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[182223_at]

Description F28B3.5 /REP_DB=WormBase Gene ID /WP=CE09743 /TR=O01783 /GB=AAK21376.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 0      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_total > 08      4grp_total > 53      6grp_cgc > 20      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 217.8
(3)
P 		158.1
(-2.2)
P 		148.6
(-3)
P 		165.5
(-1.5)
P 		69
B 178.3
(0.7)
P 		220
(2.6)
P 		228.3
(3)
P 		98.9
(-3)
P 		129
C 220.4
(2.1)
P 		231.6
(3)
P 		156.3
(-3)
P 		188.5
(-0.4)
P 		75
avr 205.5
(3) 		203.2
(2.7) 		177.7
(-0.1) 		151
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 188
1 2 3 4 5 6 range
A 277.3
(3)
P 		156
(-1.1)
P 		170
(-0.6)
P 		144.8
(-1.5)
P 		137.8
(-1.7)
P 		98.9
(-3)
P 		178
B 158
(0)
P 		134.5
(-1.6)
P 		165.6
(0.6)
P 		200.8
(3)
P 		114.1
(-3)
P 		183.3
(1.8)
P 		87
C 162.5
(3)
P 		153.5
(2.3)
P 		141.4
(1.3)
P 		89
(-3)
M 		99.7
(-2.1)
P 		106.3
(-1.6)
P 		74
avr 199.3
(3) 		148
(-0.7) 		159
(0.1) 		144.9
(-1) 		117.2
(-3) 		129.5
(-2.1) 		82
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 79.3
(3)
P 		34.3
(-1.9)
P 		38.8
(-1.4)
P 		24.2
(-3)
P 		55
B 56.9
(3)
P 		54.3
(2.6)
P 		52.9
(2.3)
P 		21.1
(-3)
P 		36
C 73
(3)
P 		66.6
(2.1)
P 		54.5
(0.3)
P 		31.8
(-3)
P 		41
avr 69.7
(3) 		51.7
(0.5) 		48.7
(0.1) 		25.7
(-3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 51.1
(2.2)
P 		55.5
(3)
P 		37
(-0.5)
P 		36.7
(-0.6)
P 		41.6
(0.3)
P 		24.1
(-3)
P 		31
B 55
(0.2)
P 		44.8
(-2)
P 		40.2
(-3)
P 		53.2
(-0.2)
P 		46.3
(-1.7)
P 		68.3
(3)
P 		28
C 60.2
(3)
P 		46.8
(1.1)
P 		36
(-0.4)
P 		17.9
(-3)
M 		32.3
(-1)
P 		35.6
(-0.5)
P 		42
avr 55.4
(3) 		49
(1) 		37.7
(-2.4) 		35.9
(-3) 		40.1
(-1.7) 		42.7
(-0.9) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182223_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.632
Sequence Type Exemplar sequence
Representative Public ID F28B3.5 NCBI
Target Description F28B3.5 /REP_DB=WormBase Gene ID /WP=CE09743 /TR=O01783 /GB=AAK21376.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059078(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059078 NCBI Caenorhabditis elegans ACyLtransferase-like family member (acl-11) (acl-11) mRNA, complete cds. 11/11 None
GENEFINDER00000020911 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4926734:4931819:1 11/11 None
SNAP00000020902 ENSEMBL cdna:SNAP chromosome:CEL140:I:4929100:4931819:1 11/11 None
F28B3.9 ENSEMBL cdna:known chromosome:CEL140:I:4929100:4932148:1 gene:F28B3.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4926436-4931819(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol acl-11
Ensembl F28B3.9
Entrez Gene 185044 Entrez gene
SwissProt Q95R12 EMBL-EBI
WormBase CE37364 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGA.12634.1.S1_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) gga
HU35KSUBA:RC_AA207114_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HU35KSUBB:RC_AA056538_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HU35KSUBC:RC_AA281331_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HG-U133A:218096_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HG-U133_PLUS_2:218096_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HG-U133A_2:218096_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
U133_X3P:G8922941_3P_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HG-U95B:45739_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HU35KSUBC:RC_N54425_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
U133_X3P:HS.281895.0.S1_3P_X_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
U133_X3P:HS.281895.0.S1_3P_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HG-U133_PLUS_2:232007_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HG-U133B:232007_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HG-U95B:45994_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
HG-U95E:67123_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) hs
MOUSE430_2:1434287_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) mm
MOE430A:1434287_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) mm
MOUSE430A_2:1434287_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) mm
MU11KSUBA:C80598_RC_G_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) mm
MU11KSUBA:C80598_RC_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) mm
MG-U74AV2:103924_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) mm
MG-U74BV2:111222_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) mm
MG-U74BV2:163212_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) mm
MOE430B:1453257_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) mm
MOUSE430_2:1453257_AT 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) mm
MG-U74CV2:131875_AT DNA segment, Chr 8, ERATO Doi 319, expressed, mRNA (cDNA clone MGC:25566 IMAGE:3982362) mm
MU19KSUBB:TC27553_AT DNA segment, Chr 8, ERATO Doi 319, expressed, mRNA (cDNA clone MGC:25566 IMAGE:3982362) mm
MU19KSUBC:TC41852_AT DNA segment, Chr 8, ERATO Doi 319, expressed, mRNA (cDNA clone MGC:25566 IMAGE:3982362) mm
RAE230B:1395940_AT similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RAT230_2:1395940_AT similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RAE230A:1373169_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RAT230_2:1373169_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RAE230B:1378284_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RAT230_2:1378284_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RAE230B:1395006_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RAT230_2:1395006_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RAE230B:1396126_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RAT230_2:1396126_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RG-U34B:RC_AA956157_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RG-U34C:RC_AI100841_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
RG-U34C:RC_AI175416_AT Similar to 1-acylglycerolphosphate acyltransferase-epsilon (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8415 acyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK93853 Acyltransferase-like protein 11 [Caenorhabditis elegans] ref|NP_491479.2| ACyLtransferase-like family member (acl-11) [Caenorhabditis elegans] 0.0
blast T34057 hypothetical protein F28B3.5 - Caenorhabditis elegans 0.0
blast AAK93851 Hypothetical protein F28B3.5a [Caenorhabditis elegans] ref|NP_491478.1| F28B3.5a [Caenorhabditis elegans] 0.0
blast CAE73115 Hypothetical protein CBG20496 [Caenorhabditis briggsae] 0.0
blast CAE73116 Hypothetical protein CBG20497 [Caenorhabditis briggsae] 1.0E-175
Protein Domains
Database ID Description E-Value
Pfam IPR002123 EMBL-EBI Phospholipid/glycerol acyltransferase 6.5E-6
Pfam IPR002123 EMBL-EBI Phospholipid/glycerol acyltransferase 6.5E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491479.2 2 20-42,347-364
F28B3.9 2 20-42,347-364
GENEFINDER00000020911 14 5-27,40-62,74-93,108-130,143-161,171-193,249-271,286-308,315-337,344-366,387-409,431-450,486-508,821-838
SNAP00000020902 2 20-42,382-399
Sequence
Target Sequence 
>C. ELEGANS:182223_AT
gaaaagtggccgtcaaccaatgcaaaatgtgttgtgtccgcgcagtggaggattacaatt
ggcactcgacaatttgtcgacacttgacgctatctatgatgttactgtaatgtatggaca
aatgaggtttgatttgggattgaatttaacaattcattataatctaattattatctacag
aatggcggaacgtcgtggattggcgcctggaatgtttgatttttgttgtggatcacaaca
attcaaacaacttcatattcatcttgataggattccaattgatgaggttcccaaggcgaa
attggagctccgcacctggacgattgagcgattcacgaaaaaggaaagaattattgatga
attctattcggagaaaccatccaccggaagtgcactcccgtgcgtgccaatttcacagac
tctcccatcgacactcttcttctcagctgctcttcttgctccgttcttcagcagaacaat
tggaagaatctacttgttaacgatcgcatcttcaccattgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAAGTGGCCGTCAACCAATGCAA 133 355 2214 Antisense
CAAAATGTGTTGTGTCCGCGCAGTG 87 191 2236 Antisense
TCGACACTTGACGCTATCTATGATG 418 601 2290 Antisense
ATGGCGGAACGTCGTGGATTGGCGC 409 53 2395 Antisense
GGATTGGCGCCTGGAATGTTTGATT 688 507 2410 Antisense
AAGGCGAAATTGGAGCTCCGCACCT 21 161 2506 Antisense
GCACCTGGACGATTGAGCGATTCAC 427 315 2525 Antisense
GAGAAACCATCCACCGGAAGTGCAC 472 389 2584 Antisense
CCGTGCGTGCCAATTTCACAGACTC 413 265 2611 Antisense
TTCTTGCTCCGTTCTTCAGCAGAAC 316 689 2666 Antisense
TTAACGATCGCATCTTCACCATTGC 411 683 2710 Antisense
Sequence Source Affymetrix Proprietary Database