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[182245_at]

Description ZC487.5 /REP_DB=WormBase Gene ID /WP=CE07607 /TR=Q23353 /GB=AAA92542.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 23      6grp_cgc > 99      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 176.3
(3)
A 		23.6
(-3)
A 		93.3
(-0.3)
A 		143.3
(1.7)
A 		153
B 178.7
(3)
A 		14.8
(-3)
A 		14
(-3)
A 		122.4
(0.9)
A 		165
C 134.5
(3)
A 		45
(-1.3)
A 		8.3
(-3)
A 		123.1
(2.5)
A 		126
avr 163.2
(3) 		27.8
(-3) 		38.5
(-2.5) 		129.6
(1.5) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 186
1 2 3 4 5 6 range
A 57
(-1.6)
A 		118.2
(1.9)
A 		31.5
(-3)
A 		92.9
(0.4)
A 		110.6
(1.4)
A 		138.3
(3)
A 		107
B 99.1
(-0.3)
A 		129.1
(0.6)
A 		16.5
(-3)
A 		18.8
(-2.9)
A 		202.7
(3)
A 		43.9
(-2.1)
A 		186
C 175.5
(3)
A 		69.4
(-1.9)
A 		95.3
(-0.7)
A 		45.7
(-3)
A 		133.4
(1.1)
A 		55.7
(-2.5)
A 		130
avr 110.5
(0.7) 		105.6
(0.4) 		47.8
(-3) 		52.5
(-2.7) 		148.9
(3) 		79.3
(-1.1) 		101
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 64.2
(3)
A 		5.1
(-3)
A 		24.4
(-1)
A 		21
(-1.4)
A 		59
B 57
(3)
A 		3.7
(-3)
A 		3.3
(-3)
A 		26.1
(-0.5)
A 		54
C 44.5
(3)
A 		12.9
(-1.6)
A 		2.9
(-3)
A 		20.8
(-0.4)
A 		42
avr 55.2
(3) 		7.2
(-3) 		10.2
(-2.6) 		22.6
(-1.1) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 10.5
(-2.4)
A 		42
(3)
A 		6.9
(-3)
A 		23.6
(-0.1)
A 		33.4
(1.5)
A 		33.7
(1.6)
A 		35
B 34.5
(-0.7)
A 		43
(-0)
A 		4
(-3)
A 		5
(-2.9)
A 		82.3
(3)
A 		16.4
(-2)
A 		78
C 65
(3)
A 		21.2
(-1.7)
A 		24.3
(-1.4)
A 		9.2
(-3)
A 		43.2
(0.7)
A 		18.6
(-2)
A 		56
avr 36.7
(0.6) 		35.4
(0.4) 		11.7
(-3) 		12.6
(-2.9) 		53
(3) 		22.9
(-1.4) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182245_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12351
Sequence Type Exemplar sequence
Representative Public ID ZC487.5 NCBI
Target Description ZC487.5 /REP_DB=WormBase Gene ID /WP=CE07607 /TR=Q23353 /GB=AAA92542.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072306(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072306 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-8) (grl-8) mRNA, complete cds. 11/11 None
SNAP00000039163 ENSEMBL cdna:SNAP chromosome:CEL140:V:6734542:6735670:-1 11/11 None
GENEFINDER00000039164 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6734542:6735670:-1 11/11 None
ZC487.5 ENSEMBL cdna:known chromosome:CEL140:V:6734542:6735670:-1 gene:ZC487.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6734542-6735671(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-8
Ensembl ZC487.5
Entrez Gene 191191 Entrez gene
SwissProt Q23353 EMBL-EBI
WormBase CE07607 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA92542 Ground-like (grd related) protein 8 [Caenorhabditis elegans] ref|NP_504707.1| GRound-Like (grd related) family member (grl-8) [Caenorhabditis elegans] 1.0E-101
blast CAE71942 Hypothetical protein CBG19006 [Caenorhabditis briggsae] 2.0E-59
blast CAE56051 Hypothetical protein CBG23622 [Caenorhabditis briggsae] 2.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 3.3E-31
Sequence
Target Sequence 
>C. ELEGANS:182245_AT
gagccaccgactgtggatgcatcaccatcattaacatctgaaaaacgaacagtcgccatg
gaggtgacgtcatcagcacatcacctgcttaggcccaagactgaatcggattctgaagaa
gaacaaccaaaacctacaagaaatagagtcagattgcattcgcacaattcaaaagtttct
aaagtgtctagaactcgagaagttgaagttttgagtgaaaaatgtaatgatgataaattg
gaacagatcatgcaagatgccatgactccaagtttaagcacctcaaaaatggtgatttct
gagcgagcgactcgagatttcggtgcaaattttgatgtgatctgtgcaagaggtcacttt
tcatattatgtggaagcggcctcatattgtgaagtaactatgaacgatgtcacttgtctt
gcttacaaacccggaccccagacggatgatggttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCCACCGACTGTGGATGCATCAC 470 385 106 Antisense
ATGCATCACCATCATTAACATCTGA 281 43 122 Antisense
GAACAGTCGCCATGGAGGTGACGTC 397 351 152 Antisense
ATCACCTGCTTAGGCCCAAGACTGA 43 27 185 Antisense
GAGTCAGATTGCATTCGCACAATTC 284 399 251 Antisense
GCAAGATGCCATGACTCCAAGTTTA 361 321 357 Antisense
AATGGTGATTTCTGAGCGAGCGACT 446 163 393 Antisense
GCGAGCGACTCGAGATTTCGGTGCA 71 291 408 Antisense
GTGGAAGCGGCCTCATATTGTGAAG 539 491 475 Antisense
GAACGATGTCACTTGTCTTGCTTAC 58 347 507 Antisense
CCGGACCCCAGACGGATGATGGTTC 382 263 536 Antisense
Sequence Source Affymetrix Proprietary Database