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[182227_s_at]

Description K12F2.2A /REP_DB=WormBase Gene ID /WP=CE21070 /TR=Q21441 /GB=CAB01577.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 35      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 71      6grp_total > 35      4grp_total > 22      6grp_highest_sub > Cu      6grp_cgc > 34      6grp_high1_sub > 02      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 302
1 2 3 4 range
A 472.5
(2.3)
P 		367.3
(-0.5)
P 		270
(-3)
P 		499.5
(3)
P 		230
B 518.9
(3)
P 		347.9
(-2.9)
P 		344.3
(-3)
P 		452.9
(0.7)
P 		175
C 487.4
(1.2)
P 		309.3
(-2.6)
P 		292.2
(-3)
P 		572
(3)
P 		280
avr 492.9
(2.6) 		341.5
(-1.9) 		302.2
(-3) 		508.1
(3) 		206

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 317
(-0.8)
P 		441.3
(3)
P 		305.1
(-1.2)
P 		331.5
(-0.4)
P 		246.3
(-3)
P 		336.1
(-0.2)
P 		195
B 379.1
(3)
P 		329.5
(0)
P 		281.1
(-2.9)
P 		278.9
(-3)
P 		284.4
(-2.7)
P 		337.2
(0.5)
P 		100
C 465.4
(2.1)
P 		481.8
(2.5)
P 		500.1
(3)
P 		266.4
(-3)
P 		265.2
(-3)
P 		361.2
(-0.5)
P 		235
avr 387.2
(1.8) 		417.5
(3) 		362.1
(0.8) 		292.3
(-1.9) 		265.3
(-3) 		344.8
(0.1) 		152
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 172.1
(3)
P 		79.7
(-2.5)
P 		70.6
(-3)
P 		73.1
(-2.9)
P 		102
B 165.5
(3)
P 		85.9
(-2.6)
P 		79.8
(-3)
P 		96.5
(-1.8)
P 		86
C 161.3
(3)
P 		88.9
(-3)
P 		101.8
(-1.9)
P 		96.5
(-2.4)
P 		72
avr 166.3
(3) 		84.8
(-2.9) 		84.1
(-3) 		88.7
(-2.7) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 119
1 2 3 4 5 6 range
A 58.4
(-3)
P 		157
(3)
P 		66.4
(-2.5)
P 		84.1
(-1.4)
P 		74.3
(-2)
P 		81.8
(-1.6)
P 		99
B 131.9
(3)
P 		109.7
(0.9)
P 		68.2
(-3)
P 		73.9
(-2.5)
P 		115.5
(1.5)
P 		125.7
(2.4)
P 		64
C 172.5
(3)
P 		147
(1.7)
P 		127.3
(0.7)
P 		53.6
(-3)
P 		85.8
(-1.4)
P 		120.9
(0.4)
P 		119
avr 120.9
(1.5) 		137.9
(3) 		87.3
(-1.5) 		70.5
(-3) 		91.9
(-1.1) 		109.5
(0.5) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182227_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14552
Sequence Type Exemplar sequence
Representative Public ID K12F2.2A NCBI
Target Description K12F2.2A /REP_DB=WormBase Gene ID /WP=CE21070 /TR=Q21441 /GB=CAB01577.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073662(11), NM_073663(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073662 NCBI Caenorhabditis elegans Variable ABnormal morphology family member (vab-8) (vab-8) mRNA, complete cds. 11/11 A 
NM_073663 NCBI Caenorhabditis elegans Variable ABnormal morphology family member (vab-8) (vab-8) mRNA, complete cds. 11/11 None
SNAP00000012837 ENSEMBL cdna:SNAP chromosome:CEL140:V:12226349:12228329:1 11/11 None
GENEFINDER00000012840 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12224780:12228329:1 11/11 None
K12F2.2a ENSEMBL cdna:known chromosome:CEL140:V:12215915:12228508:1 gene:K12F2.2 11/11 None
K12F2.2b ENSEMBL cdna:known chromosome:CEL140:V:12225712:12228329:1 gene:K12F2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12224779-12228329(+) 100.0 100.0
Public Domain and Genome References
Ensembl K12F2.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD36504 Hypothetical protein K12F2.2a [Caenorhabditis elegans] emb|CAB01577.2| Hypothetical protein K12F2.2a [Caenorhabditis elegans] ref|NP_506063.2| Variable ABnormal morphology family member (vab-8) [Caenorhabditis elegans] gb|AAF17300.1| VAB-8L [Caenorhabditis elegans] 0.0
blast CAB54275 Hypothetical protein K12F2.2b [Caenorhabditis elegans] ref|NP_506064.1| Variable ABnormal morphology family member (vab-8) [Caenorhabditis elegans] gb|AAF17301.1| VAB-8S [Caenorhabditis elegans] 0.0
blast CAE75420 Hypothetical protein CBG23411 [Caenorhabditis briggsae] 0.0
Sequence
Target Sequence 
>C. ELEGANS:182227_S_AT
cagtcaatgggcgacgtgcttctgcagaaagagaaagaaaatcaaataaggcctcaagaa
attcctgttccaaagaaagaaagatcagtggaagtaaagaagaactccaatggagaagtc
cttatgctcaaatgacgtcaccgaaatcatatggtggacctggtacctcatcatcaggaa
gagggtctagtgcccctggctctgattttgaaactccagtggtttctacaactgaaaaat
ctgcaaatggaacaattccgagatcgaagagacaaagctattcggcttcatctggatacg
agtcagcaaatgattatcatatttattcaacgacaaacaagaaacctcacattcttgaca
aaaaacgaaacgaagaaaaactttcacttgtacggcaggccgatgagattcgtcatcgac
agtggcaactgaaaaaagaactcgaagaagcgaaaagagctattggacaggaagatgatg
caaaaatgatcgcgaattcaagtgaccagagactaaacgggttaagtcgaacgacaatga
ttgatgcgatgcttcaagaaaatcgaattcttgagaagcggctagttgcatgtcgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGTCAATGGGCGACGTGCTTCTGC 139 207 1238 Antisense
GGCCTCAAGAAATTCCTGTTCCAAA 332 543 1287 Antisense
TATGGTGGACCTGGTACCTCATCAT 104 655 1387 Antisense
AGGAAGAGGGTCTAGTGCCCCTGGC 81 55 1413 Antisense
CCCCTGGCTCTGATTTTGAAACTCC 239 261 1430 Antisense
AAACTCCAGTGGTTTCTACAACTGA 324 127 1448 Antisense
GCTATTCGGCTTCATCTGGATACGA 490 303 1514 Antisense
GAAAAACTTTCACTTGTACGGCAGG 141 327 1612 Antisense
ACGGCAGGCCGATGAGATTCGTCAT 137 97 1629 Antisense
ATTCGTCATCGACAGTGGCAACTGA 516 9 1645 Antisense
GAGAAGCGGCTAGTTGCATGTCGAA 133 393 1810 Antisense
Sequence Source Affymetrix Proprietary Database