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[182148_s_at]

Description F22G12.5 /REP_DB=WormBase Gene ID /WP=CE15892 /TR=O45377 /GB=CAB07369.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 38      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_total > 37      4grp_total > 39      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 153
1 2 3 4 range
A 289.6
(3)
P 		195.3
(-2.7)
P 		190.8
(-3)
P 		228.3
(-0.7)
P 		99
B 281.6
(3)
P 		192.7
(-0.7)
P 		136.4
(-3)
P 		203
(-0.2)
P 		145
C 276.2
(3)
P 		193.6
(-2.4)
P 		185.3
(-2.9)
P 		184
(-3)
P 		92
avr 282.5
(3) 		193.9
(-1.8) 		170.8
(-3) 		205.1
(-1.2) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 169
1 2 3 4 5 6 range
A 204.9
(0.5)
P 		186.2
(-0.2)
P 		109.7
(-3)
P 		130.7
(-2.2)
P 		147.7
(-1.6)
P 		272.1
(3)
P 		162
B 274
(3)
P 		212.1
(0.8)
P 		165.4
(-0.9)
P 		105.5
(-3)
P 		139.1
(-1.8)
P 		195.8
(0.2)
P 		169
C 256.2
(3)
P 		172.7
(-1.6)
P 		179.5
(-1.2)
P 		147.9
(-3)
A 		161.4
(-2.2)
P 		147.7
(-3)
P 		109
avr 245
(3) 		190.3
(0.2) 		151.5
(-1.8) 		128
(-3) 		149.4
(-1.9) 		205.2
(1) 		117
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 105.4
(3)
P 		42.4
(-2.3)
P 		49.9
(-1.6)
P 		33.4
(-3)
P 		72
B 89.8
(3)
P 		47.6
(-1.4)
P 		31.6
(-3)
P 		43.3
(-1.8)
P 		58
C 91.4
(3)
P 		55.7
(-0.5)
P 		64.6
(0.3)
P 		31
(-3)
P 		60
avr 95.5
(3) 		48.6
(-1.7) 		48.7
(-1.7) 		35.9
(-3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 37.8
(-1)
P 		66.2
(3)
P 		23.9
(-3)
P 		33.1
(-1.7)
P 		44.6
(-0.1)
P 		66.3
(3)
P 		42
B 95.3
(3)
P 		70.6
(0.8)
P 		40.2
(-1.9)
P 		27.9
(-3)
P 		56.5
(-0.5)
P 		73
(1)
P 		67
C 95
(3)
P 		52.7
(-0.9)
P 		45.7
(-1.5)
P 		29.7
(-3)
A 		52.2
(-0.9)
P 		49.5
(-1.2)
P 		65
avr 76
(3) 		63.2
(1.3) 		36.6
(-2.2) 		30.2
(-3) 		51.1
(-0.3) 		62.9
(1.3) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182148_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1884
Sequence Type Exemplar sequence
Representative Public ID F22G12.5 NCBI
Target Description F22G12.5 /REP_DB=WormBase Gene ID /WP=CE15892 /TR=O45377 /GB=CAB07369.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060906(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060906 NCBI Caenorhabditis elegans F22G12.5 (F22G12.5) mRNA, complete cds. 11/11 None
GENEFINDER00000002464 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13177020:13190553:1 11/11 None
SNAP00000002449 ENSEMBL cdna:SNAP chromosome:CEL140:I:13185312:13190553:1 11/11 None
F22G12.5 ENSEMBL cdna:known chromosome:CEL140:I:13170619:13190778:1 gene:F22G12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13170549-13190484(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F22G12.5
Entrez Gene 173181 Entrez gene
SwissProt O45377 EMBL-EBI
WormBase CE15892 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1591399_AT similar to dedicator of cytokinesis 11 cfa
CANINE_2:CFA.9339.1.A1_AT similar to dedicator of cytokinesis 11 cfa
CANINE_2:CFAAFFX.28094.1.S1_S_AT similar to dedicator of cytokinesis 11 cfa
HU35KSUBD:RC_AA609527_AT dedicator of cytokinesis 11 hs
U133_X3P:HS.104157.0.S1_3P_AT dedicator of cytokinesis 11 hs
U133_X3P:HS.107513.0.A1_3P_AT dedicator of cytokinesis 11 hs
HG-U133_PLUS_2:226875_AT dedicator of cytokinesis 11 hs
HG-U133B:226875_AT dedicator of cytokinesis 11 hs
HG-U133_PLUS_2:238356_AT dedicator of cytokinesis 11 hs
HG-U133B:238356_AT dedicator of cytokinesis 11 hs
HG-U95B:56864_AT dedicator of cytokinesis 11 hs
HG-U95B:49918_AT dedicator of cytokinesis 11 hs
HG-U95B:51382_AT Dedicator of cytokinesis 11 hs
HG-U95D:72735_AT Dedicator of cytokinesis 11 hs
HU35KSUBC:RC_AA287369_AT Dedicator of cytokinesis 11 hs
HU35KSUBD:RC_AA210718_AT Dedicator of cytokinesis 11 hs
MOE430B:1429028_AT dedicator of cytokinesis 11 mm
MOUSE430_2:1429028_AT dedicator of cytokinesis 11 mm
MOE430B:1443467_AT dedicator of cytokinesis 11 mm
MOUSE430_2:1443467_AT dedicator of cytokinesis 11 mm
MU19KSUBA:TC21359_AT Dedicator of cytokinesis 11 (Dock11), mRNA mm
MU19KSUBB:TC32255_AT Dedicator of cytokinesis 11 (Dock11), mRNA mm
MU19KSUBB:TC32255_G_AT Dedicator of cytokinesis 11 (Dock11), mRNA mm
RAE230B:1384525_AT similar to spleen specific 238 kDa protein with PH domain rn
RAT230_2:1384525_AT similar to spleen specific 238 kDa protein with PH domain rn
RG-U34B:RC_AI058912_AT Similar to spleen specific 238 kDa protein with PH domain rn
Protein Similarities
Method ID Description E-Value
blast CAB02974 Hypothetical protein F22G12.5 [Caenorhabditis elegans] emb|CAB07369.1| Hypothetical protein F22G12.5 [Caenorhabditis elegans] ref|NP_493307.1| F22G12.5 [Caenorhabditis elegans] 0.0
blast CAE60697 Hypothetical protein CBG04359 [Caenorhabditis briggsae] 0.0
blast XP_509710 PREDICTED: similar to KIAA1058 protein [Pan troglodytes] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR010703 EMBL-EBI Dedicator of cytokinesis 1.1E-36
Pfam IPR010703 EMBL-EBI Dedicator of cytokinesis 1.1E-36
Pfam IPR001849 EMBL-EBI Pleckstrin-like 8.7E-7
Pfam IPR010703 EMBL-EBI Dedicator of cytokinesis 1.1E-36
Sequence
Target Sequence 
>C. ELEGANS:182148_S_AT
aacgaactgttctccgagtatctccatccccggtttctgaagattctcgagctgccaccg
gtttcccggcaactaggcgtcggcttcccgttatttcagtgcactttgaacagttctcac
cgctggaattcgcttgtcagaagcttaatacgaaagccgaacaaattagaaaaacgctga
atgcggcgtcaaatggacgacaattggatgtcaaggggctgcagctcttgttacagggtg
ccgtgttaccgacagtcaacgccggaccgctcgcctacgcagaagtgttcacgaaagaag
aacaacgagaaagatatggagacgatgggcttgtcaagttgagagagtcgtttagaaacc
tgatgaactcttgtcaattggcaattgaagcaaatgcatcggcaatcggaagtgatcaac
agacgtatcatgaagttcttgtttcctcatttgacgcaatgcatgaacgacttcaaacgt
tctttggagcatctctacgcggaagcctcgaacctgatggatcttcacaattcccgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGAACTGTTCTCCGAGTATCTCC 484 141 6551 Antisense
CATCCCCGGTTTCTGAAGATTCTCG 502 209 6575 Antisense
TTCCCGTTATTTCAGTGCACTTTGA 466 609 6635 Antisense
GCACTTTGAACAGTTCTCACCGCTG 157 317 6651 Antisense
TCACCGCTGGAATTCGCTTGTCAGA 593 625 6667 Antisense
ATTGGATGTCAAGGGGCTGCAGCTC 693 3 6753 Antisense
GCTGCAGCTCTTGTTACAGGGTGCC 479 295 6768 Antisense
TGTTACCGACAGTCAACGCCGGACC 555 555 6794 Antisense
GTTCTTGTTTCCTCATTTGACGCAA 26 445 6985 Antisense
CGGAAGCCTCGAACCTGATGGATCT 11 241 7050 Antisense
CTGATGGATCTTCACAATTCCCGCC 244 239 7064 Antisense
Sequence Source Affymetrix Proprietary Database