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[182029_at]

Description K08A2.2 /REP_DB=WormBase Gene ID /WP=CE21036 /TR=Q9N5J3 /GB=AAF39915.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 29      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 52.6
(-1.7)
A 		49.1
(-2)
A 		38.1
(-3)
A 		103.9
(3)
A 		66
B 47.1
(3)
A 		32.1
(-3)
A 		35
(-1.8)
A 		38.8
(-0.3)
A 		15
C 89.6
(3)
A 		80.9
(2.2)
A 		39.3
(-1.7)
A 		25.9
(-3)
A 		64
avr 63.1
(3) 		54
(0.9) 		37.5
(-3) 		56.2
(1.4) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 110
1 2 3 4 5 6 range
A 62.9
(-1)
A 		34.3
(-2.7)
A 		29.6
(-3)
A 		68.1
(-0.7)
A 		81.9
(0.1)
P 		129.7
(3)
P 		100
B 49.8
(-2.2)
A 		55.2
(-1.6)
A 		53.5
(-1.8)
A 		43.7
(-3)
A 		89.7
(2.7)
A 		91.8
(3)
P 		48
C 48.6
(-1.3)
A 		19.4
(-3)
A 		43.8
(-1.6)
A 		56.5
(-0.8)
A 		75.2
(0.3)
P 		122.4
(3)
P 		103
avr 53.8
(-1.7) 		36.3
(-3) 		42.3
(-2.5) 		56.1
(-1.5) 		82.3
(0.5) 		114.6
(3) 		78
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 19.1
(3)
A 		10.7
(-2.5)
A 		9.9
(-3)
A 		15.2
(0.5)
A 		9
B 15
(3)
A 		7.9
(-3)
A 		8.1
(-2.8)
A 		8.3
(-2.7)
A 		7
C 29.7
(3)
A 		23.3
(1.5)
A 		13.7
(-0.8)
A 		4.4
(-3)
A 		25
avr 21.3
(3) 		14
(-0.7) 		10.6
(-2.4) 		9.3
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 11.6
(-1.8)
A 		12.2
(-1.6)
A 		6.5
(-3)
A 		17.3
(-0.4)
A 		24.7
(1.4)
P 		31.6
(3)
P 		25
B 17.3
(-1.6)
A 		18.4
(-1.4)
A 		13
(-2.7)
A 		11.6
(-3)
A 		36.4
(3)
A 		34.2
(2.5)
P 		25
C 18
(-0.9)
A 		5.9
(-3)
A 		11.2
(-2.1)
A 		11.4
(-2.1)
A 		24.3
(0.1)
P 		41
(3)
P 		35
avr 15.6
(-1.7) 		12.2
(-2.5) 		10.2
(-3) 		13.4
(-2.2) 		28.5
(1.3) 		35.6
(3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182029_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4394
Sequence Type Exemplar sequence
Representative Public ID K08A2.2 NCBI
Target Description K08A2.2 /REP_DB=WormBase Gene ID /WP=CE21036 /TR=Q9N5J3 /GB=AAF39915.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062017(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062017 NCBI Caenorhabditis elegans K08A2.2 (K08A2.2) mRNA, complete cds. 11/11 None
GENEFINDER00000036650 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2991612:2994269:1 11/11 None
K08A2.2 ENSEMBL cdna:known chromosome:CEL140:II:2991612:2994269:1 gene:K08A2.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000036645 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2991610-2994268(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08A2.2
Entrez Gene 173644 Entrez gene
SwissProt Q9N5J3 EMBL-EBI
WormBase CE21036 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39915 Hypothetical protein K08A2.2 [Caenorhabditis elegans] ref|NP_494418.1| K08A2.2 [Caenorhabditis elegans] 0.0
blast CAE72442 Hypothetical protein CBG19612 [Caenorhabditis briggsae] 1.0E-126
blast CAE72442 Hypothetical protein CBG19612 [Caenorhabditis briggsae] 1.0E-123
blast NP_504868 F10G2.7 [Caenorhabditis elegans] gb|AAG24061.1| Hypothetical protein F10G2.7 [Caenorhabditis elegans] 1.0E-110
blast NP_504868 F10G2.7 [Caenorhabditis elegans] gb|AAG24061.1| Hypothetical protein F10G2.7 [Caenorhabditis elegans] 1.0E-107
Protein Domains
Database ID Description E-Value
Pfam IPR009658 EMBL-EBI Protein of unknown function DUF1248 2.2E-126
Pfam IPR009658 EMBL-EBI Protein of unknown function DUF1248 1.4E-118
Sequence
Target Sequence 
>C. ELEGANS:182029_AT
tctcacagattaaccaaccggccaaaaatttctggcacaagctgtccaagcggaaagaat
tcttggatctcggacacaaagccggcgacgttggctacaagacattctacagtgcacacg
acgtcgtgctccctgagaacttggatttgtctggaattacagtgaaaaatgctcgagaag
ttccgaagagagatatcataaattatgatcagacaattcatccgtatcaccgcgaaaagt
acatcatctctcatatgtacgatcgggatggattcgggaaagtggcgtatgatgaggagg
gaaatgtgattggaatcgggcaagctgtgatctatgagaacagaaaagattgtaatttag
ggcctatttatgctgaaaatacacgtgtcgctcaggcaatgtttgcggagattttgaaag
atattaaggcatccgagaagacggtcgtcagttttgaagttcgatccgggcagctgaata
aggatagtttttgctggctcaccccattcctgacacacaaaccaactcgttcgcacgtct
gtaacctggtctacactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCACAGATTAACCAACCGGCCAA 112 611 434 Antisense
TTCTGGCACAAGCTGTCCAAGCGGA 310 695 463 Antisense
GGATCTCGGACACAAAGCCGGCGAC 334 511 498 Antisense
AAGACATTCTACAGTGCACACGACG 516 153 532 Antisense
GTCGTGCTCCCTGAGAACTTGGATT 579 471 556 Antisense
ATCAGACAATTCATCCGTATCACCG 233 29 641 Antisense
ATACACGTGTCGCTCAGGCAATGTT 529 23 812 Antisense
TTTGAAGTTCGATCCGGGCAGCTGA 420 665 886 Antisense
AAGGATAGTTTTTGCTGGCTCACCC 317 159 913 Antisense
TGACACACAAACCAACTCGTTCGCA 455 571 944 Antisense
CACGTCTGTAACCTGGTCTACACTG 462 199 967 Antisense
Sequence Source Affymetrix Proprietary Database