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[182016_at]

Description C27C12.4 /REP_DB=WormBase Gene ID /WP=CE05321 /TR=Q18251 /GB=CAA93741.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 70      4grp_highest_8_8 > 0      4grp_high_8_8 > 45      6grp_highest_nonorm > 71      6grp_total > 60      4grp_total > 43      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 217.9
(-3)
P 		334.2
(3)
P 		279
(0.2)
P 		235.5
(-2.1)
P 		116
B 258.1
(1.3)
P 		291.4
(3)
P 		201.7
(-1.6)
P 		174.4
(-3)
P 		117
C 217.8
(-3)
P 		312.3
(3)
P 		269.1
(0.3)
P 		232.7
(-2.1)
P 		95
avr 231.3
(-2) 		312.6
(3) 		249.9
(-0.8) 		214.2
(-3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 348
1 2 3 4 5 6 range
A 481.6
(3)
P 		241.2
(-2.8)
P 		318.3
(-1)
P 		342.4
(-0.4)
P 		322.6
(-0.9)
P 		234
(-3)
P 		248
B 271.9
(-1)
P 		319.7
(0.6)
P 		260.1
(-1.4)
P 		389.1
(3)
P 		297.5
(-0.2)
P 		215.1
(-3)
P 		174
C 251.2
(-2.2)
P 		281.2
(-1.7)
P 		282
(-1.7)
P 		554.4
(3)
P 		232.6
(-2.6)
P 		206.6
(-3)
P 		348
avr 334.9
(0.3) 		280.7
(-1.2) 		286.8
(-1.1) 		428.6
(3) 		284.2
(-1.1) 		218.6
(-3) 		210
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 79.3
(3)
P 		72.5
(2.1)
P 		72.9
(2.1)
P 		34.5
(-3)
P 		45
B 82.3
(3)
P 		72
(1.6)
P 		46.7
(-1.7)
P 		37.2
(-3)
P 		45
C 72.1
(0.6)
P 		89.8
(2.6)
P 		93.8
(3)
P 		39.2
(-3)
P 		55
avr 77.9
(3) 		78.1
(3) 		71.1
(2) 		37
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 88.8
(1.7)
P 		85.8
(1.3)
P 		69.3
(-1.2)
P 		86.8
(1.4)
P 		97.3
(3)
P 		57
(-3)
P 		40
B 94.6
(0.3)
P 		106.4
(1.5)
P 		63.2
(-3)
P 		103.1
(1.1)
P 		120.9
(3)
P 		80.2
(-1.2)
P 		58
C 93.1
(0.4)
P 		85.8
(-0.6)
P 		71.8
(-2.6)
P 		111.5
(3)
P 		75.3
(-2.1)
P 		69.2
(-3)
P 		42
avr 92.2
(1.5) 		92.7
(1.6) 		68.1
(-3) 		100.5
(3) 		97.8
(2.5) 		68.8
(-2.9) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182016_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17848
Sequence Type Exemplar sequence
Representative Public ID C27C12.4 NCBI
Target Description C27C12.4 /REP_DB=WormBase Gene ID /WP=CE05321 /TR=Q18251 /GB=CAA93741.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078064(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078064 NCBI Caenorhabditis elegans C27C12.4 (C27C12.4) mRNA, complete cds. 11/11 None
SNAP00000037257 ENSEMBL cdna:SNAP chromosome:CEL140:X:14854071:14856352:1 11/11 None
GENEFINDER00000037262 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14854071:14856456:1 11/11 None
C27C12.4 ENSEMBL cdna:known chromosome:CEL140:X:14854071:14856352:1 gene:C27C12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14854070-14856352(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C27C12.4
Entrez Gene 182962 Entrez gene
SwissProt Q18251 EMBL-EBI
WormBase CE33976 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93741 Hypothetical protein C27C12.4 [Caenorhabditis elegans] ref|NP_510465.2| C27C12.4 [Caenorhabditis elegans] 0.0
blast CAA93741 Hypothetical protein C27C12.4 [Caenorhabditis elegans] ref|NP_510465.2| C27C12.4 [Caenorhabditis elegans] 1.0E-178
blast CAE63239 Hypothetical protein CBG07602 [Caenorhabditis briggsae] 1.0E-168
blast CAE63239 Hypothetical protein CBG07602 [Caenorhabditis briggsae] 1.0E-162
blast CAE63239 Hypothetical protein CBG07602 [Caenorhabditis briggsae] 1.0E-130
blast CAE69879 Hypothetical protein CBG16219 [Caenorhabditis briggsae] 3.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 7.0E-89
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 2.1E-87
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510465.2 12 5-27,50-72,79-98,103-125,138-160,170-192,238-260,280-302,315-337,347-369,389-411,415-437
C27C12.4 12 5-27,50-72,79-98,103-125,138-160,170-192,238-260,280-302,315-337,347-369,389-411,415-437
SNAP00000037257 11 5-27,50-72,79-98,103-125,138-160,170-192,240-262,282-304,317-339,349-371,399-421
GENEFINDER00000037262 11 5-22,37-59,72-91,96-118,131-153,163-185,231-253,273-295,307-324,339-358,379-401
Sequence
Target Sequence 
>C. ELEGANS:182016_AT
gtctatacatcatgcttgtctgccacacttccattgaaaatcttctcggatttggtgatt
ccttacaacgcactttccattggagctggacaaatagccggcggagttgtcaccggtcca
ctaatgaagattctaagaatgcgtagccagcatatcatctacttggcatttgttgggcat
ctgacgtcattcatcttggcctacttgtgcctgccttacgattcgacagttcattccacg
gatgcttcaacatacttggcacctacactcaatctcactttaattatctccttccttctc
ggagtgtcggatgcgttttggcaaactcaaatttatgttacaatcgggcaggtgtttaag
gaggatccagtaaatgcatttgcaattttcaaattcttccagtcaatggcggcatgtgtc
tcattcttctacagctccttcttgttccttccttcacagctattgattctcacaattgga
tgtgttgcttcgacatttttcttcctgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTATACATCATGCTTGTCTGCCA 336 469 778 Antisense
GTCTGCCACACTTCCATTGAAAATC 509 471 795 Antisense
TCCTTACAACGCACTTTCCATTGGA 585 591 837 Antisense
ATAGCCGGCGGAGTTGTCACCGGTC 624 21 871 Antisense
TTTGTTGGGCATCTGACGTCATTCA 432 667 946 Antisense
CATTCATCTTGGCCTACTTGTGCCT 208 217 965 Antisense
TGCCTGCCTTACGATTCGACAGTTC 302 583 985 Antisense
TCAACATACTTGGCACCTACACTCA 182 631 1024 Antisense
AATCTCACTTTAATTATCTCCTTCC 107 171 1048 Antisense
TTCCTTCCTTCACAGCTATTGATTC 166 701 1222 Antisense
TTGCTTCGACATTTTTCTTCCTGAA 70 705 1262 Antisense
Sequence Source Affymetrix Proprietary Database