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[182154_at]

Description T12F5.1 /REP_DB=WormBase Gene ID /WP=CE13595 /TR=O44761 /GB=AAB96743.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 87      4grp_highest_8_8 > 14      4grp_high_8_8 > 22      6grp_highest_nonorm > 92      6grp_total > 87      4grp_total > 17      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 77.3
(-3)
P 		105.8
(-2)
P 		86
(-2.7)
P 		253.3
(3)
P 		176
B 103.5
(-3)
P 		143.1
(-1.5)
P 		159.7
(-0.8)
P 		259.7
(3)
P 		156
C 85.9
(-3)
P 		154
(-0.6)
P 		172
(0.1)
P 		254.5
(3)
P 		169
avr 88.9
(-3) 		134.3
(-1.4) 		139.2
(-1.2) 		255.8
(3) 		167

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 555
1 2 3 4 5 6 range
A 115
(-2.9)
P 		107.5
(-3)
P 		170.1
(-2.2)
P 		193.9
(-1.9)
P 		325.9
(-0.3)
P 		595.2
(3)
P 		488
B 122.3
(-2.9)
P 		124.2
(-2.9)
P 		117.5
(-3)
P 		155.1
(-2.6)
P 		371.8
(-0)
P 		632.6
(3)
P 		515
C 77.3
(-3)
P 		169
(-2)
P 		137.4
(-2.3)
P 		174
(-1.9)
P 		289.7
(-0.7)
P 		623.7
(3)
P 		546
avr 104.9
(-3) 		133.6
(-2.7) 		141.7
(-2.6) 		174.3
(-2.2) 		329.1
(-0.4) 		617.2
(3) 		512
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 28.1
(-0.7)
P 		23
(-2.8)
P 		22.5
(-3)
P 		37.1
(3)
P 		15
B 33
(-3)
P 		35.4
(-2.4)
P 		37
(-1.9)
P 		55.3
(3)
P 		22
C 28.4
(-3)
P 		44.3
(0)
P 		60
(3)
P 		42.9
(-0.2)
P 		32
avr 29.8
(-3) 		34.2
(-1.3) 		39.8
(0.9) 		45.1
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 215
1 2 3 4 5 6 range
A 21.2
(-3)
P 		38.2
(-2.2)
P 		37
(-2.2)
P 		49.2
(-1.6)
P 		98.4
(0.7)
P 		144.9
(3)
P 		124
B 42.6
(-2.6)
P 		41.3
(-2.6)
P 		28.5
(-3)
P 		41.1
(-2.6)
P 		151.1
(0.5)
P 		235.9
(3)
P 		207
C 28.7
(-3)
P 		51.6
(-2.2)
P 		35
(-2.8)
P 		35
(-2.8)
P 		93.7
(-0.8)
P 		208.8
(3)
P 		180
avr 30.8
(-3) 		43.7
(-2.5) 		33.5
(-2.9) 		41.8
(-2.6) 		114.4
(0) 		196.5
(3) 		166
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182154_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.121
Sequence Type Exemplar sequence
Representative Public ID T12F5.1 NCBI
Target Description T12F5.1 /REP_DB=WormBase Gene ID /WP=CE13595 /TR=O44761 /GB=AAB96743.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058809(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058809 NCBI Caenorhabditis elegans T12F5.1 (T12F5.1) mRNA, complete cds. 11/11 None
SNAP00000015206 ENSEMBL cdna:SNAP chromosome:CEL140:I:3741470:3742530:1 10/11 None
GENEFINDER00000015208 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3741470:3742530:1 11/11 None
T12F5.1 ENSEMBL cdna:known chromosome:CEL140:I:3741470:3742530:1 gene:T12F5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3741469-3742530(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T12F5.1
Entrez Gene 188455 Entrez gene
SwissProt O44761 EMBL-EBI
WormBase CE13595 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB96743 Hypothetical protein T12F5.1 [Caenorhabditis elegans] ref|NP_491210.1| T12F5.1 [Caenorhabditis elegans] 1.0E-110
blast AAB96743 Hypothetical protein T12F5.1 [Caenorhabditis elegans] ref|NP_491210.1| T12F5.1 [Caenorhabditis elegans] 6.0E-97
blast CAE66657 Hypothetical protein CBG11994 [Caenorhabditis briggsae] 5.0E-64
blast CAE66657 Hypothetical protein CBG11994 [Caenorhabditis briggsae] 2.0E-58
Sequence
Target Sequence 
>C. ELEGANS:182154_AT
gaaggcgagcaattcagccctatcaaaaaacgagttaatcttaatcagctcggctcggaa
aatgataaaaatccaattccactgagatcttcacccagaaagaaaatgattgcgatcgaa
gttttgccggaaaagagcatggtgtcgcctaaaaagaagccagtgggatcagttccgttc
gaattcgacgacttttcccctgtgaagtcatactccgcgagcacatcaaaatctgccgtt
aaatctacgctaacaccgatcaaatctccaacaaaacgcgcttaccagagaccattgagg
gatctcaaaatgcttctcttaccaacgccggaaaagggtcaagaagttgaaggtccggtt
gaagaattcgatgatgagacaaacgaagatgttgccaaagcacgttcctacaatccagca
gccagaaaactcggcggttcatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGGCGAGCAATTCAGCCCTATCA 228 331 199 Antisense
AGTTAATCTTAATCAGCTCGGCTCG 658 61 231 Antisense
TCCAATTCCACTGAGATCTTCACCC 265 587 270 Antisense
GATTGCGATCGAAGTTTTGCCGGAA 69 435 306 Antisense
GAAGCCAGTGGGATCAGTTCCGTTC 488 345 354 Antisense
GACGACTTTTCCCCTGTGAAGTCAT 677 379 385 Antisense
CGCTAACACCGATCAAATCTCCAAC 273 255 446 Antisense
CAAAACGCGCTTACCAGAGACCATT 439 189 470 Antisense
TCAAAATGCTTCTCTTACCAACGCC 496 627 503 Antisense
GATGTTGCCAAAGCACGTTCCTACA 620 409 586 Antisense
CAGCCAGAAAACTCGGCGGTTCATC 304 137 617 Antisense
Sequence Source Affymetrix Proprietary Database