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[182123_at]

Description K08A2.4 /REP_DB=WormBase Gene ID /WP=CE23861 /TR=Q9N5J4 /GB=AAF39917.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 58      4grp_total > 06      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 203
1 2 3 4 range
A 127.8
(1.2)
A 		72
(-3)
A 		126.6
(1.1)
A 		152.1
(3)
P 		80
B 98.4
(-2.2)
A 		77.8
(-3)
A 		101.8
(-2.1)
A 		241.2
(3)
P 		163
C 184.4
(3)
A 		38.6
(-3)
A 		48.4
(-2.6)
A 		169.8
(2.4)
P 		146
avr 136.9
(0.6) 		62.8
(-3) 		92.3
(-1.6) 		187.7
(3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 166
(3)
A 		92.7
(-3)
A 		97.8
(-2.6)
A 		135.2
(0.5)
A 		127.1
(-0.2)
A 		119.3
(-0.8)
A 		73
B 140.4
(1.2)
A 		93.3
(-2.1)
A 		108.1
(-1.1)
A 		81.1
(-3)
A 		115.4
(-0.6)
A 		165.7
(3)
A 		85
C 128.6
(-0)
A 		89.4
(-2.7)
A 		108.2
(-1.4)
A 		94
(-2.4)
A 		85.5
(-3)
A 		173
(3)
A 		88
avr 145
(2.2) 		91.8
(-3) 		104.7
(-1.7) 		103.4
(-1.9) 		109.3
(-1.3) 		152.7
(3) 		61
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 46.5
(3)
A 		15.6
(-3)
A 		33.1
(0.4)
A 		22.3
(-1.7)
P 		31
B 31.4
(-0.7)
A 		19.2
(-3)
A 		23.6
(-2.2)
A 		51.4
(3)
P 		32
C 61
(3)
A 		11.1
(-3)
A 		16.9
(-2.3)
A 		28.6
(-0.9)
P 		50
avr 46.3
(3) 		15.3
(-3) 		24.5
(-1.2) 		34.1
(0.6) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 30.6
(0.3)
A 		33
(1.1)
A 		21.3
(-3)
A 		34.3
(1.6)
A 		38.4
(3)
A 		29
(-0.3)
A 		17
B 48.8
(1.1)
A 		31.1
(-1.6)
A 		26.2
(-2.3)
A 		21.5
(-3)
A 		46.9
(0.8)
A 		61.8
(3)
A 		40
C 47.7
(1.4)
A 		27.3
(-1.7)
A 		27.5
(-1.7)
A 		18.9
(-3)
A 		27.7
(-1.6)
A 		57.9
(3)
A 		39
avr 42.4
(1.3) 		30.5
(-1.6) 		25
(-3) 		24.9
(-3) 		37.7
(0.1) 		49.6
(3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182123_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4205
Sequence Type Exemplar sequence
Representative Public ID K08A2.4 NCBI
Target Description K08A2.4 /REP_DB=WormBase Gene ID /WP=CE23861 /TR=Q9N5J4 /GB=AAF39917.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062016(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062016 NCBI Caenorhabditis elegans K08A2.4 (K08A2.4) mRNA, complete cds. 11/11 A 
SNAP00000036644 ENSEMBL cdna:SNAP chromosome:CEL140:II:2989066:2991195:1 11/11 None
GENEFINDER00000036651 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2988032:2991195:1 11/11 None
K08A2.4 ENSEMBL cdna:known chromosome:CEL140:II:2988301:2991233:1 gene:K08A2.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2988305-2991194(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08A2.4
Entrez Gene 173643 Entrez gene
SwissProt Q9N5J4 EMBL-EBI
WormBase CE23861 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39917 Hypothetical protein K08A2.4 [Caenorhabditis elegans] ref|NP_494417.1| K08A2.4 [Caenorhabditis elegans] 0.0
blast AAF39917 Hypothetical protein K08A2.4 [Caenorhabditis elegans] ref|NP_494417.1| K08A2.4 [Caenorhabditis elegans] 1.0E-128
blast AAB70946 Hypothetical protein B0454.9 [Caenorhabditis elegans] ref|NP_494435.1| B0454.9 [Caenorhabditis elegans] 5.0E-46
blast AAB70946 Hypothetical protein B0454.9 [Caenorhabditis elegans] ref|NP_494435.1| B0454.9 [Caenorhabditis elegans] 6.0E-46
blast AAB70947 Hypothetical protein B0454.1 [Caenorhabditis elegans] gb|AAZ77787.1| synthetic multivulva protein LIN-8 [Caenorhabditis elegans] ref|NP_494436.1| B0454.1 [Caenorhabditis elegans] 5.0E-44
Protein Domains
Database ID Description E-Value
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 7.2E-17
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 2.6E-5
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 1.7E-5
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 2.6E-5
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 7.2E-17
Pfam IPR005020 EMBL-EBI Protein of unknown function DUF278 2.6E-5
Sequence
Target Sequence 
>C. ELEGANS:182123_AT
aagggggagatcatcgctggagaatggtttgacgagtcacagattagacatgcagctgag
ataaatgagatggagaggtcgccggaaggatcagagggaggttcggtggagaaggaagtg
aaggtcgaggcgcctattatggtttatcaggataatagcaccagcctctcccgcactaaa
ggcaatcggcagagctccaaatattatcaacaagacgatgacgatcagccacctccactg
atgcatccaactgaggagtatgcaaaatcggtggttcagcaccgtcagtggattcagaat
ttgggaaaaccccagattgaattgacgccgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGGGGAGATCATCGCTGGAGAAT 76 161 553 Antisense
GGAGAGGTCGCCGGAAGGATCAGAG 52 517 624 Antisense
GGATCAGAGGGAGGTTCGGTGGAGA 431 511 640 Antisense
GTCGAGGCGCCTATTATGGTTTATC 14 475 676 Antisense
TCAGGATAATAGCACCAGCCTCTCC 571 623 699 Antisense
CCTCTCCCGCACTAAAGGCAATCGG 666 265 717 Antisense
GGCAATCGGCAGAGCTCCAAATATT 294 533 733 Antisense
CTCCACTGATGCATCCAACTGAGGA 612 235 785 Antisense
GAGTATGCAAAATCGGTGGTTCAGC 189 397 808 Antisense
GGTTCAGCACCGTCAGTGGATTCAG 392 507 825 Antisense
AACCCCAGATTGAATTGACGCCGAG 328 145 860 Antisense
Sequence Source Affymetrix Proprietary Database