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[182300_s_at]

Description F08E10.1 /REP_DB=WormBase Gene ID /WP=CE18571 /TR=Q9XXP3 /GB=CAA16501.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 79      4grp_total > 03      6grp_cgc > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 1.6
(-3)
A 		2.9
(-2.4)
A 		3.6
(-2.1)
A 		14.8
(3)
A 		13
B 4.3
(-2.6)
A 		8.1
(-1.5)
A 		3.1
(-3)
A 		23.2
(3)
A 		20
C 17.7
(-1.9)
A 		3.6
(-2.9)
A 		1.9
(-3)
A 		87
(3)
A 		85
avr 7.9
(-2.2) 		4.9
(-2.7) 		2.9
(-3) 		41.7
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 2.4
(-3)
A 		6
(-2.1)
A 		4.1
(-2.6)
A 		6.2
(-2.1)
A 		4.6
(-2.5)
A 		27.3
(3)
A 		25
B 2.7
(-2.4)
A 		2.7
(-2.4)
A 		1.6
(-3)
A 		1.9
(-2.8)
A 		13.4
(3)
A 		1.8
(-2.9)
A 		12
C 4.9
(-2.3)
A 		21.1
(3)
A 		2.8
(-3)
A 		4.7
(-2.4)
A 		3.9
(-2.6)
A 		4.5
(-2.4)
A 		18
avr 3.3
(-2.6) 		9.9
(2.1) 		2.8
(-3) 		4.3
(-1.9) 		7.3
(0.2) 		11.2
(3) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 0.6
(-3)
A 		0.6
(-3)
A 		1
(-1.5)
A 		2.2
(3)
A 		2
B 1.4
(-2)
A 		2
(-1.1)
A 		0.7
(-3)
A 		4.9
(3)
A 		4
C 5.9
(-0.7)
A 		1
(-2.8)
A 		0.6
(-3)
A 		14.7
(3)
A 		14
avr 2.6
(-1.3) 		1.2
(-2.6) 		0.8
(-3) 		7.3
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 0.4
(-3)
A 		2.2
(-1.3)
A 		0.9
(-2.5)
A 		1.6
(-1.9)
A 		1.4
(-2)
A 		6.7
(3)
A 		6
B 0.9
(-2.4)
A 		0.9
(-2.4)
A 		0.4
(-3)
A 		0.5
(-2.9)
A 		5.4
(3)
A 		0.7
(-2.6)
A 		5
C 1.8
(-1.9)
A 		6.5
(3)
A 		0.7
(-3)
A 		0.9
(-2.8)
A 		1.3
(-2.4)
A 		1.5
(-2.2)
A 		6
avr 1
(-2.3) 		3.2
(3) 		0.7
(-3) 		1
(-2.3) 		2.7
(1.8) 		3
(2.5) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182300_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14284
Sequence Type Exemplar sequence
Representative Public ID F08E10.1 NCBI
Target Description F08E10.1 /REP_DB=WormBase Gene ID /WP=CE18571 /TR=Q9XXP3 /GB=CAA16501.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074957(11), NM_075022(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074957 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-236) (srh-236) mRNA, complete cds. 11/11 A 
NM_075022 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-235) (srh-235) mRNA, complete cds. 11/11 A 
SNAP00000011466 ENSEMBL cdna:SNAP chromosome:CEL140:V:17213293:17214616:-1 11/11 None
GENEFINDER00000011467 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17212970:17214408:-1 11/11 None
SNAP00000039050 ENSEMBL cdna:SNAP chromosome:CEL140:V:17472799:17474108:-1 11/11 None
Y68A4A.9 ENSEMBL cdna:known chromosome:CEL140:V:17213293:17214616:-1 gene:Y68A4A.9 11/11 None
F08E10.1 ENSEMBL cdna:known chromosome:CEL140:V:17472799:17474240:-1 gene:F08E10.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17472798-17474240(-) 100.0 100.0
chrV:17213292-17214616(-) 92.91 92.91
Public Domain and Genome References
Gene Title

Gene Symbol srh-235
srh-236
Ensembl Y68A4A.9
F08E10.1
Entrez Gene 184182 Entrez gene
190528 Entrez gene
SwissProt Q9XXP3 EMBL-EBI
Q9XXQ3 EMBL-EBI
WormBase CE18571 Wormbase
CE36049 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA16416 Hypothetical protein Y68A4A.9 [Caenorhabditis elegans] ref|NP_507358.2| Serpentine Receptor, class H family member (srh-236) [Caenorhabditis elegans] 0.0
blast CAA16501 Hypothetical protein F08E10.1 [Caenorhabditis elegans] ref|NP_507423.1| Serpentine Receptor, class H family member (srh-235) [Caenorhabditis elegans] 0.0
blast CAA16501 Hypothetical protein F08E10.1 [Caenorhabditis elegans] ref|NP_507423.1| Serpentine Receptor, class H family member (srh-235) [Caenorhabditis elegans] 1.0E-177
blast CAA16416 Hypothetical protein Y68A4A.9 [Caenorhabditis elegans] ref|NP_507358.2| Serpentine Receptor, class H family member (srh-236) [Caenorhabditis elegans] 1.0E-177
blast CAA16416 Hypothetical protein Y68A4A.9 [Caenorhabditis elegans] ref|NP_507358.2| Serpentine Receptor, class H family member (srh-236) [Caenorhabditis elegans] 1.0E-173
blast CAA16501 Hypothetical protein F08E10.1 [Caenorhabditis elegans] ref|NP_507423.1| Serpentine Receptor, class H family member (srh-235) [Caenorhabditis elegans] 1.0E-162
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.0E-32
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 2.9E-92
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 5.8E-89
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.2E-122
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507358.2 7 15-37,58-80,90-112,133-152,191-213,234-256,269-288
NP_507423.1 7 15-37,58-80,90-112,133-152,193-215,235-257,272-291
Y68A4A.9 7 15-37,58-80,90-112,133-152,191-213,234-256,269-288
F08E10.1 7 15-37,58-80,90-112,133-152,193-215,235-257,272-291
SNAP00000011466 7 20-39,51-68,73-95,116-135,174-196,217-239,252-271
GENEFINDER00000011467 6 20-42,63-82,122-144,165-187,200-219,240-262
SNAP00000039050 6 9-31,46-68,89-108,149-171,191-213,228-247
Sequence
Target Sequence 
>C. ELEGANS:182300_S_AT
ttcagttatattattgtgccactctacgatttgccaattcaatttttgataccagaacag
agcaaaggaagggaactctcgtggaggaagctccaatgtattcctgaacttccaaatgac
ggccgtgagttgttcgttttcgccactgaacttcttggtccagcgatcacaattattctg
gcagagtctgtcccaactattcagtgtggaacatttctagcactcaacatttacaacttg
atattcgcaaggcgaagtgggatttccaagaagacagttcaaatgcaacacaggctcgtg
gtcgcttttatcatacagacatcagtgaccctaattctttttgtggtgcctgtcaatgca
ttcatttcatttatatactttaactatcaaaatcaatttcataacaatttgattgtcttt
gcatttgcggttcacggaattgcatccacacttatcatggtgtttgttcataggccatat
cgagactttgtctattcgccgtttcggcggctttttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCAGTTATATTATTGTGCCACTCT 140 689 418 Antisense
TATTGTGCCACTCTACGATTTGCCA 477 665 429 Antisense
GGAAGGGAACTCTCGTGGAGGAAGC 432 513 484 Antisense
GTGGAGGAAGCTCCAATGTATTCCT 668 489 498 Antisense
AGTGGGATTTCCAAGAAGACAGTTC 476 57 673 Antisense
GTCTTTGCATTTGCGGTTCACGGAA 459 469 832 Antisense
TTGCATTTGCGGTTCACGGAATTGC 28 699 836 Antisense
TTGTTCATAGGCCATATCGAGACTT 453 705 881 Antisense
ATCGAGACTTTGTCTATTCGCCGTT 127 35 896 Antisense
CTTTGTCTATTCGCCGTTTCGGCGG 449 219 903 Antisense
TATTCGCCGTTTCGGCGGCTTTTTG 158 653 910 Antisense
Sequence Source Affymetrix Proprietary Database