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[182096_at]

Description C01G8.4 /REP_DB=WormBase Gene ID /WP=CE26905 /TR=P91014 /GB=AAB37641.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 21      6grp_cgc > 01      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 48.4
(-0.8)
P 		25.9
(-3)
A 		43.1
(-1.3)
A 		88.1
(3)
A 		62
B 64.3
(3)
A 		62.3
(2.7)
P 		29
(-3)
A 		39.6
(-1.2)
M 		35
C 77.5
(0.5)
P 		62.5
(-1)
P 		42.8
(-3)
P 		101.7
(3)
M 		59
avr 63.4
(0.9) 		50.2
(-1.1) 		38.3
(-3) 		76.5
(3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 70.6
(0.1)
A 		63.2
(-0.9)
P 		46.5
(-3)
A 		93.9
(3)
A 		79.2
(1.1)
P 		62.2
(-1)
A 		47
B 83
(2.2)
M 		39.8
(-3)
P 		89.9
(3)
P 		44.4
(-2.4)
A 		83
(2.2)
A 		66.5
(0.2)
M 		50
C 89.6
(3)
P 		79.1
(0.9)
P 		62.1
(-2.6)
P 		67.4
(-1.5)
A 		74.7
(-0)
P 		59.9
(-3)
M 		30
avr 81.1
(3) 		60.7
(-3) 		66.2
(-1.4) 		68.6
(-0.7) 		79
(2.4) 		62.9
(-2.4) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 17.6
(3)
P 		5.6
(-3)
A 		11.3
(-0.2)
A 		12.9
(0.7)
A 		12
B 20.5
(3)
A 		15.4
(0.8)
P 		6.7
(-3)
A 		8.4
(-2.3)
M 		14
C 25.6
(3)
P 		18
(-1.3)
P 		14.9
(-3)
P 		17.2
(-1.7)
M 		11
avr 21.2
(3) 		13
(-1.8) 		11
(-3) 		12.8
(-1.9) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 13
(-1.7)
A 		22.5
(2.4)
P 		10.1
(-3)
A 		23.8
(3)
A 		23.9
(3)
P 		15.2
(-0.8)
A 		14
B 28.9
(1.7)
M 		13.2
(-2.6)
P 		21.8
(-0.3)
P 		11.8
(-3)
A 		33.7
(3)
A 		24.8
(0.6)
M 		22
C 33.2
(3)
P 		24.1
(0.2)
P 		15.8
(-2.3)
P 		13.5
(-3)
A 		24.2
(0.3)
P 		20
(-1)
M 		20
avr 25
(1.8) 		19.9
(-0.9) 		15.9
(-3) 		16.4
(-2.7) 		27.3
(3) 		20
(-0.8) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182096_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1790
Sequence Type Exemplar sequence
Representative Public ID C01G8.4 NCBI
Target Description C01G8.4 /REP_DB=WormBase Gene ID /WP=CE26905 /TR=P91014 /GB=AAB37641.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059157
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000016692 ENSEMBL cdna:SNAP chromosome:CEL140:I:5282266:5283963:-1 None
GENEFINDER00000016705 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5282266:5283929:-1 None
C01G8.4 ENSEMBL cdna:known chromosome:CEL140:I:5282150:5283946:-1 gene:C01G8.4 None
NM_059157 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-4) (dnj-4) mRNA, complete cds. None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5282335-5283929(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dnj-4
Ensembl C01G8.4
Entrez Gene 172173 Entrez gene
SwissProt P91014 EMBL-EBI
WormBase CE26905 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6457 protein folding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37641 Dnaj domain (prokaryotic heat shock protein) protein 4 [Caenorhabditis elegans] ref|NP_491558.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-4) [Caenorhabditis elegans] 1.0E-114
blast AAB37641 Dnaj domain (prokaryotic heat shock protein) protein 4 [Caenorhabditis elegans] ref|NP_491558.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-4) [Caenorhabditis elegans] 1.0E-104
blast AAB37641 Dnaj domain (prokaryotic heat shock protein) protein 4 [Caenorhabditis elegans] ref|NP_491558.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-4) [Caenorhabditis elegans] 1.0E-102
blast CAE67383 Hypothetical protein CBG12866 [Caenorhabditis briggsae] 2.0E-87
blast CAE67383 Hypothetical protein CBG12866 [Caenorhabditis briggsae] 3.0E-79
blast YP_175156 molecular chaperone DnaJ [Bacillus clausii KSM-K16] dbj|BAD64195.1| molecular chaperone DnaJ [Bacillus clausii KSM-K16] sp|Q5WHG0|DNAJ_BACSK Chaperone protein dnaJ 9.0E-9
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 5.00000001813747E-16
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 4.00000006744953E-16
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 3.0000001167616E-16
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 6.0E-23
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 6.0E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491558.1 1 150-172
C01G8.4 1 150-172
SNAP00000016692 1 154-176
GENEFINDER00000016705 1 150-172
Sequence
Target Sequence 
>C. ELEGANS:182096_AT
atggactgccattggtttagtacttgttgctggatataatggaggatatttatatcttct
ggcttataatcaaaagcaactagacaaattaatcgatgaagatgagattgcaaaatgctt
tttgcggcaaaaagagttccgaaatgctaactttgaaagctcggaggttgctgaaattgg
aagaatattaaaagctgacgttgatgagatttggaagacgaaaacccaaagaaatgaaaa
tgagattcgagaagaatatcggtacggaaggtgtttcgaaattatgagatttttcgctac
aaaatacggtctcgccacgaaaggagtttttctttcaaagtctttcaaaactactgtatt
cgaatttttcgctttttttaaaaattttcctcgtttctttcctctcagccacccaaattt
tacattacaatttttttttcatacgtttcaaaatgatttgatcattttgaaagttggttt
gaaaaacaaatcaggcagcattgaaattaaaattaaattgaattccacgaaattgtattt
tcaggtggttccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGACTGCCATTGGTTTAGTACTT 197 53 468 Antisense
GGTTTAGTACTTGTTGCTGGATATA 481 505 481 Antisense
GGATATTTATATCTTCTGGCTTATA 93 509 511 Antisense
AGAGTTCCGAAATGCTAACTTTGAA 612 61 600 Antisense
GAAAGCTCGGAGGTTGCTGAAATTG 283 359 622 Antisense
GAATATCGGTACGGAAGGTGTTTCG 578 321 721 Antisense
TTCGCTACAAAATACGGTCTCGCCA 454 697 760 Antisense
TACGGTCTCGCCACGAAAGGAGTTT 607 619 772 Antisense
GTCTTTCAAAACTACTGTATTCGAA 247 469 807 Antisense
CTCAGCCACCCAAATTTTACATTAC 86 227 871 Antisense
GAAATTGTATTTTCAGGTGGTTCCG 534 363 996 Antisense
Sequence Source Affymetrix Proprietary Database