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[182142_at]

Description F28H1.1 /REP_DB=WormBase Gene ID /WP=CE09766 /TR=O01543 /GB=AAB52337.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 56      4grp_high_8_8 > 21      6grp_total > 57      4grp_total > 09      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 107.7
(-3)
P 		183.6
(0.5)
P 		160.7
(-0.6)
P 		238.7
(3)
P 		131
B 211.2
(2)
P 		125.8
(-3)
A 		143.4
(-2)
P 		228.7
(3)
P 		103
C 115.6
(-3)
P 		149.8
(-1.7)
M 		155.3
(-1.5)
P 		273.4
(3)
P 		158
avr 144.8
(-3) 		153.1
(-2.5) 		153.1
(-2.5) 		246.9
(3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 212
1 2 3 4 5 6 range
A 172.3
(-1.3)
P 		138.1
(-2.4)
P 		138.7
(-2.4)
P 		117.9
(-3)
P 		128.6
(-2.7)
A 		315.5
(3)
P 		198
B 195.7
(-0)
P 		192.4
(-0.2)
P 		137.9
(-3)
P 		195
(-0.1)
P 		141.9
(-2.8)
P 		255.2
(3)
P 		117
C 190.7
(-0.2)
P 		210
(0.4)
P 		134.6
(-2)
P 		161.5
(-1.2)
P 		103.6
(-3)
P 		292.7
(3)
P 		189
avr 186.2
(-0.7) 		180.2
(-1) 		137.1
(-2.5) 		158.1
(-1.8) 		124.7
(-3) 		287.8
(3) 		163
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 39.2
(0.6)
P 		39.8
(1.1)
P 		42
(3)
P 		34.9
(-3)
P 		7
B 67.3
(3)
P 		31.1
(-3)
A 		33.2
(-2.7)
P 		48.7
(-0.1)
P 		36
C 38.3
(-3)
P 		43.1
(-1.2)
M 		54.1
(3)
P 		46.1
(-0)
P 		16
avr 48.3
(3) 		38
(-3) 		43.1
(-0) 		43.2
(0) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 31.8
(-2.8)
P 		49.1
(-0.5)
P 		30.2
(-3)
P 		29.9
(-3)
P 		38.8
(-1.9)
A 		76.8
(3)
P 		47
B 68.1
(0.4)
P 		64
(-0)
P 		33.5
(-3)
P 		51.7
(-1.2)
P 		57.7
(-0.6)
P 		95.2
(3)
P 		62
C 70.7
(0.5)
P 		64.1
(-0.1)
P 		34.3
(-2.8)
P 		32.5
(-3)
P 		33.5
(-2.9)
P 		98
(3)
P 		66
avr 56.9
(-0.5) 		59.1
(-0.2) 		32.7
(-3) 		38
(-2.4) 		43.3
(-1.9) 		90
(3) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182142_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.296
Sequence Type Exemplar sequence
Representative Public ID F28H1.1 NCBI
Target Description F28H1.1 /REP_DB=WormBase Gene ID /WP=CE09766 /TR=O01543 /GB=AAB52337.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058882(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058882 NCBI Caenorhabditis elegans F28H1.1 (F28H1.1) mRNA, complete cds. 11/11 None
SNAP00000014625 ENSEMBL cdna:SNAP chromosome:CEL140:I:3995964:3996404:1 9/11 None
GENEFINDER00000014632 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3995964:3996404:1 9/11 None
F28H1.1 ENSEMBL cdna:known chromosome:CEL140:I:3995739:3996404:1 gene:F28H1.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3995738-3996404(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28H1.1
Entrez Gene 171987 Entrez gene
SwissProt O01543 EMBL-EBI
WormBase CE09766 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491283 F28H1.1 [Caenorhabditis elegans] gb|AAB52337.1| Hypothetical protein F28H1.1 [Caenorhabditis elegans] 4.0E-89
blast NP_491283 F28H1.1 [Caenorhabditis elegans] gb|AAB52337.1| Hypothetical protein F28H1.1 [Caenorhabditis elegans] 4.0E-41
blast CAE66733 Hypothetical protein CBG12082 [Caenorhabditis briggsae] 6.0E-14
blast CAE66733 Hypothetical protein CBG12082 [Caenorhabditis briggsae] 5.0E-10
Sequence
Target Sequence 
>C. ELEGANS:182142_AT
gagacagccttcaaagtccagtcaaacccatggaagaactctccagaaccatttcttcca
atgagcacccaacagccaaagtcttgggcagatgttggaaaggttgcaggacccccaaga
aactaccatcatccatatgaagtcaaaccatttgaggcgattgatgagaagaatgatgtg
ttcagtactatgggtcattggaattctgagcagaagcagagtcgacaacagcatcagcaa
taccagcagaaccagtatcaagcccgtcaacatcagtactatcagaacccacctccattc
taccaaccggagcagcctgtgcactatcaatctgctcagcaggatgtctttgataacatt
ggcgaatgggacaatagcactccaatcccatcgcaaatttctccacttcaactggatctt
accttgaccactccacttccaaccttccaaccatatccatcaactcgttcatattccttg
gagtctccaaccacatcggtcacttcaaatgaggagaagaacagctggaagacgatcgaa
ttaatcctcaaccaaaagtcgagccgttcgcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGACAGCCTTCAAAGTCCAGTCAA 353 393 49 Antisense
TTTCTTCCAATGAGCACCCAACAGC 579 671 100 Antisense
CCAGCAGAACCAGTATCAAGCCCGT 199 273 291 Antisense
CAAGCCCGTCAACATCAGTACTATC 171 185 307 Antisense
GCCTGTGCACTATCAATCTGCTCAG 292 283 363 Antisense
GGGACAATAGCACTCCAATCCCATC 143 495 416 Antisense
TCGCAAATTTCTCCACTTCAACTGG 698 599 439 Antisense
CAACTGGATCTTACCTTGACCACTC 10 189 457 Antisense
CCAACCATATCCATCAACTCGTTCA 152 277 495 Antisense
CGTTCATATTCCTTGGAGTCTCCAA 137 247 514 Antisense
CAACCAAAAGTCGAGCCGTTCGCCA 526 185 597 Antisense
Sequence Source Affymetrix Proprietary Database