditis elegans 1.0E-150
Protein Domains
Database ID Description E-Value
Pfam IPR008663 EMBL-EBI Leukocyte cell-derived chemotaxin 2 7.8E-120
Pfam IPR008663 EMBL-EBI Leukocyte cell-derived chemotaxin 2 8.1E-81
Pfam IPR008663 EMBL-EBI Leukocyte cell-derived chemotaxin 2 2.8E-29
Pfam IPR008663 EMBL-EBI Leukocyte cell-derived chemotaxin 2 9.3E-80
Sequence
Target Sequence 
>C. ELEGANS:182306_AT
agggctacgcagtgcacatatcttctgttaaattgtcctttttcggagggcatgttgaag
ctggagatgagattggagaagctttgaatcgatattgtttcaatgacagaggacaaaatg
atgtcgagcctcatgttgagattcggttgtacaaagaaggaagacttatcgatccgactc
atcatcttcaaaattgcatgtgtactggtcagatctgtgaatcaaacactagaaatgtgc
tccttggtgacccttttaaaactgacaaacgttacaacggagttcgtggttgggatgttg
aatgtcaaatgattgatgatgatgatgaagattcaccacgtgcaccaatgatctattcac
caattgctggagaaatcgttggaagaattcgtcttttcactgactcaaatggagcttaca
ccggatgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGGGCTACGCAGTGCACATATCTTC 17 59 308 Antisense
CATATCTTCTGTTAAATTGTCCTTT 305 215 324 Antisense
AATTGTCCTTTTTCGGAGGGCATGT 149 173 338 Antisense
GTCGAGCCTCATGTTGAGATTCGGT 111 475 430 Antisense
GACTTATCGATCCGACTCATCATCT 384 371 470 Antisense
AATTGCATGTGTACTGGTCAGATCT 541 177 499 Antisense
TAGAAATGTGCTCCTTGGTGACCCT 478 639 537 Antisense
GATTCACCACGTGCACCAATGATCT 205 431 637 Antisense
AATGATCTATTCACCAATTGCTGGA 490 163 654 Antisense
GAATTCGTCTTTTCACTGACTCAAA 162 325 692 Antisense
TCAAATGGAGCTTACACCGGATGTG 204 629 712 Antisense
Sequence Source Affymetrix Proprietary Database