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[182141_at]

Description C43H6.8 /REP_DB=WormBase Gene ID /WP=CE06959 /TR=Q18590 /GB=AAA96089.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 60      4grp_total > 12      6grp_cgc > 94      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 14.9
(-2.1)
A 		8
(-3)
A 		30.1
(-0.2)
A 		55.4
(3)
M 		47
B 30
(-1.3)
A 		50.5
(3)
A 		21.9
(-3)
A 		38.8
(0.5)
A 		29
C 45.7
(1)
A 		7.2
(-2.9)
A 		6.1
(-3)
A 		65
(3)
A 		59
avr 30.2
(-1.1) 		21.9
(-2.6) 		19.4
(-3) 		53.1
(3) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 57.9
(3)
A 		26
(-3)
A 		49.1
(1.3)
A 		45.3
(0.6)
A 		44.6
(0.5)
A 		38.8
(-0.6)
A 		32
B 20.9
(-2.5)
A 		18.2
(-3)
A 		36.4
(0.7)
A 		47.7
(3)
A 		25.2
(-1.6)
A 		34.3
(0.3)
A 		30
C 33.7
(-0.7)
A 		48.3
(0.9)
A 		33.3
(-0.7)
A 		67.3
(3)
A 		12.8
(-3)
A 		33.6
(-0.7)
A 		55
avr 37.5
(-0.7) 		30.8
(-2.2) 		39.6
(-0.2) 		53.4
(3) 		27.5
(-3) 		35.6
(-1.1) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 5.4
(0.5)
A 		1.7
(-3)
A 		7.9
(2.8)
A 		8.1
(3)
M 		6
B 9.6
(0.6)
A 		12.5
(3)
A 		5.1
(-3)
A 		8.3
(-0.4)
A 		7
C 15.1
(3)
A 		2.1
(-3)
A 		2.1
(-3)
A 		11
(1.1)
A 		13
avr 10
(3) 		5.4
(-2.5) 		5
(-3) 		9.1
(1.9) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 10.7
(-0.9)
A 		9.2
(-3)
A 		10.7
(-0.9)
A 		11.5
(0.2)
A 		13.5
(3)
A 		9.4
(-2.7)
A 		4
B 7.3
(-1.9)
A 		6.1
(-3)
A 		8.9
(-0.5)
A 		12.6
(2.8)
A 		10.3
(0.8)
A 		12.8
(3)
A 		7
C 12.5
(1.8)
A 		14.7
(3)
A 		8.5
(-0.5)
A 		13.5
(2.3)
A 		4.1
(-3)
A 		11.3
(1.1)
A 		11
avr 10.2
(-1.3) 		10
(-1.7) 		9.4
(-2.8) 		12.5
(3) 		9.3
(-3) 		11.2
(0.6) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182141_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18609
Sequence Type Exemplar sequence
Representative Public ID C43H6.8 NCBI
Target Description C43H6.8 /REP_DB=WormBase Gene ID /WP=CE06959 /TR=Q18590 /GB=AAA96089.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076039(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076039 NCBI Caenorhabditis elegans Helix Loop Helix family member (hlh-15) (hlh-15) mRNA, complete cds. 11/11 None
SNAP00000018906 ENSEMBL cdna:SNAP chromosome:CEL140:X:2412963:2413282:-1 11/11 None
GENEFINDER00000018914 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2412963:2413282:-1 11/11 None
C43H6.8 ENSEMBL cdna:known chromosome:CEL140:X:2412963:2413282:-1 gene:C43H6.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2412962-2413282(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hlh-15
Ensembl C43H6.8
Entrez Gene 183427 Entrez gene
SwissProt Q18590 EMBL-EBI
WormBase CE06959 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.19477.1.S1_AT similar to Helix-loop-helix protein 1 (HEN1) (Nescient helix loop helix 1) (NSCL-1) cfa
DROSOPHILA_2:1628371_AT Helix loop helix protein 4C dm
DROSGENOME1:143644_AT Helix loop helix protein 4C dm
CHICKEN:GGA.141.1.S2_AT similar to basic helix-loop-helix protein NSCL1 gga
HG-U133_PLUS_2:214628_AT nescient helix loop helix 1 hs
HG-U133A_2:214628_AT nescient helix loop helix 1 hs
HG-FOCUS:214628_AT nescient helix loop helix 1 hs
HG-U133A:214628_AT nescient helix loop helix 1 hs
HU35KSUBD:RC_H09936_S_AT nescient helix loop helix 1 hs
HUGENEFL:M96739_AT nescient helix loop helix 1 hs
U133_X3P:HS.30956.0.S2_3P_AT nescient helix loop helix 1 hs
HG-U95AV2:36281_AT nescient helix loop helix 1 hs
MOE430A:1419533_AT nescient helix loop helix 1 mm
MOUSE430A_2:1419533_AT nescient helix loop helix 1 mm
MOUSE430_2:1419533_AT nescient helix loop helix 1 mm
MU11KSUBA:M82874_S_AT nescient helix loop helix 1 mm
MG-U74AV2:95929_AT nescient helix loop helix 1 mm
MG-U74CV2:171195_F_AT nescient helix loop helix 1 mm
Protein Similarities
Method ID Description E-Value
blast AAA96089 Helix loop helix protein 15 [Caenorhabditis elegans] ref|NP_508440.1| Helix Loop Helix family member (hlh-15) [Caenorhabditis elegans] 7.0E-30
blast CAE58879 Hypothetical protein CBG02112 [Caenorhabditis briggsae] 5.0E-29
blast XP_424510 PREDICTED: similar to basic helix-loop-helix protein NSCL1 [Gallus gallus] 7.0E-15
Protein Domains
Database ID Description E-Value
Pfam IPR001092 EMBL-EBI Basic helix-loop-helix dimerization domain bHLH 1.8E-17
Sequence
Target Sequence 
>C. ELEGANS:182141_AT
gatggaggattggacaccacgtctgaagaataccgaaaactttcaaaagccgaacgaagg
aagaggagacgtgcaactccaaaatatcgaaatcttcatgcgacccgagaaagaattcgt
gtagaatcattcaatatggcattttcccaactgcgagctctcctgccaactcttcccgtt
gaaaagaaactttcaaaaattgaaattctacgattctcaattgctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGGAGGATTGGACACCACGTCTG 314 407 25 Antisense
GGACACCACGTCTGAAGAATACCGA 638 525 36 Antisense
GGAAGAGGAGACGTGCAACTCCAAA 546 531 83 Antisense
GACGTGCAACTCCAAAATATCGAAA 310 377 92 Antisense
ATATCGAAATCTTCATGCGACCCGA 185 21 108 Antisense
GAAATCTTCATGCGACCCGAGAAAG 325 363 113 Antisense
CATGCGACCCGAGAAAGAATTCGTG 35 209 121 Antisense
GAATTCGTGTAGAATCATTCAATAT 205 325 137 Antisense
AATATGGCATTTTCCCAACTGCGAG 221 171 157 Antisense
CTGCCAACTCTTCCCGTTGAAAAGA 573 235 187 Antisense
GAAATTCTACGATTCTCAATTGCTT 19 365 226 Antisense
Sequence Source Affymetrix Proprietary Database