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[182044_at]

Description H25K10.5 /REP_DB=WormBase Gene ID /WP=CE19937 /TR=Q9U3D2 /GB=CAB63231.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 63      4grp_total > 26      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 2.2
(-3)
A 		14.7
(3)
A 		7.4
(-0.5)
A 		4
(-2.1)
A 		13
B 3.1
(-2.5)
A 		4.4
(-1.8)
A 		14.5
(3)
A 		2
(-3)
A 		13
C 13.5
(-1.5)
A 		18.7
(-0.7)
A 		2.7
(-3)
A 		44.6
(3)
A 		42
avr 6.3
(-3) 		12.6
(0.6) 		8.2
(-1.9) 		16.9
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 4.2
(-2.7)
A 		5.8
(-2.5)
A 		50.4
(3)
A 		15.8
(-1.3)
A 		13.2
(-1.6)
A 		1.6
(-3)
A 		49
B 2.5
(-3)
A 		3.3
(-2.8)
A 		3.7
(-2.7)
A 		23.2
(3)
A 		3.1
(-2.8)
A 		18.3
(1.6)
A 		21
C 2.8
(-2.3)
A 		3.6
(-1.9)
A 		14.3
(3)
A 		3.8
(-1.8)
A 		1.4
(-2.9)
A 		1.2
(-3)
A 		13
avr 3.2
(-3) 		4.2
(-2.7) 		22.8
(3) 		14.3
(0.4) 		5.9
(-2.2) 		7
(-1.8) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 0.8
(-2.5)
A 		3.2
(3)
A 		1.9
(-0)
A 		0.6
(-3)
A 		3
B 1
(-1.8)
A 		1.1
(-1.6)
A 		3.4
(3)
A 		0.4
(-3)
A 		3
C 4.5
(0.3)
A 		5.4
(1.1)
A 		0.9
(-3)
A 		7.5
(3)
A 		7
avr 2.1
(-3) 		3.2
(3) 		2.1
(-3) 		2.8
(0.8) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 0.8
(-2.8)
A 		2.1
(-2)
A 		11
(3)
A 		4
(-1)
A 		4
(-1)
A 		0.4
(-3)
A 		11
B 0.9
(-3)
A 		1.1
(-2.8)
A 		0.9
(-3)
A 		6.1
(2.3)
A 		1.2
(-2.7)
A 		6.8
(3)
A 		6
C 1
(-1.9)
A 		1.1
(-1.7)
A 		3.6
(3)
A 		0.8
(-2.3)
A 		0.4
(-3)
A 		0.4
(-3)
A 		3
avr 0.9
(-3) 		1.4
(-2.3) 		5.2
(3) 		3.6
(0.8) 		1.9
(-1.6) 		2.5
(-0.8) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182044_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8799
Sequence Type Exemplar sequence
Representative Public ID H25K10.5 NCBI
Target Description H25K10.5 /REP_DB=WormBase Gene ID /WP=CE19937 /TR=Q9U3D2 /GB=CAB63231.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070523(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070523 NCBI Caenorhabditis elegans H25K10.5 (H25K10.5) mRNA, complete cds. 11/11 None
SNAP00000037536 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16230723:16232573:-1 9/11 None
GENEFINDER00000037546 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16229425:16233089:-1 10/11 None
H25K10.5 ENSEMBL cdna:known chromosome:CEL140:IV:16230413:16233089:-1 gene:H25K10.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16230418-16233095(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H25K10.5
Entrez Gene 186781 Entrez gene
SwissProt Q9U3D2 EMBL-EBI
WormBase CE19937 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4714 transmembrane receptor protein tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5006 epidermal growth factor receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB63231 Hypothetical protein H25K10.5 [Caenorhabditis elegans] ref|NP_502924.1| H25K10.5 [Caenorhabditis elegans] 0.0
blast CAB63231 Hypothetical protein H25K10.5 [Caenorhabditis elegans] ref|NP_502924.1| H25K10.5 [Caenorhabditis elegans] 1.0E-177
blast AAF39996 Hypothetical protein ZK355.4 [Caenorhabditis elegans] ref|NP_494402.1| ZK355.4 [Caenorhabditis elegans] 4.0E-99
blast CAA19492 Hypothetical protein Y37A1B.9 [Caenorhabditis elegans] ref|NP_502687.1| Y37A1B.9 [Caenorhabditis elegans] 1.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 7.1E-34
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 4.8E-8
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 7.1E-34
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 1.4E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502924.1 1 19-41
H25K10.5 1 19-41
GENEFINDER00000037546 1 19-41
Sequence
Target Sequence 
>C. ELEGANS:182044_AT
ttctgcatcacgtttcgcgaatggaggatatttttggaaaattacgtggaatttcacaac
cttaaagacgcaaagctctgccaagaattggagtcggctcaagaaagtgacttcgttttt
tgcaactttacaagtatggctgttttaccaatcgtttgtcaagttattgtcggtgacgtc
atcattgatgcaaatgacggacagtatttgaaaaaactaatgtacatttcgtatatattc
ggaactctgaaggttcagtacacaaaagcggatgggttggaatttctgacgggactagag
tatattgtctcattggacgaaggaaaagctccaattactatacgctccaacaagtatctc
aaagatgccagactaccatatatcaagcacataatctcaaagaacgagaaatcagtagtg
atatacgacaatccaattttatttgaaaacaacgaacactgcaaaatgtatcagttaact
tttaatacgaatctgaaaattgaaaatggggattgcgagaaaagggtctataagacgtca
catctcctaaatctgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTGCATCACGTTTCGCGAATGGA 188 693 961 Antisense
GGAATTTCACAACCTTAAAGACGCA 633 531 1008 Antisense
GACGCAAAGCTCTGCCAAGAATTGG 51 381 1027 Antisense
GAAAGTGACTTCGTTTTTTGCAACT 142 361 1063 Antisense
GGCTGTTTTACCAATCGTTTGTCAA 13 541 1098 Antisense
GTTATTGTCGGTGACGTCATCATTG 581 447 1123 Antisense
GATGGGTTGGAATTTCTGACGGGAC 360 405 1231 Antisense
AGCTCCAATTACTATACGCTCCAAC 462 81 1287 Antisense
ACGCTCCAACAAGTATCTCAAAGAT 251 91 1302 Antisense
GCCAGACTACCATATATCAAGCACA 692 277 1327 Antisense
AGACGTCACATCTCCTAAATCTGTT 447 69 1493 Antisense
Sequence Source Affymetrix Proprietary Database