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[182273_at]

Description R12B2.2 /REP_DB=WormBase Gene ID /WP=CE01365 /TR=Q21954 /GB=AAA50740.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 23      4grp_highest_8_8 > 31      4grp_high_8_8 > 20      6grp_highest_nonorm > 90      6grp_total > 03      4grp_total > 19      6grp_highest_sub > 04      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 208
1 2 3 4 range
A 169.9
(-1.5)
P 		124.9
(-2.8)
P 		119.3
(-3)
P 		327.7
(3)
P 		208
B 204.3
(0.2)
P 		238.8
(3)
P 		165.4
(-3)
P 		234.5
(2.6)
P 		73
C 203
(-0.8)
P 		270.7
(2.1)
P 		150.6
(-3)
P 		290.9
(3)
P 		140
avr 192.4
(-1) 		211.5
(-0.1) 		145.1
(-3) 		284.4
(3) 		139

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 183.8
(0.4)
P 		196.9
(1)
P 		241.9
(3)
P 		185.2
(0.4)
P 		188.2
(0.6)
P 		108.9
(-3)
P 		133
B 199.2
(0.5)
P 		220.3
(1.8)
P 		138.7
(-3)
P 		241.3
(3)
P 		197.7
(0.5)
P 		162.1
(-1.6)
P 		103
C 188.5
(2.2)
P 		200.1
(3)
P 		183.1
(1.8)
P 		165.5
(0.5)
P 		198.4
(2.9)
P 		118.2
(-3)
P 		82
avr 190.5
(1.8) 		205.8
(3) 		187.9
(1.6) 		197.3
(2.3) 		194.8
(2.1) 		129.7
(-3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 61.9
(3)
P 		27.1
(-3)
P 		31.2
(-2.3)
P 		48
(0.6)
P 		35
B 65.1
(3)
P 		59
(1.6)
P 		38.3
(-3)
P 		50
(-0.4)
P 		27
C 67.2
(0.8)
P 		77.8
(3)
P 		52.5
(-2.3)
P 		49
(-3)
P 		29
avr 64.7
(3) 		54.6
(0.5) 		40.7
(-3) 		49
(-0.9) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 33.9
(-2)
P 		70
(3)
P 		52.7
(0.6)
P 		47
(-0.2)
P 		56.8
(1.2)
P 		26.5
(-3)
P 		44
B 69.3
(1.6)
P 		73.3
(2.1)
P 		33.7
(-3)
P 		63.9
(0.9)
P 		80.3
(3)
P 		60.5
(0.5)
P 		47
C 69.9
(3)
P 		61
(1.5)
P 		46.6
(-0.8)
P 		33.3
(-3)
P 		64.2
(2.1)
P 		39.6
(-2)
P 		37
avr 57.7
(0.6) 		68.1
(3) 		44.3
(-2.5) 		48.1
(-1.6) 		67.1
(2.8) 		42.2
(-3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182273_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6674
Sequence Type Exemplar sequence
Representative Public ID R12B2.2 NCBI
Target Description R12B2.2 /REP_DB=WormBase Gene ID /WP=CE01365 /TR=Q21954 /GB=AAA50740.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065854(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065854 NCBI Caenorhabditis elegans R12B2.2 (R12B2.2) mRNA, complete cds. 11/11 None
SNAP00000015242 ENSEMBL cdna:SNAP chromosome:CEL140:III:5805559:5809040:1 11/11 None
GENEFINDER00000015251 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5805648:5809040:1 11/11 None
R12B2.2 ENSEMBL cdna:known chromosome:CEL140:III:5807768:5809076:1 gene:R12B2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5807766-5809039(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R12B2.2
Entrez Gene 187835 Entrez gene
SwissProt Q21954 EMBL-EBI
WormBase CE01365 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA50741 Hypothetical protein R12B2.3 [Caenorhabditis elegans] 3.0E-99
blast AAA50740 Hypothetical protein R12B2.2 [Caenorhabditis elegans] ref|NP_498255.1| R12B2.2 [Caenorhabditis elegans] 9.0E-85
blast AAA50740 Hypothetical protein R12B2.2 [Caenorhabditis elegans] ref|NP_498255.1| R12B2.2 [Caenorhabditis elegans] 5.0E-84
blast AAA50741 Hypothetical protein R12B2.3 [Caenorhabditis elegans] 3.0E-80
blast CAE64398 Hypothetical protein CBG09088 [Caenorhabditis briggsae] 4.0E-78
blast CAE64398 Hypothetical protein CBG09088 [Caenorhabditis briggsae] 2.0E-77
Sequence
Target Sequence 
>C. ELEGANS:182273_AT
ccggtgacttcgtccgaacgagaagctgagttggaacgagaattggatcttctaagaaaa
gagttaaatattgaacaaaaaatgaattcggagcttaaaagattaatgattgccacgttg
agtgatgaacttcaggggcaggttgaagcattgacagaagataaagtacgattggctcat
cgagttgatgaatatatgggaaagctaatggttgaagatgaagaatcagatagactacga
attgatcgagatgtttggaaatgtaaatttttagctcaatcgatacgatgtgatgagcta
acttcaaagaatgagtatctattgaagacattggtaggcattcaacaagctgtaaaaact
aagaatgtaacttcggacgaaatcagtgattttacccaactcgaccttcaaccacttttt
caccgttctccttgtgaagaaagagttcgtccaattactgctaagtatacaaacctaaca
atcgcctgctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGGTGACTTCGTCCGAACGAGAAG 533 261 19 Antisense
GATTAATGATTGCCACGTTGAGTGA 49 431 119 Antisense
GAGTGATGAACTTCAGGGGCAGGTT 611 401 138 Antisense
GGGGCAGGTTGAAGCATTGACAGAA 94 499 153 Antisense
AATTTTTAGCTCAATCGATACGATG 83 175 284 Antisense
CGATACGATGTGATGAGCTAACTTC 77 249 299 Antisense
GACATTGGTAGGCATTCAACAAGCT 109 367 345 Antisense
TCAGTGATTTTACCCAACTCGACCT 46 607 401 Antisense
CTTTTTCACCGTTCTCCTTGTGAAG 335 219 433 Antisense
GAGTTCGTCCAATTACTGCTAAGTA 153 397 461 Antisense
ATACAAACCTAACAATCGCCTGCTG 428 23 485 Antisense
Sequence Source Affymetrix Proprietary Database