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[182218_s_at]

Description W07E6.2 /REP_DB=WormBase Gene ID /WP=CE17294 /TR=Q9TYV3 /GB=AAC78176.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 58      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 82      6grp_total > 49      4grp_total > 03      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 754
1 2 3 4 range
A 699.7
(-2.1)
P 		619.9
(-3)
P 		718.7
(-1.9)
P 		1159.2
(3)
P 		539
B 891.8
(-1.5)
P 		843.9
(-2)
P 		732.1
(-3)
P 		1373.7
(3)
P 		642
C 723.3
(-3)
P 		790.6
(-2.4)
P 		730.3
(-2.9)
P 		1351.1
(3)
P 		628
avr 771.6
(-2.5) 		751.5
(-2.7) 		727
(-3) 		1294.7
(3) 		568

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 326
1 2 3 4 5 6 range
A 542.4
(3)
P 		328.6
(-1.9)
P 		279.6
(-3)
P 		342
(-1.6)
P 		360.4
(-1.2)
P 		532.8
(2.8)
P 		263
B 513.2
(2.2)
P 		397.9
(0.1)
P 		290.5
(-1.9)
P 		230.2
(-3)
P 		387.8
(-0.1)
P 		556.3
(3)
P 		326
C 424.2
(1.2)
P 		403.2
(0.6)
P 		272.6
(-3)
P 		299.9
(-2.2)
P 		406.3
(0.7)
P 		490.7
(3)
P 		218
avr 493.3
(2.2) 		376.6
(-0.7) 		280.9
(-3) 		290.7
(-2.8) 		384.8
(-0.5) 		526.6
(3) 		246
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 254.8
(3)
P 		134.6
(-3)
P 		187.9
(-0.3)
P 		169.7
(-1.2)
P 		120
B 284.4
(2.6)
P 		208.4
(-1.1)
P 		169.6
(-3)
P 		292.7
(3)
P 		123
C 239.4
(-0.3)
P 		227.3
(-3)
P 		254.5
(3)
P 		227.8
(-2.9)
P 		27
avr 259.5
(3) 		190.1
(-3) 		204
(-1.8) 		230.1
(0.5) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 147
1 2 3 4 5 6 range
A 100
(0.4)
P 		116.9
(1.9)
P 		60.9
(-3)
P 		86.7
(-0.7)
P 		108.8
(1.2)
P 		129.7
(3)
P 		69
B 178.6
(1.8)
P 		132.4
(-0.1)
P 		70.5
(-2.6)
P 		61
(-3)
P 		157.6
(1)
P 		207.4
(3)
P 		146
C 157.2
(2.6)
P 		123
(0.6)
P 		69.4
(-2.5)
P 		60.3
(-3)
P 		131.5
(1.1)
P 		164.3
(3)
P 		104
avr 145.3
(1.7) 		124.1
(0.4) 		66.9
(-3) 		69.3
(-2.9) 		132.6
(0.9) 		167.1
(3) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182218_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5611
Sequence Type Exemplar sequence
Representative Public ID W07E6.2 NCBI
Target Description W07E6.2 /REP_DB=WormBase Gene ID /WP=CE17294 /TR=Q9TYV3 /GB=AAC78176.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061344(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061344 NCBI Caenorhabditis elegans W07E6.2 (W07E6.2) mRNA, complete cds. 11/11 None
SNAP00000002746 ENSEMBL cdna:SNAP chromosome:CEL140:II:480824:489419:-1 11/11 None
GENEFINDER00000002752 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:480824:483085:-1 11/11 None
W07E6.2.2 ENSEMBL cdna:known chromosome:CEL140:II:480727:484392:-1 gene:W07E6.2 11/11 None
W07E6.2.1 ENSEMBL cdna:known chromosome:CEL140:II:480727:484385:-1 gene:W07E6.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:480823-484377(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W07E6.2
Entrez Gene 173439 Entrez gene
SwissProt Q9TYV3 EMBL-EBI
WormBase CE17294 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:248326_AT WD-40 repeat family protein / notchless protein, putative at
CANINE_2:CFAAFFX.27994.1.S1_AT similar to Notchless homolog 1 (WD-repeat protein HUSSY-07) cfa
DROSGENOME1:143952_AT Notchless dm
DROSOPHILA_2:1632721_AT Notchless dm
CHICKEN:GGAAFFX.2912.1.S1_S_AT similar to Nle-pending-prov protein gga
HG-U95AV2:37937_AT notchless homolog 1 (Drosophila) hs
HG-U133A:203867_S_AT notchless homolog 1 (Drosophila) hs
HG-U133_PLUS_2:203867_S_AT notchless homolog 1 (Drosophila) hs
HG-FOCUS:203867_S_AT notchless homolog 1 (Drosophila) hs
HG-U133A_2:203867_S_AT notchless homolog 1 (Drosophila) hs
HU35KSUBA:AA203300_AT notchless homolog 1 (Drosophila) hs
HU35KSUBB:RC_W73253_S_AT notchless homolog 1 (Drosophila) hs
U133_X3P:G8922427_3P_A_AT notchless homolog 1 (Drosophila) hs
U133_X3P:HS.85570.0.S2_3P_AT notchless homolog 1 (Drosophila) hs
HG-U133A_2:203866_AT notchless homolog 1 (Drosophila) hs
HG-U133_PLUS_2:203866_AT notchless homolog 1 (Drosophila) hs
HG-U133A:203866_AT notchless homolog 1 (Drosophila) hs
MOUSE430_2:1424731_AT notchless homolog 1 (Drosophila) mm
MOE430A:1424731_AT notchless homolog 1 (Drosophila) mm
MOUSE430A_2:1424731_AT notchless homolog 1 (Drosophila) mm
MG-U74BV2:112477_AT notchless homolog 1 (Drosophila) mm
MU19KSUBA:TC21824_AT Notchless homolog 1 (Drosophila) (Nle1), mRNA mm
RG-U34B:RC_AI059154_AT similar to Notchless gene homolog; similar to hypothetical protein similar to beta-transducin family (predicted) rn
RAE230A:1389765_AT similar to Notchless gene homolog; similar to hypothetical protein similar to beta-transducin family (predicted) rn
RAT230_2:1389765_AT similar to Notchless gene homolog; similar to hypothetical protein similar to beta-transducin family (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5834 heterotrimeric G-protein complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC78176 Hypothetical protein W07E6.2 [Caenorhabditis elegans] ref|NP_493745.1| W07E6.2 [Caenorhabditis elegans] 0.0
blast CAE62878 Hypothetical protein CBG07064 [Caenorhabditis briggsae] 0.0
blast NP_060566 Notchless gene homolog isoform a [Homo sapiens] 1.0E-166
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.2E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 6.6E-11
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.1E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 8.1E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.1E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.1E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.8E-11
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.9E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.5E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 8.1E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.1E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.1E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.8E-11
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.9E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.2E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 6.6E-11
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.1E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 8.1E-10
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.1E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 3.1E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.8E-11
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 4.9E-9
Sequence
Target Sequence 
>C. ELEGANS:182218_S_AT
ggacacgctcactggattaacactttggcactgaatacagattatgcgttgagaacctcg
tgcttcgagcccagcaagcgttgtattaagccggatactgtggaggagtgtcaaaaagtg
gctcaaactcggtatgaagcagctctggaaattgccggcggcgaacgtcttgtttccgga
tccgacgattttacactatttatgtggaacccgaaagagacgaaacaatcgattaatcga
atgaccggacacatgcaattggtgaatcaagtcgttttctcaccggatacacgttatatc
gcctccgcctcattcgacaaatcagtgaagctctggtgtggaagaactggaaaatatttg
gcttctttgagaggacacgttggcccagtctatcaagttgcatggtctgccgacagtcgt
cttctcgtctccggatccgccgattccacgttaaaagtgttcgaattgaagacgaaaagt
ctgtactacgaccttccaggccacggagacgaagtttttactgttgattggagccccgag
gga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACACGCTCACTGGATTAACACTT 96 527 838 Antisense
GCGTTGAGAACCTCGTGCTTCGAGC 120 291 883 Antisense
TTCGAGCCCAGCAAGCGTTGTATTA 435 695 901 Antisense
AGCAGCTCTGGAAATTGCCGGCGGC 278 77 975 Antisense
GTCTTGTTTCCGGATCCGACGATTT 91 467 1004 Antisense
TGAATCAAGTCGTTTTCTCACCGGA 195 575 1100 Antisense
ACCGGATACACGTTATATCGCCTCC 667 87 1119 Antisense
ACACGTTGGCCCAGTCTATCAAGTT 492 109 1212 Antisense
TCAAGTTGCATGGTCTGCCGACAGT 486 623 1230 Antisense
AAAGTCTGTACTACGACCTTCCAGG 704 119 1313 Antisense
TACTGTTGATTGGAGCCCCGAGGGA 314 645 1356 Antisense
Sequence Source Affymetrix Proprietary Database