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[182147_at]

Description T20F10.2 /REP_DB=WormBase Gene ID /WP=CE21191 /TR=O18092 /GB=CAB04747.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 50      4grp_highest_8_8 > 43      4grp_high_8_8 > 48      6grp_highest_nonorm > 52      6grp_total > 51      4grp_total > 55      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 406
1 2 3 4 range
A 550
(0.3)
P 		604.1
(3)
P 		557.5
(0.7)
P 		483.3
(-3)
P 		121
B 866.6
(3)
P 		473.3
(-3)
P 		534.3
(-2.1)
P 		605.5
(-1)
P 		393
C 754
(3)
P 		460.7
(-3)
P 		632
(0.5)
P 		485.3
(-2.5)
P 		293
avr 723.5
(3) 		512.7
(-3) 		574.6
(-1.2) 		524.7
(-2.7) 		211

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 657
1 2 3 4 5 6 range
A 515.8
(-1.5)
P 		702.7
(0.8)
P 		786.3
(1.9)
P 		877.1
(3)
P 		530.6
(-1.3)
P 		397.2
(-3)
P 		480
B 760
(1.2)
P 		553.7
(-0.6)
P 		953.4
(3)
P 		872.6
(2.3)
P 		517.1
(-1)
P 		296.3
(-3)
P 		657
C 748
(1)
P 		569.7
(-0.9)
P 		938.9
(3)
P 		775.4
(1.3)
P 		518.1
(-1.4)
P 		366.9
(-3)
P 		572
avr 674.6
(0.6) 		608.7
(-0.2) 		892.9
(3) 		841.7
(2.4) 		521.9
(-1.1) 		353.5
(-3) 		539
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 206
1 2 3 4 range
A 200.2
(3)
P 		131.1
(-0.2)
P 		145.7
(0.5)
P 		70.8
(-3)
P 		129
B 276.3
(3)
P 		116.9
(-3)
P 		123.8
(-2.7)
P 		129
(-2.5)
P 		159
C 249.6
(3)
P 		132.5
(-1.2)
P 		220.3
(2)
P 		81.8
(-3)
P 		168
avr 242
(3) 		126.8
(-1.7) 		163.3
(-0.2) 		93.9
(-3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 182
1 2 3 4 5 6 range
A 95.1
(-3)
P 		249.9
(3)
P 		171.2
(-0.1)
P 		222.5
(1.9)
P 		160.1
(-0.5)
P 		96.7
(-2.9)
P 		155
B 264.4
(3)
P 		184.3
(-0.1)
P 		231.5
(1.7)
P 		231.1
(1.7)
P 		210.1
(0.9)
P 		110.5
(-3)
P 		154
C 277.2
(3)
P 		173.8
(-1)
P 		239
(1.5)
P 		155.9
(-1.7)
P 		167.7
(-1.3)
P 		122.8
(-3)
P 		154
avr 212.2
(2.9) 		202.7
(2.4) 		213.9
(3) 		203.2
(2.4) 		179.3
(1) 		110
(-3) 		104
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182147_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1468
Sequence Type Exemplar sequence
Representative Public ID T20F10.2 NCBI
Target Description T20F10.2 /REP_DB=WormBase Gene ID /WP=CE21191 /TR=O18092 /GB=CAB04747.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060299(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060299 NCBI Caenorhabditis elegans T20F10.2 (T20F10.2) mRNA, complete cds. 11/11 None
GENEFINDER00000031266 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10306431:10308498:1 11/11 None
SNAP00000031262 ENSEMBL cdna:SNAP chromosome:CEL140:I:10307428:10308498:1 11/11 None
T20F10.2 ENSEMBL cdna:known chromosome:CEL140:I:10306390:10308709:1 gene:T20F10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10306361-10308429(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20F10.2
Entrez Gene 172897 Entrez gene
SwissProt O18092 EMBL-EBI
WormBase CE21191 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04747 Hypothetical protein T20F10.2 [Caenorhabditis elegans] ref|NP_492700.1| T20F10.2 [Caenorhabditis elegans] 1.0E-105
blast CAB04747 Hypothetical protein T20F10.2 [Caenorhabditis elegans] ref|NP_492700.1| T20F10.2 [Caenorhabditis elegans] 1.0E-101
blast CAB04747 Hypothetical protein T20F10.2 [Caenorhabditis elegans] ref|NP_492700.1| T20F10.2 [Caenorhabditis elegans] 1.0E-97
blast CAE73789 Hypothetical protein CBG21339 [Caenorhabditis briggsae] 1.0E-77
blast CAE73789 Hypothetical protein CBG21339 [Caenorhabditis briggsae] 1.0E-73
blast CAE73789 Hypothetical protein CBG21339 [Caenorhabditis briggsae] 2.0E-70
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492700.1 1 21-43
T20F10.2 1 21-43
GENEFINDER00000031266 2 12-34,49-68
SNAP00000031262 1 5-22
Sequence
Target Sequence 
>C. ELEGANS:182147_AT
gaagaactatcagcgctcaaggaaaaagctgaagagcttcaacgaaacaacactgcattg
aattctcttgtctccacacaagcacaactcatcaaccaactcaatcagactattggaatt
attcaattcgaaaaggaagaactcaaaaacgctgctcaccttaacaacttgactccaaaa
atcgagaagccaaaggataagcaagcggaagagcaaaacgcagaagagcaacataaggaa
aaagaagttactgtgacgactgtaaccacacaacattcacgggaagaagctcaagtaaaa
gaggataatcaggcacaatccacaacaacagttacatcaacggagagtcctgttggaaag
ttggatgagaagaatccagagattgaagaacctgagccaattgtcgccgagccagatgca
gtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGAACTATCAGCGCTCAAGGAAA 323 339 406 Antisense
AGCTTCAACGAAACAACACTGCATT 305 79 440 Antisense
ACTGCATTGAATTCTCTTGTCTCCA 534 97 457 Antisense
TCTTGTCTCCACACAAGCACAACTC 685 617 471 Antisense
AACTCATCAACCAACTCAATCAGAC 697 139 491 Antisense
GAAGAACTCAAAAACGCTGCTCACC 166 341 541 Antisense
GCTGCTCACCTTAACAACTTGACTC 118 297 556 Antisense
GACTGTAACCACACAACATTCACGG 17 377 663 Antisense
AGGCACAATCCACAACAACAGTTAC 39 55 716 Antisense
GAAGAACCTGAGCCAATTGTCGCCG 138 341 790 Antisense
AATTGTCGCCGAGCCAGATGCAGTT 439 177 804 Antisense
Sequence Source Affymetrix Proprietary Database