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[182221_at]

Description ZK154.4 /REP_DB=WormBase Gene ID /WP=CE28176 /TR=Q94313 /GB=AAB09094.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 40      4grp_highest_8_8 > 0      4grp_high_8_8 > 44      6grp_highest_nonorm > 71      6grp_total > 42      4grp_total > 51      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 328
(-3)
P 		401.9
(3)
P 		340.2
(-2)
P 		335.7
(-2.4)
P 		74
B 368.2
(-1.1)
P 		475.3
(3)
P 		438.5
(1.6)
P 		319.2
(-3)
P 		156
C 422.1
(1.7)
P 		446.5
(3)
P 		401.7
(0.5)
P 		337.3
(-3)
P 		109
avr 372.8
(-0.7) 		441.2
(3) 		393.5
(0.4) 		330.7
(-3) 		111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 286
1 2 3 4 5 6 range
A 390.6
(1.9)
P 		333.6
(0.2)
P 		425.3
(3)
P 		340.2
(0.4)
P 		340.5
(0.4)
P 		231.5
(-3)
P 		194
B 344.1
(-1.2)
P 		384.7
(-0.2)
P 		517.8
(3)
P 		349.9
(-1)
P 		359.2
(-0.8)
P 		268.6
(-3)
P 		249
C 326.6
(-0.8)
P 		267.4
(-3)
P 		393.5
(1.7)
P 		428.1
(3)
P 		314.6
(-1.2)
P 		290.3
(-2.1)
P 		161
avr 353.8
(-0) 		328.6
(-0.9) 		445.5
(3) 		372.7
(0.6) 		338.1
(-0.5) 		263.5
(-3) 		182
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 119.4
(3)
P 		87.2
(0.3)
P 		88.9
(0.4)
P 		49.1
(-3)
P 		70
B 117.4
(3)
P 		117.4
(3)
P 		101.6
(1.1)
P 		68
(-3)
P 		49
C 139.7
(3)
P 		128.4
(2.2)
P 		140
(3)
P 		56.9
(-3)
P 		83
avr 125.5
(3) 		111
(1.7) 		110.2
(1.6) 		58
(-3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 72
(-1.5)
P 		118.6
(3)
P 		92.6
(0.5)
P 		86.3
(-0.1)
P 		102.7
(1.5)
P 		56.4
(-3)
P 		62
B 119.7
(0)
P 		128.1
(1)
P 		125.7
(0.7)
P 		92.7
(-3)
P 		145.9
(3)
P 		100.2
(-2.2)
P 		53
C 121.1
(3)
P 		81.6
(-3)
P 		100.2
(-0.2)
P 		86.1
(-2.3)
P 		101.8
(0.1)
P 		97.2
(-0.6)
P 		40
avr 104.3
(0.7) 		109.4
(1.6) 		106.2
(1) 		88.4
(-2.3) 		116.8
(3) 		84.6
(-3) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182221_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16148
Sequence Type Exemplar sequence
Representative Public ID ZK154.4 NCBI
Target Description ZK154.4 /REP_DB=WormBase Gene ID /WP=CE28176 /TR=Q94313 /GB=AAB09094.2 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076914(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076914 NCBI Caenorhabditis elegans ZK154.4 (ZK154.4) mRNA, complete cds. 11/11 None
SNAP00000014392 ENSEMBL cdna:SNAP chromosome:CEL140:X:7779729:7782349:-1 11/11 None
GENEFINDER00000014401 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7781019:7792769:-1 11/11 None
ZK154.4 ENSEMBL cdna:known chromosome:CEL140:X:7776988:7782349:-1 gene:ZK154.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7781017-7782348(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK154.4
Entrez Gene 181037 Entrez gene
SwissProt Q94313 EMBL-EBI
WormBase CE28176 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB09095 Hypothetical protein ZK154.5 [Caenorhabditis elegans] ref|NP_509316.1| ZK154.5 [Caenorhabditis elegans] 0.0
blast AAB09094 Hypothetical protein ZK154.4 [Caenorhabditis elegans] ref|NP_509315.1| ZK154.4 [Caenorhabditis elegans] 9.0E-86
blast AAB09094 Hypothetical protein ZK154.4 [Caenorhabditis elegans] ref|NP_509315.1| ZK154.4 [Caenorhabditis elegans] 7.0E-79
blast AAB09094 Hypothetical protein ZK154.4 [Caenorhabditis elegans] ref|NP_509315.1| ZK154.4 [Caenorhabditis elegans] 4.0E-63
blast CAE70275 Hypothetical protein CBG16787 [Caenorhabditis briggsae] 1.0E-60
blast CAE70275 Hypothetical protein CBG16787 [Caenorhabditis briggsae] 4.0E-57
blast CAB03471 Hypothetical protein W02D9.4 [Caenorhabditis elegans] ref|NP_493179.1| W02D9.4 [Caenorhabditis elegans] 6.0E-48
Sequence
Target Sequence 
>C. ELEGANS:182221_AT
aattgttcgggcagtatacgtgtcgacgatgcgtctccttcaattgattattgcttttat
tcggaatttgtgcaatgggaatcgcaacaaaaagcgcatggatgtggatgacttgccgtt
tgaggtggtcgtcgacaacggtcgtacagaaaggaaaggtgaagaacgagcatcactgct
tcaaaatgaagtcaccgattcgtggggagacgatgactgggataaaaaggaagaagtgaa
cgacaaaattgagcaatggagaaacagtaatgctcagaaaccggacacccacaccaaacc
agatgacgacttgtttagcacactagagccaactattaccagcgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTGTTCGGGCAGTATACGTGTCG 185 179 18 Antisense
ATACGTGTCGACGATGCGTCTCCTT 439 23 33 Antisense
CGATGCGTCTCCTTCAATTGATTAT 156 249 44 Antisense
GACTTGCCGTTTGAGGTGGTCGTCG 567 1 127 Antisense
TGGTCGTCGACAACGGTCGTACAGA 382 551 143 Antisense
GAAGAACGAGCATCACTGCTTCAAA 251 341 178 Antisense
GAAGTCACCGATTCGTGGGGAGACG 293 337 205 Antisense
AGTAATGCTCAGAAACCGGACACCC 134 63 283 Antisense
CCCACACCAAACCAGATGACGACTT 53 259 305 Antisense
GACGACTTGTTTAGCACACTAGAGC 353 379 322 Antisense
ACTAGAGCCAACTATTACCAGCGCC 692 101 339 Antisense
Sequence Source Affymetrix Proprietary Database