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[182157_at]

Description F28H1.5 /REP_DB=WormBase Gene ID /WP=CE09770 /TR=O01540 /GB=AAB52341.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 39      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 1.1
(-2.6)
A 		3.6
(0.2)
A 		0.7
(-3)
A 		6.2
(3)
A 		6
B 22.7
(3)
A 		2
(-2.8)
A 		2.3
(-2.7)
A 		1.3
(-3)
A 		21
C 2.4
(3)
A 		1.4
(-0.3)
A 		0.6
(-3)
A 		1.9
(1.3)
A 		2
avr 8.7
(3) 		2.3
(-2.1) 		1.2
(-3) 		3.1
(-1.5) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 0.8
(-3)
A 		1.4
(-2.9)
A 		1.9
(-2.8)
A 		3.5
(-2.4)
A 		28.5
(3)
A 		4.5
(-2.2)
A 		28
B 0.6
(-3)
A 		1.3
(-2.3)
A 		5.5
(2)
A 		2.3
(-1.3)
A 		2.8
(-0.8)
A 		6.5
(3)
A 		6
C 0.7
(-3)
A 		3.7
(-1.8)
A 		2.3
(-2.3)
A 		5.1
(-1.2)
A 		15.1
(3)
A 		4.4
(-1.5)
A 		14
avr 0.7
(-3) 		2.1
(-2.4) 		3.2
(-2) 		3.6
(-1.8) 		15.5
(3) 		5.1
(-1.2) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 0.4
(-1.3)
A 		0.8
(2.1)
A 		0.2
(-3)
A 		0.9
(3)
A 		1
B 7.3
(3)
A 		0.5
(-2.8)
A 		0.5
(-2.8)
A 		0.3
(-3)
A 		7
C 0.8
(3)
A 		0.4
(-1)
A 		0.2
(-3)
A 		0.3
(-2)
A 		1
avr 2.8
(3) 		0.6
(-2.3) 		0.3
(-3) 		0.5
(-2.5) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 0.1
(-3)
A 		0.5
(-2.7)
A 		0.4
(-2.8)
A 		0.9
(-2.4)
A 		8.6
(3)
A 		1.1
(-2.3)
A 		9
B 0.2
(-3)
A 		0.4
(-2.5)
A 		1.3
(0)
A 		0.6
(-1.9)
A 		1.1
(-0.5)
A 		2.4
(3)
A 		2
C 0.3
(-3)
A 		1.1
(-2)
A 		0.6
(-2.6)
A 		1
(-2.1)
A 		4.9
(3)
A 		1.5
(-1.4)
A 		5
avr 0.2
(-3) 		0.7
(-2.4) 		0.8
(-2.2) 		0.8
(-2.2) 		4.9
(3) 		1.7
(-1.1) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182157_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.251
Sequence Type Exemplar sequence
Representative Public ID F28H1.5 NCBI
Target Description F28H1.5 /REP_DB=WormBase Gene ID /WP=CE09770 /TR=O01540 /GB=AAB52341.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058879(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058879 NCBI Caenorhabditis elegans F28H1.5 (F28H1.5) mRNA, complete cds. 11/11 None
GENEFINDER00000015493 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3974129:3979511:-1 10/11 None
SNAP00000015485 ENSEMBL cdna:SNAP chromosome:CEL140:I:3978834:3979511:-1 10/11 None
F28H1.5 ENSEMBL cdna:known chromosome:CEL140:I:3978495:3979526:-1 gene:F28H1.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3978494-3979526(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28H1.5
Entrez Gene 185085 Entrez gene
SwissProt O01540 EMBL-EBI
WormBase CE09770 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001021426 F28H1.4a [Caenorhabditis elegans] gb|AAB52340.2| Hypothetical protein F28H1.4a [Caenorhabditis elegans] 1.0E-110
blast CAE66737 Hypothetical protein CBG12087 [Caenorhabditis briggsae] 2.0E-94
blast NP_001021427 F28H1.4b [Caenorhabditis elegans] gb|AAV58868.1| Hypothetical protein F28H1.4b [Caenorhabditis elegans] 2.0E-87
blast NP_491280 F28H1.5 [Caenorhabditis elegans] gb|AAB52341.1| Hypothetical protein F28H1.5 [Caenorhabditis elegans] 2.0E-63
blast NP_491280 F28H1.5 [Caenorhabditis elegans] gb|AAB52341.1| Hypothetical protein F28H1.5 [Caenorhabditis elegans] 6.0E-50
blast CAE66736 Hypothetical protein CBG12086 [Caenorhabditis briggsae] 4.0E-28
blast CAE66736 Hypothetical protein CBG12086 [Caenorhabditis briggsae] 8.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR008253 EMBL-EBI Marvel 1.9E-33
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000015493 4 242-264,269-291,304-326,331-350
Sequence
Target Sequence 
>C. ELEGANS:182157_AT
ggatttttctggaatgtcgcaaacattgggctctattagaaacaataataacgaaggaaa
caatagtggcgacttgcaatcttgcattgatgtcgggcagattatggagaatattcaaag
agaacaatttattcgccaacaaaagtctgctgaacaaaccgaaggtattacaacaagtct
gttgaattggaaaaatacaaatgattccgagatgaaaacacttgaaaatcaaatggagct
gttgagaaagagaaaagagcaaatcaagcagaactcgacggaaatgtctgcgttggcgaa
tcagttgagacagagattggagttgattcggggctccaaggagca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATTTTTCTGGAATGTCGCAAACA 72 509 15 Antisense
AATAGTGGCGACTTGCAATCTTGCA 662 171 76 Antisense
GCAATCTTGCATTGATGTCGGGCAG 527 321 90 Antisense
GATGTCGGGCAGATTATGGAGAATA 146 409 103 Antisense
AAGAGAACAATTTATTCGCCAACAA 150 153 132 Antisense
TCGCCAACAAAAGTCTGCTGAACAA 252 599 147 Antisense
ACCGAAGGTATTACAACAAGTCTGT 304 87 172 Antisense
AGCAAATCAAGCAGAACTCGACGGA 202 77 272 Antisense
ACTCGACGGAAATGTCTGCGTTGGC 409 97 287 Antisense
GTCTGCGTTGGCGAATCAGTTGAGA 384 471 300 Antisense
AGTTGATTCGGGGCTCCAAGGAGCA 272 57 335 Antisense
Sequence Source Affymetrix Proprietary Database