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[182292_at]

Description Y87G2A.4 /REP_DB=WormBase Gene ID /WP=CE23130 /TR=Q9NA29 /GB=CAB54484.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 05      4grp_highest_8_8 > 53      4grp_high_8_8 > 18      6grp_highest_nonorm > 50      6grp_total > 06      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 12      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 355
1 2 3 4 range
A 732.5
(3)
P 		523
(-1.8)
P 		471.9
(-3)
P 		643.2
(0.9)
P 		261
B 738.9
(3)
P 		462
(-2.7)
P 		447.7
(-3)
P 		638.5
(0.9)
P 		291
C 688.2
(3)
P 		526.2
(-0.2)
P 		383.9
(-3)
P 		682.2
(2.9)
P 		304
avr 719.9
(3) 		503.7
(-1.5) 		434.5
(-3) 		654.6
(1.6) 		285

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 431
1 2 3 4 5 6 range
A 652
(3)
P 		532.4
(0.9)
P 		458.3
(-0.5)
P 		404.4
(-1.4)
P 		431.8
(-0.9)
P 		315.9
(-3)
P 		336
B 607.4
(3)
P 		495.3
(0.7)
P 		396.2
(-1.4)
P 		377.3
(-1.8)
P 		450.5
(-0.3)
P 		320.8
(-3)
P 		287
C 747
(3)
P 		446.7
(-1.3)
P 		455.7
(-1.1)
P 		323.5
(-3)
P 		409.6
(-1.8)
P 		334
(-2.9)
P 		424
avr 668.8
(3) 		491.5
(-0.1) 		436.7
(-1) 		368.4
(-2.2) 		430.6
(-1.1) 		323.6
(-3) 		345
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 173
1 2 3 4 range
A 266.7
(3)
P 		113.5
(-2.3)
P 		123.4
(-2)
P 		94.2
(-3)
P 		173
B 235.6
(3)
P 		114.1
(-2.5)
P 		103.7
(-3)
P 		136.1
(-1.5)
P 		132
C 227.8
(3)
P 		151.3
(-1.1)
P 		133.8
(-2)
P 		115
(-3)
P 		113
avr 243.4
(3) 		126.3
(-2.5) 		120.3
(-2.8) 		115.1
(-3) 		128

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 212
1 2 3 4 5 6 range
A 120.2
(-0.7)
P 		189.3
(3)
P 		99.8
(-1.8)
P 		102.6
(-1.6)
P 		130.3
(-0.1)
P 		76.9
(-3)
P 		112
B 211.4
(3)
P 		164.9
(0.6)
P 		96.2
(-3)
P 		99.9
(-2.8)
P 		183
(1.5)
P 		119.6
(-1.8)
P 		115
C 276.9
(3)
P 		136.3
(-1)
P 		116
(-1.6)
P 		65
(-3)
P 		132.5
(-1.1)
P 		111.8
(-1.7)
P 		212
avr 202.8
(3) 		163.5
(0.9) 		104
(-2.2) 		89.2
(-3) 		148.6
(0.1) 		102.8
(-2.3) 		114
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182292_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2076
Sequence Type Exemplar sequence
Representative Public ID Y87G2A.4 NCBI
Target Description Y87G2A.4 /REP_DB=WormBase Gene ID /WP=CE23130 /TR=Q9NA29 /GB=CAB54484.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060975(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060975 NCBI Caenorhabditis elegans RAB family member (rab-27) (rab-27) mRNA, complete cds. 11/11 A 
SNAP00000019586 ENSEMBL cdna:SNAP chromosome:CEL140:I:13538297:13555218:-1 9/11 None
GENEFINDER00000019615 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13551507:13553303:-1 11/11 None
Y87G2A.4 ENSEMBL cdna:known chromosome:CEL140:I:13551213:13553307:-1 gene:Y87G2A.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13551437-13553234(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rab-27
Ensembl Y87G2A.4
Entrez Gene 173223 Entrez gene
SwissProt Q9NA29 EMBL-EBI
WormBase CE23130 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.24390.1.S1_AT similar to Ras-related protein Rab-27A (Rab-27) (GTP-binding protein Ram) (Ram p25) cfa
DROSOPHILA_2:1629621_A_AT dm
DROSGENOME1:153277_AT dm
CHICKEN:GGAAFFX.2750.1.S1_S_AT similar to Ras-related protein Rab-27A (Rab-27) (GTP-binding protein Ram) (Ram p25) gga
HG-U133_PLUS_2:210951_X_AT RAB27A, member RAS oncogene family hs
HG-U133A:210951_X_AT RAB27A, member RAS oncogene family hs
HG-U133A_2:210951_X_AT RAB27A, member RAS oncogene family hs
HG-U95AV2:809_AT RAB27A, member RAS oncogene family hs
HC-G110:809_AT RAB27A, member RAS oncogene family hs
HG-U133A:209515_S_AT RAB27A, member RAS oncogene family hs
HG-U133_PLUS_2:209515_S_AT RAB27A, member RAS oncogene family hs
HG-U133A_2:209515_S_AT RAB27A, member RAS oncogene family hs
HU35KSUBC:RC_AA278355_AT RAB27A, member RAS oncogene family hs
HU35KSUBD:RC_N73083_AT RAB27A, member RAS oncogene family hs
HUGENEFL:U57094_AT RAB27A, member RAS oncogene family hs
U133_X3P:G4887230_3P_A_AT RAB27A, member RAS oncogene family hs
U133_X3P:HS.50477.1.S1_3P_A_AT RAB27A, member RAS oncogene family hs
HG-U133A_2:209514_S_AT RAB27A, member RAS oncogene family hs
HG-FOCUS:209514_S_AT RAB27A, member RAS oncogene family hs
HG-U133_PLUS_2:209514_S_AT RAB27A, member RAS oncogene family hs
HG-U133A:209514_S_AT RAB27A, member RAS oncogene family hs
HG-U95AV2:40669_AT RAB27A, member RAS oncogene family hs
HG-U95C:65080_R_AT RAB27A, member RAS oncogene family hs
HG-U95E:68324_AT RAB27A, member RAS oncogene family hs
HG-U95E:86890_AT RAB27A, member RAS oncogene family hs
HG-U95E:89586_S_AT RAB27A, member RAS oncogene family hs
MOUSE430_2:1425284_A_AT RAB27A, member RAS oncogene family mm
MOUSE430A_2:1425284_A_AT RAB27A, member RAS oncogene family mm
MOE430A:1425284_A_AT RAB27A, member RAS oncogene family mm
MOUSE430A_2:1425285_A_AT RAB27A, member RAS oncogene family mm
MOUSE430_2:1425285_A_AT RAB27A, member RAS oncogene family mm
MOE430A:1425285_A_AT RAB27A, member RAS oncogene family mm
MG-U74BV2:163478_AT RAB27A, member RAS oncogene family mm
MOE430B:1429123_AT RAB27A, member RAS oncogene family mm
MOUSE430_2:1429123_AT RAB27A, member RAS oncogene family mm
MOE430A:1454438_AT RAB27A, member RAS oncogene family mm
MOUSE430A_2:1454438_AT RAB27A, member RAS oncogene family mm
MOUSE430_2:1454438_AT RAB27A, member RAS oncogene family mm
MU19KSUBC:TC41274_AT RAB27A, member RAS oncogene family, mRNA (cDNA clone MGC:6415 IMAGE:3590089) mm
RAE230A:1387763_AT low Mr GTP-binding protein rn
RAT230_2:1387763_AT low Mr GTP-binding protein rn
RG-U34A:D17352_AT low Mr GTP-binding protein rn
RG-U34B:RC_AI043615_AT low Mr GTP-binding protein rn
RG-U34C:RC_AI237416_AT low Mr GTP-binding protein rn
RAE230B:1377669_AT low Mr GTP-binding protein rn
RAT230_2:1377669_AT low Mr GTP-binding protein rn
RAE230B:1384852_AT low Mr GTP-binding protein rn
RAT230_2:1384852_AT low Mr GTP-binding protein rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB54483 Hypothetical protein Y87G2A.3 [Caenorhabditis elegans] ref|NP_493375.1| Y87G2A.3 [Caenorhabditis elegans] 0.0
blast CAE72613 Hypothetical protein CBG19805 [Caenorhabditis briggsae] 1.0E-156
blast CAB54484 Hypothetical protein Y87G2A.4 [Caenorhabditis elegans] ref|NP_493376.1| RAB family member (rab-27) [Caenorhabditis elegans] dbj|BAD07035.1| Rab27 [Caenorhabditis elegans] 1.0E-113
blast CAE63548 Hypothetical protein CBG08034 [Caenorhabditis briggsae] 1.0E-109
blast CAB54484 Hypothetical protein Y87G2A.4 [Caenorhabditis elegans] ref|NP_493376.1| RAB family member (rab-27) [Caenorhabditis elegans] dbj|BAD07035.1| Rab27 [Caenorhabditis elegans] 1.0E-81
blast AAH82420 LOC494641 protein [Xenopus laevis] 2.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 5.7E-73
Pfam IPR005078 EMBL-EBI Peptidase C54 6.7E-43
Pfam IPR005078 EMBL-EBI Peptidase C54 9.0E-30
Pfam IPR005078 EMBL-EBI Peptidase C54 3.0E-8
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 2.7E-85
Sequence
Target Sequence 
>C. ELEGANS:182292_AT
gttatgggacactgccggacaagagagattccgttctctaacaacagcgttcttccgaga
tgcaatgggatttatattgatcttcgatattacaaatgagcaatcatttctcaatattcg
agactggctatcacaattgaaggtacatgcctattgcgagcaacctgacattatcatatg
cggtaacaaagcagacctcgaaaatcggcgtcaagtcagtaccgcccgtgcaaagcagct
ggctgatcaattggggctgccgtacttcgagacgtccgcctgcacatctaccaatgtgga
gaaatctgtggattgtctactcgatttggttatgcaaagaattcaacaatctgttgagac
gtcgtctcttccgttgtcagaatgccgcggagtcagcttggacggagaccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTATGGGACACTGCCGGACAAGAG 100 445 216 Antisense
GATTCCGTTCTCTAACAACAGCGTT 507 433 242 Antisense
AACAGCGTTCTTCCGAGATGCAATG 528 135 258 Antisense
GGTACATGCCTATTGCGAGCAACCT 220 503 357 Antisense
AATCGGCGTCAAGTCAGTACCGCCC 176 167 418 Antisense
GCCCGTGCAAAGCAGCTGGCTGATC 346 287 439 Antisense
CAATTGGGGCTGCCGTACTTCGAGA 321 179 463 Antisense
GTGGATTGTCTACTCGATTTGGTTA 80 491 523 Antisense
GTTGAGACGTCGTCTCTTCCGTTGT 57 439 568 Antisense
GTTGTCAGAATGCCGCGGAGTCAGC 710 431 588 Antisense
CGGAGTCAGCTTGGACGGAGACCCA 153 243 603 Antisense
Sequence Source Affymetrix Proprietary Database