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[182155_at]

Description F26F4.3 /REP_DB=WormBase Gene ID /WP=CE01260 /TR=Q19821 /GB=AAA91218.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 64      6grp_high1_10_10 > 46      4grp_highest_8_8 > 28      4grp_high_8_8 > 07      6grp_highest_nonorm > 82      6grp_total > 47      4grp_total > 0      6grp_highest_sub > Cu      6grp_cgc > 26      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 172
1 2 3 4 range
A 298.2
(1.1)
P 		215.7
(-3)
P 		333.5
(2.8)
P 		337.6
(3)
P 		122
B 320.4
(1.2)
P 		268
(-3)
P 		298.7
(-0.6)
P 		343.5
(3)
P 		76
C 310.9
(-1.8)
P 		291.6
(-3)
P 		359.8
(1.3)
P 		387.5
(3)
P 		96
avr 309.8
(0.2) 		258.4
(-3) 		330.7
(1.4) 		356.2
(3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 340
1 2 3 4 5 6 range
A 315.2
(-0.8)
P 		339
(-0.1)
P 		348.6
(0.2)
P 		365
(0.7)
P 		236.1
(-3)
P 		447.2
(3)
P 		211
B 530.1
(3)
P 		312.3
(-1)
P 		322.9
(-0.8)
P 		286.7
(-1.5)
P 		207.1
(-3)
P 		365.4
(-0.1)
P 		323
C 404.7
(3)
P 		346.6
(1.4)
P 		324.3
(0.8)
P 		336.1
(1.1)
P 		189.7
(-3)
P 		366.9
(1.9)
P 		215
avr 416.7
(3) 		332.6
(0.5) 		331.9
(0.5) 		329.3
(0.5) 		211
(-3) 		393.2
(2.3) 		206
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 108.6
(3)
P 		46.8
(-3)
P 		87.2
(0.9)
P 		49.4
(-2.7)
P 		62
B 102.2
(3)
P 		66.2
(-3)
P 		69.2
(-2.5)
P 		73.2
(-1.8)
P 		36
C 102.9
(0.8)
P 		83.8
(-1.2)
P 		125.4
(3)
P 		65.3
(-3)
P 		60
avr 104.6
(3) 		65.6
(-2.6) 		93.9
(1.5) 		62.6
(-3) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 58.1
(-3)
P 		120.6
(3)
P 		75.9
(-1.3)
P 		92.6
(0.3)
P 		71.2
(-1.7)
P 		108.9
(1.9)
P 		63
B 184.4
(3)
P 		104
(-1.4)
P 		78.4
(-2.9)
P 		75.9
(-3)
P 		84.1
(-2.5)
P 		136.3
(0.3)
P 		109
C 150
(3)
P 		105.8
(0)
P 		82.5
(-1.6)
P 		67.6
(-2.6)
P 		61.4
(-3)
P 		122.8
(1.2)
P 		89
avr 130.8
(3) 		110.1
(0.9) 		78.9
(-2.3) 		78.7
(-2.3) 		72.2
(-3) 		122.7
(2.2) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182155_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6793
Sequence Type Exemplar sequence
Representative Public ID F26F4.3 NCBI
Target Description F26F4.3 /REP_DB=WormBase Gene ID /WP=CE01260 /TR=Q19821 /GB=AAA91218.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065628(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065628 NCBI Caenorhabditis elegans RhOMboid (Drosophila) related family member (rom-1) (rom-1) mRNA, complete cds. 11/11 None
SNAP00000005753 ENSEMBL cdna:SNAP chromosome:CEL140:III:4907187:4908783:1 11/11 None
GENEFINDER00000005765 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4907187:4908783:1 11/11 None
F26F4.3 ENSEMBL cdna:known chromosome:CEL140:III:4907187:4908783:1 gene:F26F4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4906071-4908783(+) 96.43 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rom-1
Ensembl F26F4.3
Entrez Gene 184989 Entrez gene
SwissProt Q19821 EMBL-EBI
WormBase CE32392 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.28179.1.S1_AT similar to rhomboid, veinlet-like 4 cfa
CANINE_2:CFAAFFX.28179.1.S1_S_AT similar to rhomboid, veinlet-like 4 cfa
CANINE_2:CFAAFFX.28180.1.S1_AT similar to rhomboid, veinlet-like 4 cfa
DROSGENOME1:147981_AT stem cell tumor dm
DROSOPHILA_2:1641652_A_AT stem cell tumor dm
CHICKEN:GGAAFFX.2126.1.S1_AT similar to rhomboid, veinlet-like 4; ventrhoid transmembrane protein gga
HG-U133_PLUS_2:233409_AT rhomboid, veinlet-like 4 (Drosophila) hs
HG-U133B:233409_AT rhomboid, veinlet-like 4 (Drosophila) hs
HU35KSUBC:RC_AA235710_AT rhomboid, veinlet-like 4 (Drosophila) hs
U133_X3P:HS2.353064.1.S1_3P_AT rhomboid, veinlet-like 4 (Drosophila) hs
U133_X3P:HS.9800.1.A1_3P_AT rhomboid, veinlet-like 4 (Drosophila) hs
U133_X3P:HS.272312.0.S1_3P_AT rhomboid, veinlet-like 4 (Drosophila) hs
HG-U133_PLUS_2:1553268_AT rhomboid, veinlet-like 4 (Drosophila) hs
HG-U133_PLUS_2:230755_AT rhomboid, veinlet-like 4 (Drosophila) hs
HG-U133B:230755_AT rhomboid, veinlet-like 4 (Drosophila) hs
HG-U133_PLUS_2:233232_AT Rhomboid, veinlet-like 4 (Drosophila) hs
HG-U133B:233232_AT Rhomboid, veinlet-like 4 (Drosophila) hs
HU35KSUBB:RC_T82254_AT Rhomboid, veinlet-like 4 (Drosophila) hs
MOE430A:1435941_AT rhomboid, veinlet-like 4 (Drosophila) mm
MOUSE430_2:1435941_AT rhomboid, veinlet-like 4 (Drosophila) mm
MOUSE430A_2:1435941_AT rhomboid, veinlet-like 4 (Drosophila) mm
MU11KSUBA:AA471748_AT rhomboid, veinlet-like 4 (Drosophila) mm
MG-U74AV2:96490_AT rhomboid, veinlet-like 4 (Drosophila) mm
MOE430A:1427579_AT rhomboid, veinlet-like 4 (Drosophila) mm
MOUSE430A_2:1427579_AT rhomboid, veinlet-like 4 (Drosophila) mm
MOUSE430_2:1427579_AT rhomboid, veinlet-like 4 (Drosophila) mm
MG-U74CV2:135119_AT Rhomboid, veinlet-like 4 (Drosophila) (Rhbdl4), mRNA mm
RAE230B:1398060_AT rhomboid, veinlet-like 4 (Drosophila) (predicted) rn
RAT230_2:1398060_AT rhomboid, veinlet-like 4 (Drosophila) (predicted) rn
RAE230B:1391113_AT rhomboid, veinlet-like 4 (Drosophila) (predicted) rn
RAT230_2:1391113_AT rhomboid, veinlet-like 4 (Drosophila) (predicted) rn
RAE230B:1397296_AT Rhomboid, veinlet-like 4 (Drosophila) (predicted) rn
RAT230_2:1397296_AT Rhomboid, veinlet-like 4 (Drosophila) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAA91218 Rhomboid (drosophila) related protein 1 [Caenorhabditis elegans] ref|NP_498029.2| RhOMboid (Drosophila) related family member (rom-1) [Caenorhabditis elegans] sp|Q19821|ROM1_CAEEL Rhomboid related protein 0.0
blast CAE71294 Hypothetical protein CBG18183 [Caenorhabditis briggsae] 1.0E-178
blast NP_957498 rhomboid, veinlet-like 2 [Danio rerio] gb|AAH48048.1| Rhomboid, veinlet-like 2 [Danio rerio] 1.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR002610 EMBL-EBI Peptidase S54, rhomboid-like protein 1.1E-45
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498029.2 7 107-129,172-194,201-223,227-249,256-275,290-312,319-341
F26F4.3 7 107-129,172-194,201-223,227-249,256-275,290-312,319-341
SNAP00000005753 7 107-129,172-194,201-223,227-249,256-275,290-312,319-341
GENEFINDER00000005765 7 107-129,172-194,201-223,227-249,256-275,290-312,319-341
Sequence
Target Sequence 
>C. ELEGANS:182155_AT
ctccgggaatcttcatttttgcgccaaaattgagaggcgaagcgtggagattcacatcct
atatgttcttgcatgctggactgaaccatcttctcggaaacgtcatcatccaactattag
tcgggattccattggaagttgctcataaaatctggaggatcggtccaatttatctgttgg
cagtgacttctggttctcttctacagtatgctattgacccgaattcgcttctagttggtg
cttccgcaggagtttatgctctcattttcgcacatgtagcaaatgttattctgaattggc
atgaaatgccactacgatggatccgagtcctggtgctcttcgtgttcatctttctggatt
tcggtggagccattcatcgtcgattctatacaaatgactgtgattctgtgtcacatcttg
cccatattgctggagcagtcactggattattctttggatatgtagtactttataatgtag
tagaacatcgaattgagaaaataattcgatatgtttgcctatttctatactctgcatttt
ttgcaaccactattatttttgtgattgttcgtcagccatattcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCCGGGAATCTTCATTTTTGCGCC 653 233 656 Antisense
GAACCATCTTCTCGGAAACGTCATC 500 345 738 Antisense
TTGGCAGTGACTTCTGGTTCTCTTC 255 707 832 Antisense
GAATTCGCTTCTAGTTGGTGCTTCC 15 325 876 Antisense
GGTGCTTCCGCAGGAGTTTATGCTC 525 501 892 Antisense
GTTTATGCTCTCATTTTCGCACATG 613 449 907 Antisense
ATGCCACTACGATGGATCCGAGTCC 372 39 961 Antisense
TTCGGTGGAGCCATTCATCGTCGAT 41 693 1015 Antisense
GTCACATCTTGCCCATATTGCTGGA 384 465 1065 Antisense
ATGTTTGCCTATTTCTATACTCTGC 684 47 1166 Antisense
GTGATTGTTCGTCAGCCATATTCCA 236 483 1216 Antisense
Sequence Source Affymetrix Proprietary Database