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[182299_at]

Description F08E10.1 /REP_DB=WormBase Gene ID /WP=CE18571 /TR=Q9XXP3 /GB=CAA16501.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 92      4grp_total > 07      6grp_cgc > 98      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 22.5
(3)
A 		1.7
(-3)
A 		1.7
(-3)
A 		3.6
(-2.5)
A 		21
B 4.1
(-2.7)
A 		1.9
(-3)
A 		2.1
(-3)
A 		46.2
(3)
A 		44
C 26
(3)
A 		6.8
(-3)
A 		25.1
(2.7)
A 		14.1
(-0.7)
A 		19
avr 17.5
(1.7) 		3.5
(-3) 		9.6
(-0.9) 		21.3
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 6.1
(-1.9)
A 		2.1
(-3)
A 		7.3
(-1.6)
A 		8.2
(-1.3)
A 		24.2
(3)
A 		7.5
(-1.5)
A 		22
B 8.3
(3)
A 		5.1
(-0.1)
A 		3.6
(-1.6)
A 		2.2
(-3)
A 		6.9
(1.6)
A 		3.2
(-2)
A 		6
C 2.4
(-3)
A 		19.7
(2.3)
A 		7.3
(-1.5)
A 		10.1
(-0.7)
A 		22.1
(3)
A 		2.7
(-2.9)
A 		20
avr 5.6
(-2.5) 		9
(-1) 		6.1
(-2.3) 		6.8
(-2) 		17.7
(3) 		4.5
(-3) 		13
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 8.2
(3)
A 		0.4
(-3)
A 		0.5
(-2.9)
A 		0.5
(-2.9)
A 		8
B 1.3
(-2.5)
A 		0.5
(-3)
A 		0.5
(-3)
A 		9.9
(3)
A 		9
C 8.6
(2.9)
A 		2
(-3)
A 		8.7
(3)
A 		2.4
(-2.6)
A 		7
avr 6
(3) 		1
(-3) 		3.2
(-0.4) 		4.3
(1) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 1.1
(-2.7)
A 		0.8
(-3)
A 		1.6
(-2.3)
A 		2.1
(-1.8)
A 		7.3
(3)
A 		1.8
(-2.1)
A 		7
B 2.9
(3)
A 		1.7
(-0.1)
A 		0.9
(-2.2)
A 		0.6
(-3)
A 		2.8
(2.7)
A 		1.2
(-1.4)
A 		2
C 0.9
(-3)
A 		6
(1.9)
A 		1.9
(-2)
A 		2
(-2)
A 		7.2
(3)
A 		0.9
(-3)
A 		6
avr 1.6
(-2.6) 		2.8
(-1) 		1.5
(-2.7) 		1.6
(-2.6) 		5.8
(3) 		1.3
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182299_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14284
Sequence Type Exemplar sequence
Representative Public ID F08E10.1 NCBI
Target Description F08E10.1 /REP_DB=WormBase Gene ID /WP=CE18571 /TR=Q9XXP3 /GB=CAA16501.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075022(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075022 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-235) (srh-235) mRNA, complete cds. 11/11 A 
SNAP00000039050 ENSEMBL cdna:SNAP chromosome:CEL140:V:17472799:17474108:-1 11/11 None
F08E10.1 ENSEMBL cdna:known chromosome:CEL140:V:17472799:17474240:-1 gene:F08E10.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17472798-17474240(-) 100.0 100.0
chrV:17213292-17214616(-) 92.91 92.91
Public Domain and Genome References
Gene Title
Gene Symbol srh-235
Ensembl F08E10.1
Entrez Gene 184182 Entrez gene
SwissProt Q9XXP3 EMBL-EBI
WormBase CE18571 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA16501 Hypothetical protein F08E10.1 [Caenorhabditis elegans] ref|NP_507423.1| Serpentine Receptor, class H family member (srh-235) [Caenorhabditis elegans] 0.0
blast CAA16416 Hypothetical protein Y68A4A.9 [Caenorhabditis elegans] ref|NP_507358.2| Serpentine Receptor, class H family member (srh-236) [Caenorhabditis elegans] 1.0E-177
blast CAA16501 Hypothetical protein F08E10.1 [Caenorhabditis elegans] ref|NP_507423.1| Serpentine Receptor, class H family member (srh-235) [Caenorhabditis elegans] 1.0E-162
blast CAB04854 Hypothetical protein T26H5.3 [Caenorhabditis elegans] ref|NP_506842.1| Serpentine Receptor, class H family member (srh-239) [Caenorhabditis elegans] 1.0E-150
blast CAA16416 Hypothetical protein Y68A4A.9 [Caenorhabditis elegans] ref|NP_507358.2| Serpentine Receptor, class H family member (srh-236) [Caenorhabditis elegans] 1.0E-149
blast CAB04854 Hypothetical protein T26H5.3 [Caenorhabditis elegans] ref|NP_506842.1| Serpentine Receptor, class H family member (srh-239) [Caenorhabditis elegans] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.2E-122
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507423.1 7 15-37,58-80,90-112,133-152,193-215,235-257,272-291
F08E10.1 7 15-37,58-80,90-112,133-152,193-215,235-257,272-291
SNAP00000039050 6 9-31,46-68,89-108,149-171,191-213,228-247
Sequence
Target Sequence 
>C. ELEGANS:182299_AT
tccaatgtattcctgaacttccaaatgacggccgtgagttgttcgttttcgccactgaac
ttcttggtccagcgatcacaattattctggcagagtctgtcccaactattcagtgtggaa
catttctagcactcaacatttacaacttgatattcgcaaggcgaagtgggatttccaaga
agacagttcaaatgcaacacaggctcgtggtcgcttttatcatacagacatcagtgaccc
taattctttttgtggtgcctgtcaatgcattcatttcatttatatactttaactatcaaa
atcaatttcataacaatttgattgtctttgcatttgcggttcacggaattgcatccacac
ttatcatggtgtttgttcataggccatatcgagactttgtctattcgccgtttcggcggc
tttttgaaagtgggcctactgtatacgtgatacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCAATGTATTCCTGAACTTCCAAA 511 585 509 Antisense
GAACTTCCAAATGACGGCCGTGAGT 84 349 523 Antisense
CTTCTTGGTCCAGCGATCACAATTA 461 221 568 Antisense
GAGTCTGTCCCAACTATTCAGTGTG 134 401 601 Antisense
TTCGCAAGGCGAAGTGGGATTTCCA 193 685 661 Antisense
CTCGTGGTCGCTTTTATCATACAGA 2 233 712 Antisense
ACATCAGTGACCCTAATTCTTTTTG 93 107 736 Antisense
GCCTGTCAATGCATTCATTTCATTT 447 283 765 Antisense
GGAATTGCATCCACACTTATCATGG 623 533 853 Antisense
TCGGCGGCTTTTTGAAAGTGGGCCT 168 605 921 Antisense
GTGGGCCTACTGTATACGTGATACC 499 479 938 Antisense
Sequence Source Affymetrix Proprietary Database