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[182286_at]

Description F45E1.4 /REP_DB=WormBase Gene ID /WP=CE01941 /TR=Q09987 /GB=AAB04904.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 82      4grp_high_8_8 > 23      6grp_highest_nonorm > 72      6grp_total > 73      4grp_total > 24      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 809
1 2 3 4 range
A 40.6
(-3)
A 		251
(-1.1)
P 		414.4
(0.4)
P 		703.6
(3)
P 		663
B 68.9
(-3)
A 		326.2
(-1)
P 		505.4
(0.4)
P 		849.9
(3)
P 		781
C 102.4
(-3)
P 		279.2
(-1.2)
P 		560.7
(1.7)
P 		688.9
(3)
P 		587
avr 70.6
(-3) 		285.5
(-1.1) 		493.5
(0.7) 		747.5
(3) 		677

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 563
1 2 3 4 5 6 range
A 107.7
(-3)
P 		348.9
(-0.3)
P 		486.9
(1.3)
P 		634.8
(3)
P 		446.2
(0.9)
P 		257.7
(-1.3)
P 		527
B 92.7
(-3)
P 		271.8
(-1.1)
P 		460.4
(0.9)
P 		472.6
(1)
P 		655.9
(3)
P 		283.6
(-1)
P 		563
C 125.1
(-3)
P 		234.6
(-1.4)
P 		484
(2.1)
P 		447.4
(1.6)
P 		544.3
(3)
P 		333.7
(-0)
P 		419
avr 108.5
(-3) 		285.1
(-0.6) 		477.1
(2) 		518.3
(2.6) 		548.8
(3) 		291.7
(-0.5) 		440
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 14.8
(-3)
A 		54.5
(-0.5)
P 		108.3
(3)
P 		103
(2.7)
P 		94
B 22
(-3)
A 		80.6
(-0.8)
P 		117.1
(0.6)
P 		181.1
(3)
P 		159
C 33.9
(-3)
P 		80.3
(-1.3)
P 		195.4
(3)
P 		116.2
(0.1)
P 		162
avr 23.6
(-3) 		71.8
(-0.5) 		140.3
(3) 		133.4
(2.6) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 247
1 2 3 4 5 6 range
A 19.8
(-3)
P 		124.1
(1.4)
P 		106
(0.7)
P 		161
(3)
P 		134.7
(1.9)
P 		62.7
(-1.2)
P 		141
B 32.3
(-3)
P 		90.5
(-1.5)
P 		111.8
(-1)
P 		125.2
(-0.6)
P 		266.5
(3)
P 		105.8
(-1.1)
P 		234
C 46.4
(-3)
P 		71.6
(-1.8)
P 		123.2
(0.6)
P 		90
(-1)
P 		176.1
(3)
P 		111.7
(0)
P 		130
avr 32.8
(-3) 		95.4
(-0.6) 		113.7
(0) 		125.4
(0.5) 		192.4
(3) 		93.4
(-0.7) 		160
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182286_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16528
Sequence Type Exemplar sequence
Representative Public ID F45E1.4 NCBI
Target Description F45E1.4 /REP_DB=WormBase Gene ID /WP=CE01941 /TR=Q09987 /GB=AAB04904.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076944(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076944 NCBI Caenorhabditis elegans F45E1.4 (F45E1.4) mRNA, complete cds. 11/11 None
GENEFINDER00000004158 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7982559:7988750:1 11/11 None
SNAP00000004148 ENSEMBL cdna:SNAP chromosome:CEL140:X:7985924:7986169:1 11/11 None
F45E1.4 ENSEMBL cdna:known chromosome:CEL140:X:7985924:7986169:1 gene:F45E1.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7985922-7986168(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45E1.4
Entrez Gene 185793 Entrez gene
SwissProt Q09987 EMBL-EBI
WormBase CE01941 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB04900 Hypothetical protein F45E1.3 [Caenorhabditis elegans] ref|NP_509346.1| F45E1.3 [Caenorhabditis elegans] 1.0E-144
blast CAE70331 Hypothetical protein CBG16864 [Caenorhabditis briggsae] 6.0E-98
blast CAB07180 Hypothetical protein F09C3.2 [Caenorhabditis elegans] ref|NP_493493.2| F09C3.2 [Caenorhabditis elegans] 6.0E-31
blast AAB04904 Hypothetical protein F45E1.4 [Caenorhabditis elegans] ref|NP_509345.1| F45E1.4 [Caenorhabditis elegans] 3.0E-30
blast CAE70331 Hypothetical protein CBG16864 [Caenorhabditis briggsae] 2.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 2.3E-20
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000004158 2 119-137,147-166
Sequence
Target Sequence 
>C. ELEGANS:182286_AT
gtttgccactaaaactggagctcatcttgcgtcagctattaccaagagcgcgttccaaca
aagatggtccagcgcagtttccacaagctaccgcaagactcgggaaatggcctcactcgg
tggagcggagcacgaatctccgtctcttgtcatttttgaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTGCCACTAAAACTGGAGCTCAT 272 451 15 Antisense
GAGCTCATCTTGCGTCAGCTATTAC 33 387 32 Antisense
GCGTCAGCTATTACCAAGAGCGCGT 9 291 43 Antisense
CTATTACCAAGAGCGCGTTCCAACA 701 221 50 Antisense
GAGCGCGTTCCAACAAAGATGGTCC 22 387 60 Antisense
CAAAGATGGTCCAGCGCAGTTTCCA 25 193 73 Antisense
TTCCACAAGCTACCGCAAGACTCGG 208 701 93 Antisense
CAAGACTCGGGAAATGGCCTCACTC 231 185 108 Antisense
CTCACTCGGTGGAGCGGAGCACGAA 272 229 126 Antisense
GAGCGGAGCACGAATCTCCGTCTCT 139 387 137 Antisense
TCTCCGTCTCTTGTCATTTTTGACA 338 611 151 Antisense
Sequence Source Affymetrix Proprietary Database