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[182303_at]

Description F28H1.4 /REP_DB=WormBase Gene ID /WP=CE09769 /TR=O01539 /GB=AAB52340.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 10      6grp_highest_nonorm > 71      6grp_total > 09      4grp_total > 50      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 328
1 2 3 4 range
A 171.3
(-1.6)
P 		406.6
(3)
P 		209.9
(-0.9)
P 		100.1
(-3)
A 		307
B 185.1
(0.2)
P 		280.4
(3)
P 		257.8
(2.3)
P 		79.1
(-3)
A 		201
C 134.2
(-3)
A 		310.4
(3)
P 		230.7
(0.3)
P 		137.7
(-2.9)
A 		176
avr 163.5
(-1.5) 		332.5
(3) 		232.8
(0.4) 		105.6
(-3) 		227

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 325
1 2 3 4 5 6 range
A 499.5
(3)
P 		455.1
(2.2)
P 		436.8
(1.8)
P 		385
(0.9)
P 		328.5
(-0.2)
P 		174.7
(-3)
P 		325
B 276.9
(1.9)
P 		190
(-3)
P 		237.3
(-0.3)
P 		296.7
(3)
P 		283.6
(2.3)
P 		259.3
(0.9)
P 		107
C 472.6
(3)
P 		321.6
(-0)
P 		336.8
(0.3)
P 		431.7
(2.2)
P 		409.8
(1.7)
P 		174.8
(-3)
P 		298
avr 416.3
(3) 		322.2
(0.4) 		337
(0.8) 		371.1
(1.7) 		340.6
(0.9) 		202.9
(-3) 		213
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 62.4
(0.9)
P 		88.3
(3)
P 		54.9
(0.3)
P 		14.6
(-3)
A 		74
B 59
(1.8)
P 		69.3
(3)
P 		59.7
(1.9)
P 		16.9
(-3)
A 		52
C 44.4
(-1.1)
A 		89.3
(3)
P 		80.4
(2.2)
P 		23.2
(-3)
A 		66
avr 55.3
(0.5) 		82.3
(3) 		65
(1.4) 		18.2
(-3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 92.1
(-0.5)
P 		161.9
(3)
P 		95.1
(-0.4)
P 		97.6
(-0.2)
P 		99.1
(-0.2)
P 		42.5
(-3)
P 		119
B 96.4
(1)
P 		63.3
(-2.4)
P 		57.6
(-3)
P 		78.6
(-0.8)
P 		115.2
(3)
P 		96.7
(1.1)
P 		58
C 175.2
(3)
P 		98.1
(-1)
P 		85.7
(-1.6)
P 		86.8
(-1.5)
P 		132.6
(0.8)
P 		58.5
(-3)
P 		117
avr 121.2
(3) 		107.8
(1.5) 		79.5
(-1.5) 		87.7
(-0.6) 		115.6
(2.4) 		65.9
(-3) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182303_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.193
Sequence Type Exemplar sequence
Representative Public ID F28H1.4 NCBI
Target Description F28H1.4 /REP_DB=WormBase Gene ID /WP=CE09769 /TR=O01539 /GB=AAB52340.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026255(11), NM_001026256(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026255 NCBI Caenorhabditis elegans F28H1.4a (F28H1.4) mRNA, complete cds. 11/11 None
NM_001026256 NCBI Caenorhabditis elegans F28H1.4b (F28H1.4) mRNA, complete cds. 11/11 None
SNAP00000015484 ENSEMBL cdna:SNAP chromosome:CEL140:I:3974129:3977220:-1 11/11 None
GENEFINDER00000015493 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3974129:3979511:-1 11/11 None
F28H1.4a ENSEMBL cdna:known chromosome:CEL140:I:3974129:3977220:-1 gene:F28H1.4 11/11 None
F28H1.4b ENSEMBL cdna:known chromosome:CEL140:I:3974129:3976773:-1 gene:F28H1.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000030466 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3964319-3965316(+) 61.82 61.82
chrI:3974128-3977220(-) 100.0 100.0
Public Domain and Genome References
Ensembl F28H1.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001021426 F28H1.4a [Caenorhabditis elegans] gb|AAB52340.2| Hypothetical protein F28H1.4a [Caenorhabditis elegans] 1.0E-112
blast NP_001021426 F28H1.4a [Caenorhabditis elegans] gb|AAB52340.2| Hypothetical protein F28H1.4a [Caenorhabditis elegans] 1.0E-110
blast CAE66737 Hypothetical protein CBG12087 [Caenorhabditis briggsae] 8.0E-95
blast CAE66737 Hypothetical protein CBG12087 [Caenorhabditis briggsae] 2.0E-94
blast NP_001021427 F28H1.4b [Caenorhabditis elegans] gb|AAV58868.1| Hypothetical protein F28H1.4b [Caenorhabditis elegans] 6.0E-88
blast NP_001021426 F28H1.4a [Caenorhabditis elegans] gb|AAB52340.2| Hypothetical protein F28H1.4a [Caenorhabditis elegans] 6.0E-88
Protein Domains
Database ID Description E-Value
Pfam IPR008253 EMBL-EBI Marvel 1.9E-33
Pfam IPR008253 EMBL-EBI Marvel 1.9E-33
Pfam IPR008253 EMBL-EBI Marvel 1.9E-33
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021426.1 4 128-150,155-177,190-212,217-236
NP_001021427.1 4 48-70,75-97,110-132,137-156
F28H1.4a 4 128-150,155-177,190-212,217-236
F28H1.4b 4 48-70,75-97,110-132,137-156
SNAP00000015484 4 128-150,155-177,190-212,217-236
GENEFINDER00000015493 4 242-264,269-291,304-326,331-350
Sequence
Target Sequence 
>C. ELEGANS:182303_AT
ggagaaaatgtatgattcaccgaggtatcagacaactacaacagttgtagaaacgacgga
atatattgggaatgatgggcctgctgttaggatcgagttccctcgcctcgactgtgaata
catcagaacattgggtggaatcatgaagatcgtctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGAAAATGTATGATTCACCGAGG 407 517 246 Antisense
GATTCACCGAGGTATCAGACAACTA 233 431 259 Antisense
ACCGAGGTATCAGACAACTACAACA 556 85 264 Antisense
ACAACAGTTGTAGAAACGACGGAAT 462 1 283 Antisense
GAAACGACGGAATATATTGGGAATG 18 359 295 Antisense
ATTGGGAATGATGGGCCTGCTGTTA 513 3 310 Antisense
GATGGGCCTGCTGTTAGGATCGAGT 358 405 319 Antisense
GCTGTTAGGATCGAGTTCCCTCGCC 128 291 328 Antisense
CCTCGCCTCGACTGTGAATACATCA 81 267 346 Antisense
GAATACATCAGAACATTGGGTGGAA 560 325 361 Antisense
GGGTGGAATCATGAAGATCGTCTGC 256 497 378 Antisense
Sequence Source Affymetrix Proprietary Database