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[182071_at]

Description K12D12.4 /REP_DB=WormBase Gene ID /WP=CE02273 /TR=Q21438 /GB=CAA88866.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 74      4grp_total > 29      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 70
1 2 3 4 range
A 24.2
(-1)
A 		27.3
(-0.6)
A 		5.9
(-3)
A 		60.5
(3)
A 		55
B 75.6
(3)
A 		31.1
(-1.4)
A 		24.5
(-2)
A 		14.7
(-3)
A 		61
C 40.3
(1.6)
A 		30.1
(0.2)
A 		7.1
(-3)
A 		50.8
(3)
A 		44
avr 46.7
(3) 		29.5
(-0) 		12.5
(-3) 		42
(2.2) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 111.3
(3)
A 		57.5
(-2.7)
A 		85.5
(0.3)
A 		99
(1.7)
P 		96.5
(1.4)
P 		54.5
(-3)
M 		57
B 46
(-3)
A 		55.8
(-2)
A 		55
(-2.1)
A 		89.6
(1.5)
A 		85.8
(1.1)
A 		104.7
(3)
P 		59
C 47.7
(-3)
A 		50.6
(-2.8)
A 		104.5
(1.8)
M 		118.1
(3)
A 		59.6
(-2)
A 		105.8
(2)
P 		70
avr 68.3
(-1.3) 		54.6
(-3) 		81.7
(0.4) 		102.2
(3) 		80.6
(0.3) 		88.3
(1.2) 		48
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 8.8
(2.9)
A 		5.9
(0.6)
A 		1.5
(-3)
A 		8.9
(3)
A 		7
B 24.1
(3)
A 		7.7
(-1.7)
A 		5.7
(-2.3)
A 		3.1
(-3)
A 		21
C 13.3
(3)
A 		8.7
(0.4)
A 		2.5
(-3)
A 		8.6
(0.4)
A 		11
avr 15.4
(3) 		7.4
(-0.9) 		3.2
(-3) 		6.9
(-1.2) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 20.5
(-0.3)
A 		20.5
(-0.3)
A 		18.6
(-1)
A 		25.1
(1.5)
P 		29.1
(3)
P 		13.3
(-3)
M 		16
B 16
(-2.4)
A 		18.6
(-1.8)
A 		13.3
(-3)
A 		23.7
(-0.6)
A 		34.9
(2)
A 		39.1
(3)
P 		26
C 17.7
(-2.3)
A 		15.4
(-3)
A 		26.6
(0.4)
M 		23.8
(-0.5)
A 		19.3
(-1.8)
A 		35.4
(3)
P 		20
avr 18.1
(-3) 		18.2
(-2.9) 		19.5
(-2.3) 		24.2
(0.3) 		27.8
(2.2) 		29.3
(3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182071_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2885
Sequence Type Exemplar sequence
Representative Public ID K12D12.4 NCBI
Target Description K12D12.4 /REP_DB=WormBase Gene ID /WP=CE02273 /TR=Q21438 /GB=CAA88866.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NR_002362(9), NM_001027080(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NR_002362 NCBI Caenorhabditis elegans K12D12.4, miscRNA. 9/11 None
NM_001027080 NCBI Caenorhabditis elegans K12D12.4a (K12D12.4) mRNA, complete cds. 9/11 None
SNAP00000036843 ENSEMBL cdna:SNAP chromosome:CEL140:II:11863430:11865231:-1 9/11 None
GENEFINDER00000036848 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11863430:11865361:-1 9/11 None
K12D12.4a ENSEMBL cdna:known chromosome:CEL140:II:11863430:11865361:-1 gene:K12D12.4 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11863426-11865358(-) 100.0 100.0
Public Domain and Genome References
Ensembl K12D12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA88866 Hypothetical protein K12D12.4a [Caenorhabditis elegans] ref|NP_001022251.1| K12D12.4a [Caenorhabditis elegans] 0.0
blast CAA88866 Hypothetical protein K12D12.4a [Caenorhabditis elegans] ref|NP_001022251.1| K12D12.4a [Caenorhabditis elegans] 1.0E-177
blast CAA88866 Hypothetical protein K12D12.4a [Caenorhabditis elegans] ref|NP_001022251.1| K12D12.4a [Caenorhabditis elegans] 1.0E-162
blast CAE59692 Hypothetical protein CBG03116 [Caenorhabditis briggsae] 8.0E-94
blast CAE59692 Hypothetical protein CBG03116 [Caenorhabditis briggsae] 7.0E-89
blast CAE59692 Hypothetical protein CBG03116 [Caenorhabditis briggsae] 1.0E-82
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022251.1 1 54-76
K12D12.4a 1 54-76
SNAP00000036843 1 9-31
GENEFINDER00000036848 1 54-76
Sequence
Target Sequence 
>C. ELEGANS:182071_AT
tgtgccgcccgtattgtaagaaaattgaatttttaagccgagtagagcccgtgttttcag
cccaaaacggaccgaaactgacgtttaaggaatacatggacatcgctgaaatctgctatc
attacgttaggcaaatgacgaggaaggctaaaatttcagcgtttggtaaaggaattgcag
ccaacattctcctggcggctgctggaaatctccgagatgaatattgtttctcgtttgact
atattttcatggaaaatcccatcaatgatgtccgcaaagaatttgaaagcaaaagtttca
ttggaattgcatttatgcttccttggatgcgtgagcagatgataacttccacgattcgag
actttggactagatctggcagagattatggataatctgaagcgagtcaatacgaaggtca
ctgagctagttgtaggcgagaatgcgatctacggaaattatggtggatccgttggaaaag
gacatcggaagaagatctttgagtacatgaaaaagaatccagacccatcggcaaaaaatg
cgacaggcggcttcgagttcagacatcctggcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTGCCGCCCGTATTGTAAGAAAAT 160 557 575 Antisense
TTAAGCCGAGTAGAGCCCGTGTTTT 709 549 607 Antisense
GGACATCGCTGAAATCTGCTATCAT 278 525 672 Antisense
AATTGCAGCCAACATTCTCCTGGCG 23 179 747 Antisense
TGGCGGCTGCTGGAAATCTCCGAGA 528 543 767 Antisense
TTTATGCTTCCTTGGATGCGTGAGC 634 671 886 Antisense
GCAGATGATAACTTCCACGATTCGA 284 313 909 Antisense
GACTTTGGACTAGATCTGGCAGAGA 1 373 934 Antisense
AAGAATCCAGACCCATCGGCAAAAA 20 153 1087 Antisense
AAATGCGACAGGCGGCTTCGAGTTC 255 119 1110 Antisense
GCTTCGAGTTCAGACATCCTGGCGA 56 307 1124 Antisense
Sequence Source Affymetrix Proprietary Database