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[182259_at]

Description T12F5.2 /REP_DB=WormBase Gene ID /WP=CE13597 /TR=O44759 /GB=AAB96744.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 12      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 26.4
(-2.3)
A 		24.9
(-2.4)
A 		11.5
(-3)
A 		138.5
(3)
P 		127
B 56.7
(3)
A 		4
(-2.9)
A 		2.8
(-3)
A 		33.2
(0.4)
A 		54
C 36.1
(-3)
A 		36
(-3)
A 		40.1
(-2.3)
P 		71.4
(3)
P 		35
avr 39.7
(-0.9) 		21.6
(-2.7) 		18.1
(-3) 		81
(3) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 220
1 2 3 4 5 6 range
A 21.3
(-3)
A 		53.8
(-1.9)
A 		33
(-2.6)
A 		87.4
(-0.7)
P 		136
(1)
P 		191.3
(3)
P 		170
B 25.5
(-3)
A 		42.6
(-2.4)
A 		24.6
(-3)
A 		68.5
(-1.6)
P 		164.1
(1.4)
P 		212.8
(3)
P 		188
C 24
(-3)
A 		73.7
(-1.6)
P 		72.6
(-1.7)
P 		94.6
(-1.1)
P 		116.6
(-0.4)
P 		241.4
(3)
P 		217
avr 23.6
(-3) 		56.7
(-2) 		43.4
(-2.4) 		83.5
(-1.1) 		138.9
(0.6) 		215.2
(3) 		192
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 9.6
(-0.7)
A 		5.4
(-2.2)
A 		3
(-3)
A 		20.3
(3)
P 		17
B 18.1
(3)
A 		1
(-2.9)
A 		0.7
(-3)
A 		7.1
(-0.8)
A 		17
C 12
(-0.2)
A 		10.3
(-3)
A 		14
(3)
P 		12
(-0.2)
P 		4
avr 13.2
(3) 		5.6
(-3) 		5.9
(-2.8) 		13.1
(2.9) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 3.9
(-3)
A 		19.1
(-0.9)
A 		7.2
(-2.5)
A 		22.2
(-0.4)
P 		41
(2.2)
P 		46.6
(3)
P 		43
B 8.9
(-2.8)
A 		14.2
(-2.3)
A 		6
(-3)
A 		18.1
(-2)
P 		66.7
(2)
P 		79.4
(3)
P 		73
C 8.9
(-3)
A 		22.5
(-1.9)
P 		18.5
(-2.2)
P 		19
(-2.2)
P 		37.7
(-0.6)
P 		80.8
(3)
P 		72
avr 7.2
(-3) 		18.6
(-1.9) 		10.6
(-2.7) 		19.8
(-1.8) 		48.5
(1) 		68.9
(3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182259_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.253
Sequence Type Exemplar sequence
Representative Public ID T12F5.2 NCBI
Target Description T12F5.2 /REP_DB=WormBase Gene ID /WP=CE13597 /TR=O44759 /GB=AAB96744.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058807(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058807 NCBI Caenorhabditis elegans T12F5.2 (T12F5.2) mRNA, complete cds. 11/11 None
SNAP00000015202 ENSEMBL cdna:SNAP chromosome:CEL140:I:3731373:3732836:1 11/11 None
GENEFINDER00000015209 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3731792:3732836:1 11/11 None
T12F5.2 ENSEMBL cdna:known chromosome:CEL140:I:3731354:3732924:1 gene:T12F5.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075657 6/11 Cross Hyb Matching Probes None
SNAP00000032584 6/11 Cross Hyb Matching Probes None
GENEFINDER00000032589 7/11 Cross Hyb Matching Probes None
T08D2.5 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3731372-3732836(+) 100.0 100.0
chrX:178998-180888(-) 98.89 98.89
Public Domain and Genome References
Gene Title
Ensembl T12F5.2
Entrez Gene 188456 Entrez gene
SwissProt O44759 EMBL-EBI
WormBase CE13597 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB96744 Hypothetical protein T12F5.2 [Caenorhabditis elegans] ref|NP_491208.1| T12F5.2 [Caenorhabditis elegans] 0.0
blast AAB96744 Hypothetical protein T12F5.2 [Caenorhabditis elegans] ref|NP_491208.1| T12F5.2 [Caenorhabditis elegans] 1.0E-159
blast CAB07414 Hypothetical protein T08D2.5 [Caenorhabditis elegans] ref|NP_508058.1| T08D2.5 [Caenorhabditis elegans] 1.0E-140
blast CAE66655 Hypothetical protein CBG11992 [Caenorhabditis briggsae] 6.0E-43
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491208.1 2 266-288,309-328
T12F5.2 2 266-288,309-328
SNAP00000015202 2 266-288,309-328
GENEFINDER00000015209 2 216-238,259-278
Sequence
Target Sequence 
>C. ELEGANS:182259_AT
gtgcggatgtcctgaagactcgcgttcacaaggacttctccgacgcaatgaccattctgt
catcgttgccgattcttgtcaagaagaaggaatgcgaggttgccaagatagcggaattca
tgacaagagccaaaaatacggagagtctcactcaaattttcaagactttcgagagggaga
tgaagaatatcataaaaattctggaagctttgaaaagagatggtggaaagctgaaagata
ccgtggaacagactgtcaagcgacttgaagatgacaatgagaagatcgacatagcacact
gtcttcaagaatttgtcgatttgaaggagcatctcaagattccagaaatggttcagattc
cacaagacactgatcttccgattattgacgagacacttcaaaaggaggaatccaaaaaat
acaccattggaagtgtcatcgtctcgattttcagtgcaatcttcaattggctgtgcatgt
tcttcaaatttatttcgcaaatgattttcgggaccggaagcacttattataagaatgtct
ctactgaacacatcaaccttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCGGATGTCCTGAAGACTCGCGT 290 479 383 Antisense
GCGTTCACAAGGACTTCTCCGACGC 225 285 404 Antisense
TCTCCGACGCAATGACCATTCTGTC 79 611 419 Antisense
CTGTCATCGTTGCCGATTCTTGTCA 5 241 439 Antisense
GAGAGTCTCACTCAAATTTTCAAGA 620 393 523 Antisense
AGATCGACATAGCACACTGTCTTCA 22 47 665 Antisense
GACACTGATCTTCCGATTATTGACG 539 367 748 Antisense
CCATTGGAAGTGTCATCGTCTCGAT 192 271 806 Antisense
TTCAATTGGCTGTGCATGTTCTTCA 496 683 844 Antisense
GATTTTCGGGACCGGAAGCACTTAT 232 427 885 Antisense
GTCTCTACTGAACACATCAACCTTC 654 469 919 Antisense
Sequence Source Affymetrix Proprietary Database