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[182180_at]

Description C50D2.6 /REP_DB=WormBase Gene ID /WP=CE08891 /TR=O44792 /GB=AAB94948.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_highest_8_8 > 62      4grp_high_8_8 > 18      6grp_highest_nonorm > 60      6grp_total > 14      4grp_total > 30      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 315
1 2 3 4 range
A 585.2
(3)
P 		545.9
(1.7)
P 		407.6
(-3)
P 		504.2
(0.3)
P 		178
B 585
(3)
P 		436.8
(-2.2)
P 		415.3
(-3)
P 		504.7
(0.2)
P 		170
C 652
(3)
P 		470.6
(-0.5)
P 		336.9
(-3)
P 		609.9
(2.2)
P 		315
avr 607.4
(3) 		484.4
(-0.3) 		386.6
(-3) 		539.6
(1.2) 		221

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 377
1 2 3 4 5 6 range
A 539.8
(3)
P 		460.1
(1.7)
P 		336.6
(-0.3)
P 		237.6
(-2)
P 		246.4
(-1.8)
P 		173.5
(-3)
P 		366
B 535.8
(3)
P 		382
(0.2)
P 		292
(-1.4)
P 		348.1
(-0.4)
P 		270.5
(-1.8)
P 		201.8
(-3)
P 		334
C 542.8
(3)
P 		453.4
(1.6)
P 		364.6
(0.2)
P 		317.8
(-0.6)
P 		257.6
(-1.5)
P 		165.9
(-3)
P 		377
avr 539.5
(3) 		431.8
(1.2) 		331.1
(-0.5) 		301.2
(-1) 		258.2
(-1.7) 		180.4
(-3) 		359
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 213.1
(3)
P 		118.5
(-1.1)
P 		106.6
(-1.6)
P 		73.8
(-3)
P 		139
B 186.5
(3)
P 		107.9
(-2.2)
P 		96.2
(-3)
P 		107.6
(-2.2)
P 		90
C 215.8
(3)
P 		135.3
(-1.3)
P 		117.4
(-2.2)
P 		102.8
(-3)
P 		113
avr 205.1
(3) 		120.6
(-1.6) 		106.7
(-2.3) 		94.7
(-3) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 99.5
(-0.2)
P 		163.6
(3)
P 		73.3
(-1.5)
P 		60.3
(-2.1)
P 		74.4
(-1.4)
P 		42.3
(-3)
P 		121
B 186.4
(3)
P 		127.2
(-0.1)
P 		70.9
(-3)
P 		92.2
(-1.9)
P 		109.9
(-1)
P 		75.3
(-2.8)
P 		116
C 201.2
(3)
P 		138.4
(0.4)
P 		92.8
(-1.5)
P 		63.9
(-2.7)
P 		83.3
(-1.9)
P 		55.5
(-3)
P 		146
avr 162.4
(3) 		143.1
(1.9) 		79
(-1.8) 		72.1
(-2.2) 		89.2
(-1.2) 		57.7
(-3) 		105
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182180_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4087
Sequence Type Exemplar sequence
Representative Public ID C50D2.6 NCBI
Target Description C50D2.6 /REP_DB=WormBase Gene ID /WP=CE08891 /TR=O44792 /GB=AAB94948.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061255(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061255 NCBI Caenorhabditis elegans C50D2.6 (C50D2.6) mRNA, complete cds. 10/11 None
GENEFINDER00000026487 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:84406:87758:1 10/11 None
C50D2.6 ENSEMBL cdna:known chromosome:CEL140:II:84434:87758:1 gene:C50D2.6 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026467 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:84405-87758(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C50D2.6
Entrez Gene 3565416 Entrez gene
SwissProt Q8ITY7 EMBL-EBI
WormBase CE34768 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_493656 C50D2.6 [Caenorhabditis elegans] gb|AAN73871.2| Hypothetical protein C50D2.6 [Caenorhabditis elegans] 1.0E-134
blast CAE62814 Hypothetical protein CBG06990 [Caenorhabditis briggsae] 1.0E-116
Sequence
Target Sequence 
>C. ELEGANS:182180_AT
atatttgccaagcctacacacgtgtcgatttgtgtcttcactcatgcgaaaaggtgtcgc
aacactcggccagcattcggaaaacctatgcgggcatacgattcatctgtgtggagcatc
gagacgcatttctattttctagacacgaaaagattgccctccaacagtgccgacctcaga
tcaatgattctctagccgcaagcaatcgattttcggtcacagtactacggaaggagcatc
acaacctgagatctcatttcgagatgctctgcaaaaaacttggctccatgattgaatgtg
tggagcccgtaacacgagcaggctgtggagatcaagcggcaaagatgatgcttcgattca
tcactgtcggattttcgagtttcgagcagctctattctcaactgggtatctccgatcaat
tgcccgcggcttgcaagtcgctgctctcacttcaaaaacgcacggtgctccccgagcgtc
gccattatgtcctcccgcaggcttcttcggagtacagtgtcttcagttccacgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATTTGCCAAGCCTACACACGTGT 682 19 332 Antisense
GTCGATTTGTGTCTTCACTCATGCG 564 473 355 Antisense
CACTCGGCCAGCATTCGGAAAACCT 392 197 394 Antisense
GCGGGCATACGATTCATCTGTGTGG 243 293 421 Antisense
AGACACGAAAAGATTGCCCTCCAAC 670 71 472 Antisense
ACAGTGCCGACCTCAGATCAATGAT 146 107 495 Antisense
GATCAATGATTCTCTAGCCGCAAGC 540 421 510 Antisense
GGAGCATCACAACCTGAGATCTCAT 26 521 564 Antisense
ATGCTTCGATTCATCACTGTCGGAT 348 41 679 Antisense
AGCTCTATTCTCAACTGGGTATCTC 707 79 719 Antisense
AGTACAGTGTCTTCAGTTCCACGTC 478 57 842 Antisense
Sequence Source Affymetrix Proprietary Database