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[182294_at]

Description Y60A3A.8 /REP_DB=WormBase Gene ID /WP=CE24532 /TR=Q9U1Z5 /GB=CAB60396.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 49      4grp_total > 21      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 228
1 2 3 4 range
A 178.8
(3)
A 		89.4
(-0.2)
A 		13.4
(-3)
A 		150.2
(2)
A 		165
B 91.6
(0.3)
A 		86.2
(-0.5)
A 		67.6
(-3)
A 		111.8
(3)
A 		44
C 156.6
(-0.3)
A 		96.9
(-2.6)
A 		86.8
(-3)
A 		241.4
(3)
A 		155
avr 142.3
(1.6) 		90.8
(-1.1) 		55.9
(-3) 		167.8
(3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 164
1 2 3 4 5 6 range
A 241.5
(3)
M 		154.3
(-1.2)
A 		118
(-3)
P 		175.9
(-0.2)
A 		124.1
(-2.7)
A 		194.3
(0.7)
P 		124
B 125.5
(2.6)
A 		81.5
(-3)
A 		89.3
(-2)
A 		99.6
(-0.7)
A 		124.3
(2.4)
P 		128.8
(3)
P 		47
C 202.2
(3)
P 		77.7
(-3)
A 		140.9
(0)
A 		86.1
(-2.6)
A 		165.5
(1.2)
P 		201.5
(3)
P 		125
avr 189.7
(3) 		104.5
(-3) 		116.1
(-2.2) 		120.5
(-1.9) 		138
(-0.6) 		174.9
(2) 		85
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 65.1
(3)
A 		19.4
(-1.5)
A 		3.5
(-3)
A 		22
(-1.2)
A 		62
B 29.2
(3)
A 		21.3
(-0.5)
A 		15.7
(-3)
A 		23.8
(0.6)
A 		14
C 51.9
(3)
A 		27.9
(-3)
A 		30.2
(-2.4)
A 		40.7
(0.2)
A 		24
avr 48.7
(3) 		22.9
(-1.8) 		16.5
(-3) 		28.8
(-0.7) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 44.5
(0.9)
M 		54.9
(3)
A 		25.7
(-3)
P 		44.6
(0.9)
A 		37.5
(-0.6)
A 		47.3
(1.4)
P 		29
B 43.7
(1.6)
A 		27.1
(-1.9)
A 		21.7
(-3)
A 		26.4
(-2)
A 		50.5
(3)
P 		48
(2.5)
P 		29
C 74.9
(3)
P 		23.7
(-2.3)
A 		35.9
(-1.1)
A 		17.3
(-3)
A 		53.5
(0.8)
P 		67.5
(2.2)
P 		58
avr 54.4
(3) 		35.2
(-1.3) 		27.8
(-3) 		29.4
(-2.6) 		47.2
(1.4) 		54.3
(3) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182294_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14044
Sequence Type Exemplar sequence
Representative Public ID Y60A3A.8 NCBI
Target Description Y60A3A.8 /REP_DB=WormBase Gene ID /WP=CE24532 /TR=Q9U1Z5 /GB=CAB60396.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075461(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075461 NCBI Caenorhabditis elegans Y60A3A.8 (Y60A3A.8) mRNA, complete cds. 11/11 None
SNAP00000032803 ENSEMBL cdna:SNAP chromosome:CEL140:V:19950275:19955480:1 10/11 None
GENEFINDER00000032843 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19948009:19952989:1 11/11 None
Y60A3A.8 ENSEMBL cdna:known chromosome:CEL140:V:19947993:19952989:1 gene:Y60A3A.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032587 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19951628-19952989(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y60A3A.8
Entrez Gene 180308 Entrez gene
SwissProt Q9U1Z5 EMBL-EBI
WormBase CE32028 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60396 Hypothetical protein Y60A3A.8 [Caenorhabditis elegans] ref|NP_507862.2| Y60A3A.8 [Caenorhabditis elegans] 0.0
blast CAB60395 Hypothetical protein Y60A3A.7 [Caenorhabditis elegans] ref|NP_507863.1| Y60A3A.7 [Caenorhabditis elegans] 1.0E-129
blast AAP31440 Hypothetical protein C44C1.5b [Caenorhabditis elegans] ref|NP_001024459.1| C44C1.5b [Caenorhabditis elegans] 1.0E-104
blast CAE73131 Hypothetical protein CBG20515 [Caenorhabditis briggsae] 2.0E-70
blast CAB07417 Hypothetical protein T08D2.2 [Caenorhabditis elegans] ref|NP_508055.1| T08D2.2 [Caenorhabditis elegans] 4.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.3E-7
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 2.3E-5
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.3E-7
Sequence
Target Sequence 
>C. ELEGANS:182294_AT
caaagactcctagacctgatgacgtcatcattcccgaaaaaatcgataaaatccaagtca
ctagtcattcattggcatcaagaaacacaagaaatcgctgaaattctgcaagttttccac
acgaaaaccttgaaaaacttggaattccatgatagaaacgccacgtggcacatggcgccg
attatcggaacggagcagtggaagggagcacgatcggtgctcttggagcacctttcagat
gtgaaaattgaggaattgaaggattttaatgagattcaaatgcatgtgagggattcgggg
gagcaagagctttggacggtgattcagaatttcatccgaaaaaacctgcatggctcgtca
ttccatttcacatatcgtcaagctgagccgaatgtcgatcgaattcttgcaacatttaat
gttccgccaagagatgaaccaattactgccaggacacgattaagatccgtgctcggaagc
cgattcattcacactcaacgtttccagatgccatctactgatcttattctagtagtcatg
atttcagaaaaagaagtgatgggtacggtatgccacgtggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAGACTCCTAGACCTGATGACGT 232 193 70 Antisense
GTCACTAGTCATTCATTGGCATCAA 597 465 126 Antisense
GAAACGCCACGTGGCACATGGCGCC 204 359 224 Antisense
GCTCTTGGAGCACCTTTCAGATGTG 402 301 288 Antisense
AAAAAACCTGCATGGCTCGTCATTC 45 131 408 Antisense
TCGTCATTCCATTTCACATATCGTC 326 605 424 Antisense
GATGAACCAATTACTGCCAGGACAC 634 411 502 Antisense
GATCCGTGCTCGGAAGCCGATTCAT 508 415 533 Antisense
CGATTCATTCACACTCAACGTTTCC 337 247 550 Antisense
GATGCCATCTACTGATCTTATTCTA 256 415 576 Antisense
GATGGGTACGGTATGCCACGTGGAT 611 405 627 Antisense
Sequence Source Affymetrix Proprietary Database