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[182181_at]

Description ZC308.2 /REP_DB=WormBase Gene ID /WP=CE15161 /TR=O17087 /GB=AAB70342.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 27      6grp_total > 40      4grp_total > 29      6grp_cgc > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 106.3
(3)
P 		37.8
(-3)
A 		56.4
(-1.4)
P 		98.9
(2.4)
P 		69
B 97.7
(3)
P 		95.1
(2.7)
P 		52.8
(-3)
P 		57.3
(-2.4)
M 		45
C 68.9
(-0)
P 		89.5
(3)
P 		48.8
(-3)
A 		89.5
(3)
P 		41
avr 91
(3) 		74.1
(0.4) 		52.7
(-3) 		81.9
(1.6) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 69.9
(0.7)
A 		85.8
(3)
P 		47
(-2.7)
P 		57.5
(-1.1)
A 		51
(-2.1)
A 		44.8
(-3)
A 		41
B 66.6
(2)
P 		55.9
(0.1)
A 		72.1
(3)
P 		51.1
(-0.8)
A 		38.6
(-3)
A 		52
(-0.6)
P 		34
C 72.5
(-0.5)
P 		73.5
(-0.4)
A 		95.6
(1.9)
P 		106
(3)
M 		69
(-0.8)
P 		48.3
(-3)
P 		58
avr 69.7
(2.5) 		71.7
(3) 		71.6
(3) 		71.5
(2.9) 		52.9
(-1.8) 		48.4
(-3) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 38.7
(3)
P 		8.2
(-3)
A 		14.8
(-1.7)
P 		14.5
(-1.8)
P 		31
B 31.2
(3)
P 		23.5
(0.6)
P 		12.2
(-3)
P 		12.2
(-3)
M 		19
C 22.8
(1.4)
P 		25.7
(3)
P 		17
(-1.9)
A 		15.1
(-3)
P 		11
avr 30.9
(3) 		19.1
(-1.2) 		14.7
(-2.7) 		13.9
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 12.9
(-2.2)
A 		30.5
(3)
P 		10.2
(-3)
P 		14.6
(-1.7)
A 		15.4
(-1.5)
A 		10.9
(-2.8)
A 		20
B 23.2
(3)
P 		18.6
(0.2)
A 		17.5
(-0.5)
P 		13.5
(-3)
A 		15.7
(-1.6)
A 		19.4
(0.6)
P 		10
C 26.9
(3)
P 		22.4
(0.5)
A 		24.3
(1.5)
P 		21.3
(-0.1)
M 		22.3
(0.4)
P 		16.2
(-3)
P 		11
avr 21
(1) 		23.8
(3) 		17.3
(-1.7) 		16.5
(-2.3) 		17.8
(-1.3) 		15.5
(-3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182181_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.737
Sequence Type Exemplar sequence
Representative Public ID ZC308.2 NCBI
Target Description ZC308.2 /REP_DB=WormBase Gene ID /WP=CE15161 /TR=O17087 /GB=AAB70342.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059440(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059440 NCBI Caenorhabditis elegans defective in Germ Line Development family member (gld-2) (gld-2) mRNA, complete cds. 11/11 A 
SNAP00000042587 ENSEMBL cdna:SNAP chromosome:CEL140:I:6384832:6386844:1 11/11 None
GENEFINDER00000042592 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6384832:6387312:1 11/11 None
ZC308.1c ENSEMBL cdna:known chromosome:CEL140:I:6384832:6393219:1 gene:ZC308.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_059441 7/11 Cross Hyb Matching Probes A 
NM_001026674 8/11 Cross Hyb Matching Probes None
ZC308.1a 7/11 Cross Hyb Matching Probes A 
ZC308.1d 8/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172338 4 1 NM_059440 182181_at (11)
NM_059441 172979_at (11), 174893_at (11), 182981_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6384760-6386773(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZC308.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO21478 Defective in germ line development protein 2, isoform c [Caenorhabditis elegans] ref|NP_491841.2| defective in Germ Line Development family member (gld-2) [Caenorhabditis elegans] 0.0
blast AAO21479 Defective in germ line development protein 2, isoform d [Caenorhabditis elegans] ref|NP_001021845.1| defective in Germ Line Development family member (gld-2) [Caenorhabditis elegans] 0.0
blast AAB70342 Defective in germ line development protein 2, isoform a [Caenorhabditis elegans] ref|NP_491842.2| defective in Germ Line Development family member (gld-2) [Caenorhabditis elegans] gb|AAM94369.1| regulatory cytoplasmic polyA polymerase [Caenorhabditis elegans] 0.0
blast AAO21479 Defective in germ line development protein 2, isoform d [Caenorhabditis elegans] ref|NP_001021845.1| defective in Germ Line Development family member (gld-2) [Caenorhabditis elegans] 4.0E-98
Protein Domains
Database ID Description E-Value
Pfam IPR002058 EMBL-EBI PAP/25A-associated domain 9.8E-11
Sequence
Target Sequence 
>C. ELEGANS:182181_AT
tggctccaatttatacatcttctcgaaacaccgatggtggcttggataaaaaactcgtga
aacctgccaatttcccaatgtgctgggctgtacctcaaccggcaattttctatcagttga
tggccattgatgtggtggagtatatcaagtttttagaacgaatttctccacaactgaaga
cacaacaacagtctcctggaattccgattcaaaatgaagcagaagcgtcggcagtaatga
aagcaatgagatcatttcagtttcataattggccgcagatgtcacatggatcctattatc
caatgccatatcatctggaaaatcaaatgagacctatgaaaagtggagatcagcttccgt
taaatcagcaaaaccacaatttgtcagggtttccagcgttcgtcggaaagagttctcttg
ttggatcatccttgaatacccgcaacagcagcgaagct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGCTCCAATTTATACATCTTCTCG 450 545 623 Antisense
ACTCGTGAAACCTGCCAATTTCCCA 58 99 675 Antisense
TTCCCAATGTGCTGGGCTGTACCTC 566 695 694 Antisense
TACCTCAACCGGCAATTTTCTATCA 565 649 713 Antisense
AACAGTCTCCTGGAATTCCGATTCA 186 137 809 Antisense
TTCAGTTTCATAATTGGCCGCAGAT 195 687 878 Antisense
GGCCGCAGATGTCACATGGATCCTA 274 543 893 Antisense
GTGGAGATCAGCTTCCGTTAAATCA 639 489 965 Antisense
AATTTGTCAGGGTTTCCAGCGTTCG 394 173 1000 Antisense
AGAGTTCTCTTGTTGGATCATCCTT 414 69 1031 Antisense
GAATACCCGCAACAGCAGCGAAGCT 290 327 1056 Antisense
Sequence Source Affymetrix Proprietary Database