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[182101_at]

Description R09E10.8 /REP_DB=WormBase Gene ID /WP=CE23910 /TR=Q9U391 /GB=CAB54288.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 26      4grp_total > 24      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 7.3
(-2.4)
A 		3.4
(-3)
A 		21.5
(-0.2)
A 		42.8
(3)
A 		39
B 0.8
(-2.9)
A 		0.5
(-3)
A 		20.3
(3)
A 		16
(1.7)
A 		20
C 7.7
(-3)
A 		34.4
(3)
A 		7.7
(-3)
A 		15
(-1.4)
A 		27
avr 5.3
(-3) 		12.8
(-0.7) 		16.5
(0.5) 		24.6
(3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 30.7
(3)
A 		16.7
(0.1)
A 		13.5
(-0.6)
A 		2.2
(-3)
A 		15.3
(-0.2)
A 		19.4
(0.6)
A 		29
B 13.3
(-0.2)
A 		7.6
(-1.6)
A 		7.2
(-1.7)
A 		27.2
(3)
A 		10
(-1)
A 		1.4
(-3)
A 		26
C 2
(-2.7)
A 		27.3
(3)
A 		0.7
(-3)
A 		3.3
(-2.4)
A 		5.9
(-1.8)
A 		15
(0.2)
A 		27
avr 15.3
(1.9) 		17.2
(3) 		7.1
(-3) 		10.9
(-0.7) 		10.4
(-1) 		11.9
(-0.1) 		10
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 2.7
(-0.9)
A 		0.7
(-3)
A 		5.6
(2.3)
A 		6.3
(3)
A 		6
B 0.2
(-2.9)
A 		0.1
(-3)
A 		4.7
(3)
A 		3.4
(1.3)
A 		5
C 2.5
(-3)
A 		9.9
(3)
A 		2.7
(-2.8)
A 		2.5
(-3)
A 		7
avr 1.8
(-3) 		3.6
(1.3) 		4.3
(3) 		4.1
(2.5) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 5.7
(2.8)
A 		5.9
(3)
A 		2.9
(-0.3)
A 		0.5
(-3)
A 		4.6
(1.6)
A 		4.7
(1.7)
A 		5
B 4.6
(0.7)
A 		2.5
(-1.2)
A 		1.8
(-1.8)
A 		7.2
(3)
A 		4.1
(0.2)
A 		0.5
(-3)
A 		7
C 0.7
(-2.6)
A 		8.3
(3)
A 		0.2
(-3)
A 		0.7
(-2.6)
A 		1.9
(-1.7)
A 		5
(0.6)
A 		8
avr 3.7
(0.2) 		5.6
(3) 		1.6
(-3) 		2.8
(-1.2) 		3.5
(-0.2) 		3.4
(-0.3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182101_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10028
Sequence Type Exemplar sequence
Representative Public ID R09E10.8 NCBI
Target Description R09E10.8 /REP_DB=WormBase Gene ID /WP=CE23910 /TR=Q9U391 /GB=CAB54288.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069491(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069491 NCBI Caenorhabditis elegans R09E10.8 (R09E10.8) mRNA, complete cds. 11/11 None
SNAP00000005972 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10310102:10310384:-1 11/11 None
GENEFINDER00000005981 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10306116:10313577:-1 9/11 None
R09E10.8 ENSEMBL cdna:known chromosome:CEL140:IV:10310016:10310384:-1 gene:R09E10.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10310107-10310390(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R09E10.8
Entrez Gene 177917 Entrez gene
SwissProt Q9U391 EMBL-EBI
WormBase CE23910 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94298 Hypothetical protein R09E10.3 [Caenorhabditis elegans] ref|NP_501893.1| R09E10.3 [Caenorhabditis elegans] 0.0
blast CAE60718 Hypothetical protein CBG04390 [Caenorhabditis briggsae] 0.0
blast AAB66234 Hypothetical protein R07C3.4 [Caenorhabditis elegans] ref|NP_493856.1| R07C3.4 [Caenorhabditis elegans] 0.0
blast CAB54288 Hypothetical protein R09E10.8 [Caenorhabditis elegans] ref|NP_501892.1| R09E10.8 [Caenorhabditis elegans] 3.0E-40
blast CAE17782 Hypothetical protein F30A10.11 [Caenorhabditis elegans] ref|NP_001021429.1| F30A10.11 [Caenorhabditis elegans] 3.0E-14
blast CAE63688 Hypothetical protein CBG08194 [Caenorhabditis briggsae] 2.0E-9
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-111
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 5.7E-35
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.3E-40
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.6E-24
Sequence
Target Sequence 
>C. ELEGANS:182101_AT
ttcttcgcccttttggttggatcaaacgcccaatttcaacaattttgcaattactgtgga
ttcattgaagttccaaagacttggccagtagctcaagattctctgagaagtgcatgtgaa
acacttggaatggcaacaacagcgtgcaagggaattattaattcagctgacttgaattca
tca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTTCGCCCTTTTGGTTGGATCAA 493 689 37 Antisense
TCGCCCTTTTGGTTGGATCAAACGC 245 599 41 Antisense
GGATCAAACGCCCAATTTCAACAAT 444 511 55 Antisense
AATTACTGTGGATTCATTGAAGTTC 536 173 85 Antisense
GAAGTTCCAAAGACTTGGCCAGTAG 40 337 103 Antisense
TGGCCAGTAGCTCAAGATTCTCTGA 121 545 118 Antisense
GATTCTCTGAGAAGTGCATGTGAAA 38 433 133 Antisense
GTGCATGTGAAACACTTGGAATGGC 572 477 146 Antisense
GGAATGGCAACAACAGCGTGCAAGG 147 533 163 Antisense
AACAGCGTGCAAGGGAATTATTAAT 8 137 174 Antisense
TAATTCAGCTGACTTGAATTCATCA 38 635 195 Antisense
Sequence Source Affymetrix Proprietary Database