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[182257_at] laminin B

Description C43H8.3 /REP_DB=WormBase Gene ID /WP=CE19379 /TR=Q9TZN3 /GB=AAC67398.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 75      6grp_total > 66      4grp_total > 40      6grp_cgc > 57      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 32.6
(-3)
A 		107
(0.4)
P 		162.4
(3)
P 		112.2
(0.7)
P 		130
B 56.4
(-3)
A 		137.8
(1.4)
P 		167.5
(3)
P 		92.7
(-1)
P 		111
C 68.6
(-3)
A 		186.2
(3)
P 		166.2
(2)
P 		91.7
(-1.8)
P 		118
avr 52.5
(-3) 		143.7
(1.8) 		165.4
(3) 		98.9
(-0.5) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 311
1 2 3 4 5 6 range
A 55.6
(-3)
A 		135.2
(-1.2)
P 		176.2
(-0.2)
P 		164.3
(-0.5)
P 		138.2
(-1.1)
P 		314
(3)
P 		258
B 115.9
(-3)
P 		150
(-2.2)
P 		197.8
(-1)
P 		115.8
(-3)
P 		133.1
(-2.6)
P 		366.1
(3)
P 		250
C 102.9
(-3)
P 		182.2
(-0.2)
P 		271.4
(3)
P 		160.4
(-1)
P 		131.6
(-2)
P 		260.1
(2.6)
P 		169
avr 91.5
(-3) 		155.8
(-1.3) 		215.1
(0.3) 		146.8
(-1.5) 		134.3
(-1.8) 		313.4
(3) 		222
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 11.9
(-3)
A 		23.2
(-0.8)
P 		42.4
(3)
P 		16.4
(-2.1)
P 		31
B 18
(-3)
A 		34
(1.6)
P 		38.8
(3)
P 		19.8
(-2.5)
P 		21
C 22.7
(-2)
A 		53.5
(2.4)
P 		57.9
(3)
P 		15.5
(-3)
P 		42
avr 17.5
(-2.9) 		36.9
(1) 		46.4
(3) 		17.2
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 10.2
(-3)
A 		48.1
(0.4)
P 		38.4
(-0.4)
P 		41.7
(-0.1)
P 		41.7
(-0.1)
P 		76.4
(3)
P 		66
B 40.3
(-2.5)
P 		49.9
(-1.9)
P 		48
(-2)
P 		30.7
(-3)
P 		54.1
(-1.7)
P 		136.5
(3)
P 		106
C 38.1
(-2.4)
P 		55.6
(-0.4)
P 		69.1
(1)
P 		32.3
(-3)
P 		42.6
(-1.9)
P 		87.1
(3)
P 		55
avr 29.5
(-3) 		51.2
(-1.2) 		51.8
(-1.1) 		34.9
(-2.5) 		46.1
(-1.6) 		100
(3) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182257_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2225
Sequence Type Exemplar sequence
Representative Public ID C43H8.3 NCBI
Target Description C43H8.3 /REP_DB=WormBase Gene ID /WP=CE19379 /TR=Q9TZN3 /GB=AAC67398.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060374(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060374 NCBI Caenorhabditis elegans LAMinin related. See also lmb- family member (lam-3) (lam-3) mRNA, complete cds. 11/11 A 
SNAP00000019931 ENSEMBL cdna:SNAP chromosome:CEL140:I:10627495:10629434:1 11/11 None
GENEFINDER00000019936 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10627495:10629434:1 11/11 None
T22A3.8 ENSEMBL cdna:known chromosome:CEL140:I:10616587:10629548:1 gene:T22A3.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10627425-10629365(+) 100.0 100.0
Public Domain and Genome References
Gene Title laminin B
Gene Symbol lam-3
Ensembl T22A3.8
Entrez Gene 172952 Entrez gene
SwissProt O45614 EMBL-EBI
WormBase CE31067 Wormbase
Functional Annotations
Ortholog
ID Title Organism
HG-U133_PLUS_2:205116_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133A:205116_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-FOCUS:205116_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133A_2:205116_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133A_2:213519_S_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133_PLUS_2:213519_S_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133A:213519_S_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HUGENEFL:Z26653_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
U133_X3P:G4557708_3P_S_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133A_2:216840_S_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133_PLUS_2:216840_S_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133A:216840_S_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U95AV2:36917_AT laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HU35KSUBC:RC_H88953_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
U133_X3P:HS2.378709.1.S1_3P_X_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
U133_X3P:HS2.378709.1.S1_3P_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
U133_X3P:HS2.330376.1.S1_3P_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
U133_X3P:HS.296739.0.S1_3P_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133_PLUS_2:1560135_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133_PLUS_2:1560997_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133_PLUS_2:1560998_X_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133A_2:216839_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133_PLUS_2:216839_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133A:216839_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133_PLUS_2:234099_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133B:234099_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U95B:47246_R_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133_PLUS_2:244339_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U133B:244339_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U95C:60954_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HG-U95D:68933_R_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
HU35KSUBB:RC_T41068_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
U133_X3P:HS.8377.0.S1_3P_AT Laminin, alpha 2 (merosin, congenital muscular dystrophy) hs
MOE430A:1426285_AT laminin, alpha 2 mm
MOUSE430A_2:1426285_AT laminin, alpha 2 mm
MOUSE430_2:1426285_AT laminin, alpha 2 mm
MU11KSUBA:U12147_S_AT laminin, alpha 2 mm
MG-U74AV2:92366_AT laminin, alpha 2 mm
RG-U34A:S72407_AT laminin, alpha 2 (predicted) rn
RG-U34C:RC_AI104456_AT laminin, alpha 2 (predicted) rn
RAE230A:1390404_AT laminin, alpha 2 (predicted) rn
RAT230_2:1390404_AT laminin, alpha 2 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7155 cell adhesion inferred from electronic annotation QuickGO AmiGO
30155 regulation of cell adhesion inferred from electronic annotation QuickGO AmiGO
30334 regulation of cell migration inferred from electronic annotation QuickGO AmiGO
45995 regulation of embryonic development inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5578 extracellular matrix (sensu Metazoa) inferred from electronic annotation QuickGO AmiGO
5606 laminin-1 inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5102 receptor binding inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB03385 Hypothetical protein T22A3.8 [Caenorhabditis elegans] emb|CAA15432.3| Hypothetical protein T22A3.8 [Caenorhabditis elegans] ref|NP_492775.2| LAMinin related. See also lmb- family member (lam-3) [Caenorhabditis elegans] gb|AAC26793.1| laminin alpha chain [Caenorhabditis elegans] 0.0
blast CAE56591 Hypothetical protein CBG24338 [Caenorhabditis briggsae] 0.0
blast AAB18388 laminin alpha 2 chain [Homo sapiens] 0.0
blast CAB03385 Hypothetical protein T22A3.8 [Caenorhabditis elegans] emb|CAA15432.3| Hypothetical protein T22A3.8 [Caenorhabditis elegans] ref|NP_492775.2| LAMinin related. See also lmb- family member (lam-3) [Caenorhabditis elegans] gb|AAC26793.1| laminin alpha chain [Caenorhabditis elegans] 1.0E-153
blast T33466 hypothetical protein C43H8.3 - Caenorhabditis elegans 1.0E-153
Protein Domains
Database ID Description E-Value
Pfam IPR008211 EMBL-EBI Laminin, N-terminal 1.6E-91
Pfam IPR000034 EMBL-EBI Laminin B 4.5E-20
Pfam IPR000034 EMBL-EBI Laminin B 1.9E-5
Pfam IPR010307 EMBL-EBI Laminin II 6.1E-56
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 1.4E-18
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 3.1E-21
Pfam IPR001791 EMBL-EBI Laminin G 1.6E-16
Pfam IPR001791 EMBL-EBI Laminin G 1.3E-10
Pfam IPR001791 EMBL-EBI Laminin G 1.7E-12
Pfam IPR009254 EMBL-EBI Laminin I 5.0E-106
Pfam IPR001791 EMBL-EBI Laminin G 1.7E-12
Sequence
Target Sequence 
>C. ELEGANS:182257_AT
gcgaaacatctctaggatctctccaaggagctgttagatacggtctatcaaagtcatcac
atagtagaatcaattttgaagctccatatccaaatatcacagatttcaccgtcaaactat
ccctccaaaccgaaacttcaaatggaatgatttgggcttgggccaactacaaaaactaca
ctcgctacattttccttgatataattgatggattcgcaacacttgaagtaaaaggacata
aacaaccaaaaattctgaaacatttgggaaatcgtataaatgatggacaatggcatgaca
ttactgttgaaaagaagaatcgatctttgaaattaataattgattcacttggaccagttg
aaatgacagattgtccaacaccaaaagtaatgaaaaagagagtatatgtgggtggagtca
tctcaagacacagaagacaatttggattgacaactcctggattagatggatgtattagag
attttgagatgaacaatagaatattcaataacctggatgaaccatcaacttccaagaatg
taatgccatgcgccaaagca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGAAACATCTCTAGGATCTCTCCA 550 289 251 Antisense
TCCAAGGAGCTGTTAGATACGGTCT 327 585 272 Antisense
ATACGGTCTATCAAAGTCATCACAT 438 23 288 Antisense
GAAGCTCCATATCCAAATATCACAG 357 343 328 Antisense
AGATTTCACCGTCAAACTATCCCTC 361 63 351 Antisense
TCCCTCCAAACCGAAACTTCAAATG 371 593 370 Antisense
GATTTGGGCTTGGGCCAACTACAAA 72 425 399 Antisense
ACTACAAAAACTACACTCGCTACAT 124 101 416 Antisense
ACACTCGCTACATTTTCCTTGATAT 168 109 428 Antisense
GTGGGTGGAGTCATCTCAAGACACA 56 489 658 Antisense
GAATGTAATGCCATGCGCCAAAGCA 152 331 786 Antisense
Sequence Source Affymetrix Proprietary Database