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[182020_s_at]

Description T05A8.5 /REP_DB=WormBase Gene ID /WP=CE27435 /TR=O44856 /GB=AAB95022.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 07      4grp_total > 13      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 63.3
(0.1)
A 		27.7
(-1.9)
A 		7.4
(-3)
A 		116.7
(3)
A 		109
B 52.6
(1)
A 		22.9
(-1.7)
A 		9.1
(-3)
A 		73.9
(3)
A 		65
C 39
(-0.6)
A 		14.2
(-2.7)
A 		11.2
(-3)
A 		80.4
(3)
A 		69
avr 51.6
(0.1) 		21.6
(-2.1) 		9.2
(-3) 		90.3
(3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 68.7
(3)
A 		4.5
(-3)
A 		5.1
(-2.9)
A 		4.2
(-3)
A 		12.5
(-2.2)
A 		4.9
(-2.9)
A 		65
B 14.1
(-1.5)
A 		42.6
(3)
A 		8.1
(-2.5)
A 		10.6
(-2.1)
A 		4.9
(-3)
A 		9.4
(-2.3)
A 		38
C 44.4
(3)
A 		9.5
(-2.2)
A 		4.1
(-3)
A 		6.8
(-2.6)
A 		5.2
(-2.8)
A 		8.2
(-2.4)
A 		40
avr 42.4
(3) 		18.9
(-0.9) 		5.8
(-3) 		7.2
(-2.8) 		7.5
(-2.7) 		7.5
(-2.7) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 23.1
(3)
A 		6
(-1.8)
A 		1.9
(-3)
A 		17.1
(1.3)
A 		21
B 16.8
(3)
A 		5.7
(-1.5)
A 		2.1
(-3)
A 		15.7
(2.6)
A 		15
C 12.9
(2.6)
A 		4.1
(-2.9)
A 		3.9
(-3)
A 		13.6
(3)
A 		10
avr 17.6
(3) 		5.3
(-1.9) 		2.6
(-3) 		15.5
(2.2) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 12.7
(3)
A 		1.6
(-2.7)
A 		1.1
(-3)
A 		1.1
(-3)
A 		3.8
(-1.6)
A 		1.2
(-2.9)
A 		12
B 4.9
(-1.6)
A 		14.2
(3)
A 		2
(-3)
A 		2.8
(-2.6)
A 		2
(-3)
A 		3.5
(-2.3)
A 		12
C 16.5
(3)
A 		2.9
(-2.3)
A 		1
(-3)
A 		1.4
(-2.8)
A 		1.7
(-2.7)
A 		2.7
(-2.3)
A 		16
avr 11.4
(3) 		6.2
(-0.1) 		1.4
(-3) 		1.8
(-2.8) 		2.5
(-2.3) 		2.5
(-2.3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 182020_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4297
Sequence Type Exemplar sequence
Representative Public ID T05A8.5 NCBI
Target Description T05A8.5 /REP_DB=WormBase Gene ID /WP=CE27435 /TR=O44856 /GB=AAB95022.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061943(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061943 NCBI Caenorhabditis elegans T05A8.5 (T05A8.5) mRNA, complete cds. 11/11 None
SNAP00000017324 ENSEMBL cdna:SNAP chromosome:CEL140:II:2730450:2734856:-1 10/11 None
GENEFINDER00000017336 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2731319:2735242:-1 11/11 None
T05A8.5 ENSEMBL cdna:known chromosome:CEL140:II:2731319:2735250:-1 gene:T05A8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2731317-2732950(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T05A8.5
Entrez Gene 173617 Entrez gene
SwissProt O44856 EMBL-EBI
WormBase CE27435 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.5784.1.S1_AT similar to WD-repeat protein 31 cfa
CHICKEN:GGA.17334.1.S1_AT similar to WD repeat domain 31 gga
CHICKEN:GGAAFFX.21244.1.S1_S_AT similar to WD repeat domain 31 gga
CHICKEN:GGAAFFX.22704.1.S1_AT similar to WD repeat domain 31 gga
HU35KSUBD:RC_T85254_AT WD repeat domain 31 hs
HU35KSUBD:RC_AA448186_AT WD repeat domain 31 hs
U133_X3P:HS.193760.0.S1_3P_AT WD repeat domain 31 hs
U133_X3P:HS.133331.0.A1_3P_AT WD repeat domain 31 hs
HG-U133_PLUS_2:229516_AT WD repeat domain 31 hs
HG-U133B:229516_AT WD repeat domain 31 hs
HG-U133_PLUS_2:241919_X_AT WD repeat domain 31 hs
HG-U133B:241919_X_AT WD repeat domain 31 hs
HG-U95B:42222_AT WD repeat domain 31 hs
HG-U95E:76763_AT WD repeat domain 31 hs
MOE430A:1423636_AT WD repeat domain 31 mm
MOUSE430A_2:1423636_AT WD repeat domain 31 mm
MOUSE430_2:1423636_AT WD repeat domain 31 mm
MG-U74CV2:167207_AT WD repeat domain 31 mm
MOE430B:1459664_AT WD repeat domain 31, mRNA (cDNA clone MGC:46809 IMAGE:5364307) mm
MOUSE430_2:1459664_AT WD repeat domain 31, mRNA (cDNA clone MGC:46809 IMAGE:5364307) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494344 T05A8.5 [Caenorhabditis elegans] gb|AAB95022.2| Hypothetical protein T05A8.5 [Caenorhabditis elegans] 0.0
blast C88082 protein T05A8.5 [imported] - Caenorhabditis elegans 0.0
blast CAE72407 Hypothetical protein CBG19566 [Caenorhabditis briggsae] 1.0E-145
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.5E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.3E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.0E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.5E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.3E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.0E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.0E-7
Sequence
Target Sequence 
>C. ELEGANS:182020_S_AT
agaccatcaatcgtaacctcgtcacccatatggcctacaacggaaacttggtggctcaga
cgtcggaggacaagagcgtcaggttgtgggatccgcgtaacgcgtcagtggtgaccgaat
tcccgcgaaaacggcacattcagatgttctgcgagtttgacggtgcgaatcgattcttct
cctgctcaaacgggttcaacaatgacgggtgtgagattacgtcctacgacgtccgcaacc
cccgccagtccagagaagcccgcggtcacgagggcaacgtcacctcgctctccatagtgc
aaatggaccagaccaagcggctcctagcttcagtctcggctgacaggcagatcaggctat
ggaagattgaagaggacgggcaggctgtgctaaaaccaatctggaacgaggacgtgccac
tggaagcggataatcttcaagtctgcagctatccggatggacacataattgtgtcgggtg
gcaaaggccgtctcatccactatcaggcgaagctcacggcgcagcgtgtcatcctagacg
tccagctcatccagcagcacagtatggcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACCATCAATCGTAACCTCGTCAC 156 71 470 Antisense
GTCACCCATATGGCCTACAACGGAA 663 463 490 Antisense
GAGCGTCAGGTTGTGGGATCCGCGT 368 385 543 Antisense
GATGTTCTGCGAGTTTGACGGTGCG 161 411 612 Antisense
TGACGGTGCGAATCGATTCTTCTCC 7 571 627 Antisense
TTCTCCTGCTCAAACGGGTTCAACA 221 693 646 Antisense
CGGGTGTGAGATTACGTCCTACGAC 194 241 675 Antisense
TAGCTTCAGTCTCGGCTGACAGGCA 444 649 794 Antisense
ATCTTCAAGTCTGCAGCTATCCGGA 159 31 902 Antisense
GTCTCATCCACTATCAGGCGAAGCT 587 469 959 Antisense
GCTCATCCAGCAGCACAGTATGGCC 352 301 1014 Antisense
Sequence Source Affymetrix Proprietary Database