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[182201_at]

Description F53B3.3 /REP_DB=WormBase Gene ID /WP=CE04651 /TR=Q20712 /GB=AAA81407.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 23      4grp_high_8_8 > 45      6grp_highest_nonorm > 90      6grp_total > 12      4grp_total > 59      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 152.6
(1.1)
P 		186.8
(3)
P 		184.4
(2.9)
P 		77.4
(-3)
A 		109
B 143.7
(0.4)
P 		195.6
(3)
P 		168.6
(1.6)
P 		76.7
(-3)
A 		119
C 138.6
(-3)
P 		176.4
(3)
P 		164.4
(1.1)
P 		141.3
(-2.6)
P 		38
avr 145
(0.2) 		186.3
(3) 		172.5
(2.1) 		98.5
(-3) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 120
1 2 3 4 5 6 range
A 140.7
(1.5)
P 		167.6
(3)
P 		132.6
(1.1)
P 		113
(0.1)
P 		131.6
(1.1)
P 		56.3
(-3)
P 		111
B 114.5
(-0.7)
P 		176.3
(3)
P 		108.1
(-1.1)
P 		127.4
(0.1)
P 		75.6
(-3)
P 		76.6
(-2.9)
P 		101
C 151.3
(2.3)
P 		161.4
(3)
P 		117.5
(0.1)
P 		89.3
(-1.8)
P 		74.2
(-2.8)
P 		71.3
(-3)
P 		90
avr 135.5
(1) 		168.4
(3) 		119.4
(0.1) 		109.9
(-0.5) 		93.8
(-1.5) 		68.1
(-3) 		100
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 55.6
(3)
P 		40.5
(1)
P 		48.2
(2)
P 		11.3
(-3)
A 		44
B 45.8
(2.5)
P 		48.3
(3)
P 		39.1
(1.3)
P 		16.4
(-3)
A 		32
C 45.9
(1)
P 		50.7
(1.8)
P 		57.3
(3)
P 		23.8
(-3)
P 		34
avr 49.1
(3) 		46.5
(2.5) 		48.2
(2.8) 		17.2
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 25.9
(-1.4)
P 		59.6
(3)
P 		28.9
(-1)
P 		28.6
(-1.1)
P 		39.7
(0.4)
P 		13.7
(-3)
P 		46
B 39.9
(-0.5)
P 		58.7
(3)
P 		26.2
(-3)
P 		33.7
(-1.6)
P 		30.7
(-2.2)
P 		28.6
(-2.6)
P 		33
C 56.1
(3)
P 		49.3
(1.9)
P 		29.9
(-1.1)
P 		18
(-3)
P 		24
(-2.1)
P 		23.9
(-2.1)
P 		38
avr 40.6
(0.3) 		55.9
(3) 		28.3
(-1.9) 		26.8
(-2.2) 		31.5
(-1.3) 		22.1
(-3) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182201_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18121
Sequence Type Exemplar sequence
Representative Public ID F53B3.3 NCBI
Target Description F53B3.3 /REP_DB=WormBase Gene ID /WP=CE04651 /TR=Q20712 /GB=AAA81407.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076096(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076096 NCBI Caenorhabditis elegans F53B3.3 (F53B3.3) mRNA, complete cds. 9/11 None
GENEFINDER00000011078 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2866065:2873504:-1 9/11 None
F53B3.3 ENSEMBL cdna:known chromosome:CEL140:X:2866065:2871053:-1 gene:F53B3.3 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000011072 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2866064-2871053(-) 94.77 100.0
Public Domain and Genome References
Gene Title
Ensembl F53B3.3
Entrez Gene 180577 Entrez gene
SwissProt Q20712 EMBL-EBI
WormBase CE37117 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81407 Hypothetical protein F53B3.3 [Caenorhabditis elegans] ref|NP_508497.2| F53B3.3 [Caenorhabditis elegans] 0.0
blast CAE58821 Hypothetical protein CBG02031 [Caenorhabditis briggsae] 0.0
blast AAA81407 Hypothetical protein F53B3.3 [Caenorhabditis elegans] ref|NP_508497.2| F53B3.3 [Caenorhabditis elegans] 1.0E-155
blast CAE58821 Hypothetical protein CBG02031 [Caenorhabditis briggsae] 1.0E-105
Sequence
Target Sequence 
>C. ELEGANS:182201_AT
gacgactatctagtgcactttctccaagagcacgatttgcggatcctccgattgttgagg
ctcagcatcctcacgcaggattttcatatttgttcccacaacagccagttgtcagttcag
tgtacaacaaaaaaatgatgtcgccggaaccaagagaagtgactattttaggaaaattgg
ggtgtatgttttcgaaatttttttttggaaattttttgagtagtctcattatgaaattcg
gtcgttttggtccgcgcccctgtacgccacagcaggaaatcacgaaggctccagccggcc
cctctctcctctggaaattcagaactcaatgtccggaactcatgatttcgatatgccagt
tgcatacagtaatgagaatggacaagtggtcacacgtggggtatagaattttaagttccc
agaactgccttgaatgtgtaattttcacatttaggttcgactatactccaaatgaatctc
gcagcaatagtcgtgcaaaacttcggagcccaagcattaacggagaagatgttcgtaatg
ctctgagccagttcgattacttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGACTATCTAGTGCACTTTCTCC 46 381 1268 Antisense
GCGGATCCTCCGATTGTTGAGGCTC 442 293 1306 Antisense
CTCAGCATCCTCACGCAGGATTTTC 128 229 1328 Antisense
ATATTTGTTCCCACAACAGCCAGTT 36 17 1353 Antisense
ACAGCCAGTTGTCAGTTCAGTGTAC 444 107 1368 Antisense
TGAAATTCGGTCGTTTTGGTCCGCG 112 545 1499 Antisense
GAACTCAATGTCCGGAACTCATGAT 340 349 1589 Antisense
AGTTCCCAGAACTGCCTTGAATGTG 676 61 1681 Antisense
ATACTCCAAATGAATCTCGCAGCAA 497 23 1730 Antisense
TAGTCGTGCAAAACTTCGGAGCCCA 454 651 1755 Antisense
GCTCTGAGCCAGTTCGATTACTTGT 591 299 1807 Antisense
Sequence Source Affymetrix Proprietary Database