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[182247_s_at]

Description W02A11.1 /REP_DB=WormBase Gene ID /WP=CE18988 /TR=O62365 /GB=CAB04894.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 89      4grp_high_8_8 > 38      6grp_total > 49      4grp_total > 41      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 228
1 2 3 4 range
A 277.7
(-1.9)
P 		372.9
(1.4)
P 		419.4
(3)
P 		245.3
(-3)
A 		174
B 243.7
(-3)
P 		471.6
(3)
P 		414.4
(1.5)
P 		300.6
(-1.5)
P 		228
C 303.5
(-3)
P 		449.5
(3)
P 		371.9
(-0.2)
P 		385.5
(0.4)
P 		146
avr 275
(-3) 		431.3
(3) 		401.9
(1.9) 		310.5
(-1.6) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 229
1 2 3 4 5 6 range
A 430.8
(3)
M 		204.1
(-3)
P 		305.8
(-0.3)
P 		214.3
(-2.7)
M 		243
(-2)
P 		376.4
(1.6)
P 		227
B 263.8
(-0.9)
P 		201.4
(-3)
P 		248.6
(-1.4)
A 		289
(-0)
P 		378.8
(3)
P 		306.6
(0.6)
P 		177
C 365.7
(1)
P 		264.2
(-2.3)
P 		315
(-0.6)
P 		242.9
(-3)
A 		426.2
(3)
P 		359.1
(0.8)
P 		183
avr 353.4
(3) 		223.2
(-3) 		289.8
(0.1) 		248.7
(-1.8) 		349.3
(2.8) 		347.4
(2.7) 		130
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 101.1
(2.3)
P 		81
(0.7)
P 		109.6
(3)
P 		35.9
(-3)
A 		74
B 77.7
(-1.4)
P 		116.5
(3)
P 		96
(0.7)
P 		64.1
(-3)
P 		52
C 100.5
(0.3)
P 		129.2
(3)
P 		129.6
(3)
P 		65
(-3)
P 		65
avr 93.1
(1) 		108.9
(2.7) 		111.7
(3) 		55
(-3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 79.4
(1)
M 		72.6
(-0.1)
P 		66.6
(-1)
P 		54.3
(-3)
M 		73.3
(0.1)
P 		91.6
(3)
P 		37
B 91.8
(-1)
P 		67
(-2.6)
P 		60.4
(-3)
A 		76.5
(-2)
P 		153.9
(3)
P 		114.3
(0.5)
P 		94
C 135.5
(2.8)
P 		80.6
(-0.9)
P 		80.2
(-0.9)
P 		48.9
(-3)
A 		137.9
(3)
P 		120.2
(1.8)
P 		89
avr 102.2
(1.1) 		73.4
(-1.7) 		69.1
(-2.1) 		59.9
(-3) 		121.7
(3) 		108.7
(1.7) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182247_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.402
Sequence Type Exemplar sequence
Representative Public ID W02A11.1 NCBI
Target Description W02A11.1 /REP_DB=WormBase Gene ID /WP=CE18988 /TR=O62365 /GB=CAB04894.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060828(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060828 NCBI Caenorhabditis elegans W02A11.1 (W02A11.1) mRNA, complete cds. 11/11 None
W02A11.1 ENSEMBL cdna:known chromosome:CEL140:I:12738150:12740826:-1 gene:W02A11.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000035454 6/11 Cross Hyb Matching Probes None
SNAP00000035444 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12738141-12740749(-) 99.47 100.0
Public Domain and Genome References
Gene Title
Ensembl W02A11.1
Entrez Gene 173142 Entrez gene
SwissProt O62365 EMBL-EBI
WormBase CE18988 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250147_AT expressed protein at
CANINE_2:CFAAFFX.27979.1.S1_AT similar to CG14544-PA cfa
DROSOPHILA_2:1634023_AT dm
DROSGENOME1:150644_AT dm
CHICKEN:GGAAFFX.7300.1.S1_AT similar to MGC53312 protein gga
CHICKEN:GGAAFFX.7300.2.S1_S_AT similar to MGC53312 protein gga
HG-U133_PLUS_2:221907_AT chromosome 14 open reading frame 172 hs
HG-U133A_2:221907_AT chromosome 14 open reading frame 172 hs
HG-U133A:221907_AT chromosome 14 open reading frame 172 hs
HU35KSUBC:RC_AA417760_AT chromosome 14 open reading frame 172 hs
U133_X3P:HS2.339834.2.S1_3P_S_AT chromosome 14 open reading frame 172 hs
U133_X3P:HS.81920.0.A1_3P_X_AT chromosome 14 open reading frame 172 hs
U133_X3P:HS.81920.0.A1_3P_S_AT chromosome 14 open reading frame 172 hs
U133_X3P:HS.81920.0.A1_3P_AT chromosome 14 open reading frame 172 hs
U133_X3P:221907_3P_AT chromosome 14 open reading frame 172 hs
HG-U133_PLUS_2:1554317_S_AT chromosome 14 open reading frame 172 hs
HG-U133A_2:52741_AT chromosome 14 open reading frame 172 hs
HG-U133_PLUS_2:52741_AT chromosome 14 open reading frame 172 hs
HG-U133A:52741_AT chromosome 14 open reading frame 172 hs
HG-U95B:52741_AT chromosome 14 open reading frame 172 hs
HG-U95E:77156_AT chromosome 14 open reading frame 172 hs
MG-U74CV2:165729_AT RIKEN cDNA 6720458F09 gene mm
MOE430B:1433962_AT RIKEN cDNA 6720458F09 gene mm
MOUSE430_2:1433962_AT RIKEN cDNA 6720458F09 gene mm
MOE430B:1447048_AT RIKEN cDNA 6720458F09 gene, mRNA (cDNA clone MGC:57040 IMAGE:6468754) mm
MOUSE430_2:1447048_AT RIKEN cDNA 6720458F09 gene, mRNA (cDNA clone MGC:57040 IMAGE:6468754) mm
RG-U34B:RC_AA851343_AT similar to RIKEN cDNA 6720458F09 gene rn
RAE230A:1374945_AT similar to RIKEN cDNA 6720458F09 gene rn
RAT230_2:1374945_AT similar to RIKEN cDNA 6720458F09 gene rn
YEAST_2:1770507_AT Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Sc
Protein Similarities
Method ID Description E-Value
blast CAB04894 Hypothetical protein W02A11.1 [Caenorhabditis elegans] emb|CAB04711.1| Hypothetical protein W02A11.1 [Caenorhabditis elegans] ref|NP_493229.1| W02A11.1 [Caenorhabditis elegans] 1.0E-177
blast CAE67991 Hypothetical protein CBG13601 [Caenorhabditis briggsae] 1.0E-156
blast AAH76660 MGC79558 protein [Xenopus tropicalis] ref|NP_001005009.1| MGC79558 protein [Xenopus tropicalis] 9.0E-67
Sequence
Target Sequence 
>C. ELEGANS:182247_S_AT
atccttgtcccatgcaattgcactgaccgttgcaccaaccggacatctgtatacacatga
tatcgacgaaacacgtactcataaagttttggaggaatttaaggttcatggcctaggatc
cgtgacgacggccgtcgttcgaaatgtctgcacctctggattccacgtggaaaacgctgc
cgacggggtattcctggatgtaccggcaccgtgggaggcaatcccattcgccgcaaaatc
aattttccgggctcgtggaggtcgtctagtgtcttttagtccgtgcctggaacaagtcca
acgtgcctgcctagccatggcggccgccggtttcgtgagcatcgagacgattgagctcgt
tcccgagcagaagaagatcgtcagtcaacaccggcagacgcttgaagagtttgaagagct
cggtgatgcttatccgaaggaacggaagagaaatgtcgatggaacaatcgttgagcaaag
cagctccgccattccacgtatcacaattgcaatcgtctatccatattctcagccaacaca
cactgggtacctgact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCCTTGTCCCATGCAATTGCACTG 611 37 366 Antisense
TTGCACCAACCGGACATCTGTATAC 344 701 395 Antisense
TTCATGGCCTAGGATCCGTGACGAC 710 687 470 Antisense
TGCCGACGGGGTATTCCTGGATGTA 382 581 543 Antisense
TGTACCGGCACCGTGGGAGGCAATC 651 555 564 Antisense
AATCCCATTCGCCGCAAAATCAATT 678 167 585 Antisense
GAGGTCGTCTAGTGTCTTTTAGTCC 629 399 623 Antisense
TCGAGACGATTGAGCTCGTTCCCGA 298 603 707 Antisense
GCCATTCCACGTATCACAATTGCAA 339 281 853 Antisense
TGCAATCGTCTATCCATATTCTCAG 552 575 873 Antisense
GCCAACACACACTGGGTACCTGACT 255 273 897 Antisense
Sequence Source Affymetrix Proprietary Database