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[182301_at]

Description H12D21.9 /REP_DB=WormBase Gene ID /WP=CE20959 /TR=Q9XTW6 /GB=CAB07432.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 10      6grp_total > 31      4grp_total > 51      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 152.1
(-1.1)
P 		242.9
(3)
P 		156.4
(-0.9)
P 		109.2
(-3)
A 		134
B 156.6
(1.7)
P 		171.1
(2.5)
P 		180.6
(3)
P 		73.6
(-3)
M 		107
C 167.3
(-0.7)
P 		220.4
(3)
P 		202.7
(1.8)
P 		134.3
(-3)
A 		86
avr 158.7
(0) 		211.5
(3) 		179.9
(1.2) 		105.7
(-3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 172
1 2 3 4 5 6 range
A 283.4
(3)
P 		208.9
(0.4)
P 		245.1
(1.7)
P 		220.3
(0.8)
P 		163.3
(-1.2)
P 		111.7
(-3)
A 		172
B 262.7
(3)
P 		207.5
(0.7)
P 		238
(2)
P 		262.2
(3)
P 		216.8
(1.1)
P 		116.2
(-3)
P 		147
C 182.2
(-0.5)
P 		235.7
(2)
P 		205.3
(0.6)
P 		257.9
(3)
P 		208.6
(0.7)
P 		126.5
(-3)
P 		131
avr 242.8
(2.8) 		217.4
(1.6) 		229.5
(2.2) 		246.8
(3) 		196.2
(0.6) 		118.1
(-3) 		129
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 55.4
(3)
P 		52.7
(2.6)
P 		40.9
(0.8)
P 		16
(-3)
A 		39
B 49.9
(3)
P 		42.3
(1.7)
P 		41.8
(1.6)
P 		15.7
(-3)
M 		34
C 55.4
(1.1)
P 		63.4
(2.1)
P 		70.6
(3)
P 		22.7
(-3)
A 		48
avr 53.6
(3) 		52.8
(2.9) 		51.1
(2.6) 		18.1
(-3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 52.2
(0.2)
P 		74.3
(3)
P 		53.4
(0.3)
P 		55.9
(0.7)
P 		49.3
(-0.2)
P 		27.2
(-3)
A 		47
B 91.4
(3)
P 		69.1
(0.2)
P 		57.8
(-1.2)
P 		69.4
(0.3)
P 		88.1
(2.6)
P 		43.3
(-3)
P 		48
C 67.5
(2.1)
P 		71.9
(3)
P 		52.3
(-1)
P 		51.9
(-1.1)
P 		67.5
(2.1)
P 		42.3
(-3)
P 		30
avr 70.4
(2.8) 		71.8
(3) 		54.5
(-0) 		59.1
(0.8) 		68.3
(2.4) 		37.6
(-3) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182301_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14678
Sequence Type Exemplar sequence
Representative Public ID H12D21.9 NCBI
Target Description H12D21.9 /REP_DB=WormBase Gene ID /WP=CE20959 /TR=Q9XTW6 /GB=CAB07432.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074275(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074275 NCBI Caenorhabditis elegans H12D21.9 (H12D21.9) mRNA, complete cds. 11/11 None
SNAP00000026970 ENSEMBL cdna:SNAP chromosome:CEL140:V:14910136:14911708:-1 11/11 None
GENEFINDER00000026974 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14910154:14911708:-1 11/11 None
H12D21.9 ENSEMBL cdna:known chromosome:CEL140:V:14910031:14911708:-1 gene:H12D21.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14910030-14911708(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H12D21.9
Entrez Gene 186731 Entrez gene
SwissProt Q9XTW6 EMBL-EBI
WormBase CE20959 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07432 Hypothetical protein H12D21.9 [Caenorhabditis elegans] ref|NP_506676.1| H12D21.9 [Caenorhabditis elegans] 1.0E-135
blast CAB07432 Hypothetical protein H12D21.9 [Caenorhabditis elegans] ref|NP_506676.1| H12D21.9 [Caenorhabditis elegans] 1.0E-126
blast CAB07432 Hypothetical protein H12D21.9 [Caenorhabditis elegans] ref|NP_506676.1| H12D21.9 [Caenorhabditis elegans] 1.0E-117
blast CAE66356 Hypothetical protein CBG11611 [Caenorhabditis briggsae] 1.0E-113
blast CAE66356 Hypothetical protein CBG11611 [Caenorhabditis briggsae] 1.0E-112
blast CAE66356 Hypothetical protein CBG11611 [Caenorhabditis briggsae] 1.0E-103
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506676.1 4 102-124,129-151,158-180,208-230
H12D21.9 4 102-124,129-151,158-180,208-230
SNAP00000026970 4 91-113,118-140,147-169,197-219
GENEFINDER00000026974 4 102-124,129-151,158-180,208-230
Sequence
Target Sequence 
>C. ELEGANS:182301_AT
gtcacatcaaaaattctcaccccaaatccaaggactattttaatttgtgttctgattgca
gcagccagtgctattcaattactaatattctcaatgattgctgtattctacgatggacca
ttcccctggtcactgatactctgtattctgatgttatgtaattgtctagttgtattcata
tttattagatgtcaacctacaagaggttggctgatatccagtctgatagctgctgcattg
tcatttatcgaatgtacggcgctatttttctggacagcattgcttatcagtcaagaagaa
aagtatctggaaagtgtaggaatacacgataatcggatagtgacaagtacaagaatagca
atgtacatgtgtcaaatgttatttgggcccatacatttaatagcatgtggccttattatt
tggaagctacgaacacagctatcaccatctgatgaggtggttaatgggtatttcttgagt
gaaccagaacttggtcaccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCACATCAAAAATTCTCACCCCAA 459 465 280 Antisense
TTCTCACCCCAAATCCAAGGACTAT 107 691 293 Antisense
GATTGCAGCAGCCAGTGCTATTCAA 676 431 333 Antisense
TATTCTACGATGGACCATTCCCCTG 612 661 383 Antisense
CCCTGGTCACTGATACTCTGTATTC 211 261 403 Antisense
GAGGTTGGCTGATATCCAGTCTGAT 339 403 482 Antisense
GTCTGATAGCTGCTGCATTGTCATT 408 471 500 Antisense
AATGTACGGCGCTATTTTTCTGGAC 683 163 530 Antisense
ATAGCATGTGGCCTTATTATTTGGA 329 23 679 Antisense
GCTACGAACACAGCTATCACCATCT 477 303 705 Antisense
GAGTGAACCAGAACTTGGTCACCAG 332 401 756 Antisense
Sequence Source Affymetrix Proprietary Database