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[182089_at]

Description Y53H1A.2 /REP_DB=WormBase Gene ID /WP=CE25432 /TR=Q9U251 /GB=CAB63393.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 20      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 52.5
(0.5)
A 		57
(0.9)
A 		8.6
(-3)
A 		83.9
(3)
M 		75
B 54.8
(0.4)
M 		64.9
(1.2)
P 		12.9
(-3)
A 		86.9
(3)
A 		74
C 60.3
(-0)
A 		32.1
(-3)
A 		44.3
(-1.7)
A 		89.2
(3)
A 		57
avr 55.9
(0.1) 		51.3
(-0.3) 		21.9
(-3) 		86.7
(3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 122
1 2 3 4 5 6 range
A 102.7
(0.9)
P 		68.4
(-1.5)
A 		45.7
(-3)
A 		65.3
(-1.7)
A 		64.6
(-1.7)
M 		134.4
(3)
P 		89
B 57.7
(-2.1)
M 		62.4
(-1.9)
A 		41.4
(-3)
A 		93.1
(-0.3)
A 		139.2
(2.2)
P 		155.2
(3)
P 		114
C 88.5
(0.3)
P 		54.5
(-1.7)
A 		32.9
(-3)
A 		85.6
(0.1)
A 		134.2
(3)
P 		120.3
(2.2)
P 		101
avr 83
(-0.3) 		61.8
(-1.6) 		40
(-3) 		81.3
(-0.4) 		112.7
(1.5) 		136.6
(3) 		97
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 19.1
(3)
A 		12.4
(0.6)
A 		2.2
(-3)
A 		12.3
(0.6)
M 		17
B 17.5
(2.6)
M 		16
(2)
P 		3
(-3)
A 		18.5
(3)
A 		16
C 20
(3)
A 		9.2
(-3)
A 		15.5
(0.5)
A 		15
(0.2)
A 		11
avr 18.9
(3) 		12.5
(-0.2) 		6.9
(-3) 		15.3
(1.2) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 18.9
(-0.6)
P 		24.3
(0.8)
A 		10
(-3)
A 		16.6
(-1.3)
A 		19.5
(-0.5)
M 		32.7
(3)
P 		23
B 20.1
(-1.7)
M 		20.8
(-1.7)
A 		10.1
(-3)
A 		24.6
(-1.2)
A 		56.6
(2.8)
P 		57.9
(3)
P 		48
C 32.8
(1.2)
P 		16.6
(-1.6)
A 		8.4
(-3)
A 		17.2
(-1.5)
A 		43.4
(3)
P 		40.3
(2.5)
P 		35
avr 23.9
(-0.5) 		20.6
(-1) 		9.5
(-3) 		19.5
(-1.2) 		39.8
(2.3) 		43.6
(3) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182089_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2044
Sequence Type Exemplar sequence
Representative Public ID Y53H1A.2 NCBI
Target Description Y53H1A.2 /REP_DB=WormBase Gene ID /WP=CE25432 /TR=Q9U251 /GB=CAB63393.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026616(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026616 NCBI Caenorhabditis elegans Y53H1A.5 (Y53H1A.5) mRNA, complete cds. 11/11 None
GENEFINDER00000030287 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11230633:11241373:1 11/11 None
Y53H1A.5 ENSEMBL cdna:known chromosome:CEL140:I:11232960:11241435:1 gene:Y53H1A.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000030280 5/11 Cross Hyb Matching Probes None
SNAP00000030282 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11230563-11241304(+) 99.55 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53H1A.5
Entrez Gene 3565955 Entrez gene
SwissProt Q5ZEP9 EMBL-EBI
WormBase CE37572 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAH60767 Hypothetical protein Y53H1A.5 [Caenorhabditis elegans] ref|NP_001021787.1| Y53H1A.5 [Caenorhabditis elegans] 1.0E-129
blast CAH60767 Hypothetical protein Y53H1A.5 [Caenorhabditis elegans] ref|NP_001021787.1| Y53H1A.5 [Caenorhabditis elegans] 1.0E-118
blast CAB63393 Hypothetical protein Y53H1A.2 [Caenorhabditis elegans] ref|NP_492879.2| Y53H1A.2 [Caenorhabditis elegans] 1.0E-113
blast CAA90639 Hypothetical protein T08D10.1 [Caenorhabditis elegans] emb|CAA90612.1| Hypothetical protein T08D10.1 [Caenorhabditis elegans] ref|NP_509999.1| Temporarily Assigned Gene name family member (tag-295) [Caenorhabditis elegans] 7.0E-30
blast CAE63484 Hypothetical protein CBG07952 [Caenorhabditis briggsae] 7.0E-28
blast CAE69400 Hypothetical protein CBG15536 [Caenorhabditis briggsae] 3.0E-24
Protein Domains
Database ID Description E-Value
Pfam IPR001289 EMBL-EBI CCAAT-binding transcription factor, subunit B 2.5E-32
Pfam IPR001289 EMBL-EBI CCAAT-binding transcription factor, subunit B 2.5E-32
Sequence
Target Sequence 
>C. ELEGANS:182089_AT
gccggactgcaaactcgtcgaatacacgtggcaagagaacggtgaatctcgaacggtttt
aattccaatgtcgaatgattctacggaagatgacgtcagatcagcacttccccaatcggt
tctagaatcgataaatcagcatcaaaatcagcttagacagggaaatgctcaagcagttca
ttactatcagaagcatcagaatccaatttcacatgaaaaaccgattatggtgaacccaag
acagtacaaaagaatcataaagcgccgagaaatgcgccagaaaatggaagattctggacg
tttgccccttgaacgacagaaatatatgcatgaaagtcgacggcaacacgcgttga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCGGACTGCAAACTCGTCGAATAC 361 283 948 Antisense
GAGAACGGTGAATCTCGAACGGTTT 233 389 982 Antisense
TCGAACGGTTTTAATTCCAATGTCG 702 599 996 Antisense
AGATGACGTCAGATCAGCACTTCCC 645 67 1035 Antisense
ACTTCCCCAATCGGTTCTAGAATCG 36 105 1053 Antisense
TGCTCAAGCAGTTCATTACTATCAG 344 579 1113 Antisense
GAAGCATCAGAATCCAATTTCACAT 35 343 1137 Antisense
ATGGTGAACCCAAGACAGTACAAAA 34 51 1174 Antisense
AAAGCGCCGAGAAATGCGCCAGAAA 97 125 1206 Antisense
TGGAAGATTCTGGACGTTTGCCCCT 471 547 1232 Antisense
GAAAGTCGACGGCAACACGCGTTGA 624 351 1279 Antisense
Sequence Source Affymetrix Proprietary Database