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[182150_at]

Description R13A1.2 /REP_DB=WormBase Gene ID /WP=CE04838 /TR=Q21977 /GB=AAA81476.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 30      6grp_total > 06      4grp_total > 14      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 571
1 2 3 4 range
A 1171.9
(3)
P 		725.4
(-3)
P 		817
(-1.8)
P 		1106.9
(2.1)
P 		447
B 887.8
(-1.9)
P 		956.1
(-1.1)
P 		799.2
(-3)
P 		1296.5
(3)
P 		497
C 1261.7
(3)
P 		853.9
(-3)
P 		964.6
(-1.4)
P 		1040.8
(-0.3)
P 		408
avr 1107.1
(2.2) 		845.1
(-3) 		860.3
(-2.7) 		1148.1
(3) 		303

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1389
1 2 3 4 5 6 range
A 1358.6
(3)
P 		1090.9
(0.9)
P 		647.1
(-2.6)
P 		718.1
(-2.1)
P 		598.7
(-3)
P 		703
(-2.2)
P 		760
B 1370.4
(3)
P 		1000.5
(0.6)
P 		622.8
(-1.9)
P 		746.5
(-1)
P 		445.9
(-3)
P 		606.1
(-2)
P 		925
C 1835.3
(3)
P 		924.5
(-1.4)
P 		927.6
(-1.3)
P 		697.1
(-2.4)
P 		845.2
(-1.7)
P 		580.8
(-3)
P 		1255
avr 1521.4
(3) 		1005.3
(-0.5) 		732.5
(-2.3) 		720.6
(-2.4) 		629.9
(-3) 		630
(-3) 		892
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 269
1 2 3 4 range
A 426.7
(3)
P 		157.5
(-3)
P 		213.6
(-1.7)
P 		162
(-2.9)
P 		269
B 283.1
(3)
P 		236.1
(0.1)
P 		185.2
(-3)
P 		276.3
(2.6)
P 		98
C 417.7
(3)
P 		245.5
(-1.3)
P 		336.2
(1)
P 		175.5
(-3)
P 		242
avr 375.8
(3) 		213
(-2.7) 		245
(-1.6) 		204.6
(-3) 		171

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 540
1 2 3 4 5 6 range
A 250.4
(-0.3)
P 		388
(3)
P 		140.9
(-3)
P 		182.1
(-2)
P 		180.7
(-2)
P 		171.1
(-2.3)
P 		247
B 476.8
(3)
P 		333
(0.4)
P 		151.2
(-3)
P 		197.7
(-2.1)
P 		181.1
(-2.4)
P 		226
(-1.6)
P 		326
C 680.3
(3)
P 		282.1
(-1.4)
P 		236.1
(-1.9)
P 		140.2
(-3)
P 		273.5
(-1.5)
P 		194.4
(-2.4)
P 		540
avr 469.2
(3) 		334.4
(0.3) 		176.1
(-2.9) 		173.3
(-3) 		211.8
(-2.2) 		197.2
(-2.5) 		296
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182150_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9943
Sequence Type Exemplar sequence
Representative Public ID R13A1.2 NCBI
Target Description R13A1.2 /REP_DB=WormBase Gene ID /WP=CE04838 /TR=Q21977 /GB=AAA81476.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068740(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068740 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-158) (tag-158) mRNA, complete cds. 11/11 None
SNAP00000003735 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7213265:7217207:1 11/11 None
GENEFINDER00000003739 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7213265:7217207:1 11/11 None
R13A1.2 ENSEMBL cdna:known chromosome:CEL140:IV:7213701:7217972:1 gene:R13A1.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7213706-7217213(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-158
Ensembl R13A1.2
Entrez Gene 177495 Entrez gene
SwissProt Q21977 EMBL-EBI
WormBase CE04838 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_501141 Temporarily Assigned Gene name family member (tag-158) [Caenorhabditis elegans] gb|AAA81476.1| Temporarily assigned gene name protein 158 [Caenorhabditis elegans] 0.0
blast CAE61836 Hypothetical protein CBG05808 [Caenorhabditis briggsae] 0.0
blast CAC80545 putative Na-K-Cl cotransporter [Meloidogyne incognita] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 1.1E-14
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 1.1E-26
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 1.1E-14
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 1.1E-26
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501141.1 10 36-58,70-92,131-153,166-188,192-214,327-349,364-386,464-486,490-512,525-547
R13A1.2 10 36-58,70-92,131-153,166-188,192-214,327-349,364-386,464-486,490-512,525-547
SNAP00000003735 10 152-171,178-200,239-261,274-296,300-322,435-457,472-494,572-594,598-620,633-655
GENEFINDER00000003739 10 152-171,178-200,239-261,274-296,300-322,435-457,472-494,572-594,598-620,633-655
Sequence
Target Sequence 
>C. ELEGANS:182150_AT
gaattagccgatccagagatctccaagaatgccttcgaacggacattattaatggaagaa
cgaacaatgatgatgagagatttacaaaaagtttctggtggaggaatgtcactctcatta
ccgccagcaaatgctccacgagcaccatcaccgcttgttacatccgaaagaagagcaaat
tcaaaggactcagacgagggaacaccgacagaaagtgaagaaacaacagaaaagaagtca
acaagcactgataatgaacaagctaatcaagaaacgaagaccaagaaggagagaatgaag
gctttggacagatcgaaggtttcgaaaatgcacactgcagtacgactcaacgagttgttg
ctacaacattccgccaattctcagttgattctacttaatctgcccaagcctccggtgcac
aaagatcagcaagcacttgatgattatgttcattatttggaagtgatgaccgacaaactc
aaccgagtgattttcgtccgtgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTAGCCGATCCAGAGATCTCCA 487 323 2488 Antisense
GAGATCTCCAAGAATGCCTTCGAAC 238 397 2503 Antisense
GGAGGAATGTCACTCTCATTACCGC 657 515 2587 Antisense
ATCACCGCTTGTTACATCCGAAAGA 44 29 2634 Antisense
GACTCAGACGAGGGAACACCGACAG 478 371 2674 Antisense
AATGCACACTGCAGTACGACTCAAC 425 165 2814 Antisense
GAGTTGTTGCTACAACATTCCGCCA 545 397 2839 Antisense
TGATTCTACTTAATCTGCCCAAGCC 15 565 2873 Antisense
AAGCCTCCGGTGCACAAAGATCAGC 490 147 2893 Antisense
GTGATGACCGACAAACTCAACCGAG 647 481 2950 Antisense
CAACCGAGTGATTTTCGTCCGTGGA 402 187 2967 Antisense
Sequence Source Affymetrix Proprietary Database