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[182143_s_at]

Description F42C5.7 /REP_DB=WormBase Gene ID /WP=CE04560 /TR=Q20327 /GB=AAA81484.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 12      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 22      6grp_total > 24      4grp_total > 57      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6140
1 2 3 4 range
A 1029.2
(-2.1)
P 		5463.8
(2.3)
P 		6231.2
(3)
P 		91.2
(-3)
P 		6140
B 966.8
(-2)
P 		4724.9
(2.2)
P 		5475.6
(3)
P 		98.2
(-3)
P 		5377
C 1070.1
(-2)
P 		4133
(1.4)
P 		5591.4
(3)
P 		156.3
(-3)
P 		5435
avr 1022
(-2) 		4773.9
(1.9) 		5766.1
(3) 		115.2
(-3) 		5651

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1856
1 2 3 4 5 6 range
A 1855.3
(1.2)
P 		1742.4
(0.6)
P 		2186.7
(3)
P 		1258.5
(-2)
P 		1180.9
(-2.4)
P 		1073.8
(-3)
P 		1113
B 2347.8
(3)
P 		2093.7
(2)
P 		2037.4
(1.7)
P 		1368.1
(-1)
P 		1019.5
(-2.4)
P 		883.6
(-3)
P 		1464
C 2593.1
(3)
P 		2042.8
(1.2)
P 		1813.5
(0.5)
P 		1251.2
(-1.3)
P 		1252.6
(-1.3)
P 		736.8
(-3)
P 		1856
avr 2265.4
(3) 		1959.6
(1.7) 		2012.5
(1.9) 		1292.6
(-1.3) 		1151
(-1.9) 		898.1
(-3) 		1367
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1936
1 2 3 4 range
A 374.7
(-1.7)
P 		1186
(1.4)
P 		1629
(3)
P 		13.3
(-3)
P 		1616
B 308.3
(-1.6)
P 		1166.9
(2.5)
P 		1268.8
(3)
P 		20.9
(-3)
P 		1248
C 354.2
(-2)
P 		1188.3
(0.6)
P 		1948.8
(3)
P 		26.4
(-3)
P 		1922
avr 345.7
(-1.8) 		1180.4
(1.4) 		1615.5
(3) 		20.2
(-3) 		1595

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 715
1 2 3 4 5 6 range
A 342
(-1.7)
P 		619.7
(3)
P 		476.2
(0.6)
P 		319.2
(-2)
P 		356.3
(-1.4)
P 		261.4
(-3)
P 		358
B 816.9
(3)
P 		696.9
(1.5)
P 		494.7
(-1)
P 		362.4
(-2.6)
P 		414.2
(-2)
P 		329.5
(-3)
P 		487
C 961.2
(3)
P 		623.3
(0.2)
P 		461.6
(-1.2)
P 		251.6
(-3)
P 		405.3
(-1.7)
P 		246.7
(-3)
P 		715
avr 706.7
(3) 		646.6
(2.2) 		477.5
(-0.2) 		311.1
(-2.6) 		391.9
(-1.4) 		279.2
(-3) 		428
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182143_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8569
Sequence Type Exemplar sequence
Representative Public ID F42C5.7 NCBI
Target Description F42C5.7 /REP_DB=WormBase Gene ID /WP=CE04560 /TR=Q20327 /GB=AAA81484.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068765(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068765 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-4) (grl-4) mRNA, complete cds. 11/11 None
SNAP00000034681 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7310009:7310742:-1 11/11 None
GENEFINDER00000034688 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7308470:7311167:-1 11/11 None
F42C5.7 ENSEMBL cdna:known chromosome:CEL140:IV:7309820:7310744:-1 gene:F42C5.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7310014-7311295(-) 83.27 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-4
Ensembl F42C5.7
Entrez Gene 3564844 Entrez gene
SwissProt Q20327 EMBL-EBI
WormBase CE34016 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
7601 visual perception inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
5199 structural constituent of cell wall inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
45735 nutrient reservoir activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81483 Hypothetical protein F42C5.6 [Caenorhabditis elegans] ref|NP_501165.2| F42C5.6 [Caenorhabditis elegans] 8.0E-84
blast AAA81484 Ground-like (grd related) protein 4 [Caenorhabditis elegans] ref|NP_501166.2| GRound-Like (grd related) family member (grl-4) [Caenorhabditis elegans] 6.0E-74
blast AAA81484 Ground-like (grd related) protein 4 [Caenorhabditis elegans] ref|NP_501166.2| GRound-Like (grd related) family member (grl-4) [Caenorhabditis elegans] 7.0E-73
blast CAE61854 Hypothetical protein CBG05832 [Caenorhabditis briggsae] 2.0E-69
blast CAE61854 Hypothetical protein CBG05832 [Caenorhabditis briggsae] 2.0E-68
blast AAK39260 Ground-like (grd related) protein 6 [Caenorhabditis elegans] ref|NP_509084.1| GRound-Like (grd related) family member (grl-6) [Caenorhabditis elegans] 2.0E-22
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 5.1E-37
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 5.1E-37
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000034688 2 67-89,390-412
Sequence
Target Sequence 
>C. ELEGANS:182143_S_AT
ttctcttttcaatcatccctatagcatcttcaacattcggaggatgttgttcaatgggac
caccaccatgtcctccacctccaccaccaatgtgtgcacctccacctcttccgtgccctc
cacctccaatctgcccacctcaattctgtcctccaccaccgatgtgtcctccaccaccac
ctccaccaccaccgccgatgtgcccaccgccaccaccaccgatgccatcatattccccat
gtcaatcatatgctccagctccggtattcaatcaatatgccatgcaaccagccaacgatt
gttgctgcagatgtggaagcccatgtagattcatggctagacatagaactcatggatcaa
agctgttcaccactgaggatccagaggaagatccaacctgcaacagcaagaaactcagaa
gagttatggagaggaacatgaacggtgacccatcgatctcaaagcgtgccatccaaaagg
ctgtcgaggagaagatgttcggaaaattcaatgttatctgcgcaagaggagacttctcct
acgtagcatac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTCTTTTCAATCATCCCTATAGC 265 693 152 Antisense
TCCCTATAGCATCTTCAACATTCGG 691 595 167 Antisense
GGATGTTGTTCAATGGGACCACCAC 549 513 193 Antisense
TTCCCCATGTCAATCATATGCTCCA 613 697 384 Antisense
TCCAGCTCCGGTATTCAATCAATAT 339 589 405 Antisense
CAATATGCCATGCAACCAGCCAACG 120 181 424 Antisense
GTTGCTGCAGATGTGGAAGCCCATG 396 439 452 Antisense
AAGCTGTTCACCACTGAGGATCCAG 426 149 511 Antisense
GAAGATCCAACCTGCAACAGCAAGA 701 337 538 Antisense
ATGAACGGTGACCCATCGATCTCAA 671 43 589 Antisense
AGGAGACTTCTCCTACGTAGCATAC 60 57 678 Antisense
Sequence Source Affymetrix Proprietary Database