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[182008_at]

Description R05D11.6 /REP_DB=WormBase Gene ID /WP=CE06241 /TR=Q21738 /GB=CAA99893.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 57      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 74      6grp_total > 57      4grp_total > 54      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 239
1 2 3 4 range
A 590.9
(3)
P 		548.6
(1.8)
P 		423.8
(-1.6)
P 		372.7
(-3)
P 		218
B 476.8
(3)
P 		438.8
(1.2)
P 		415.1
(0)
P 		352.1
(-3)
P 		125
C 591.1
(3)
P 		369
(-3)
P 		473.3
(-0.2)
P 		374.8
(-2.8)
P 		222
avr 552.9
(3) 		452.1
(-0.2) 		437.4
(-0.7) 		366.5
(-3) 		186

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 399
1 2 3 4 5 6 range
A 733
(3)
P 		562.6
(-1.4)
P 		593.4
(-0.6)
P 		499.6
(-3)
P 		706.3
(2.3)
P 		724.8
(2.8)
P 		233
B 735
(0.1)
P 		588.8
(-2.5)
P 		821.5
(1.7)
P 		647.5
(-1.4)
P 		560.6
(-3)
P 		894.2
(3)
P 		334
C 610.4
(-0)
P 		668.2
(1.4)
P 		611.2
(-0)
P 		626.8
(0.4)
P 		495.1
(-3)
P 		729.4
(3)
P 		234
avr 692.8
(0.2) 		606.5
(-2.4) 		675.4
(-0.3) 		591.3
(-2.9) 		587.3
(-3) 		782.8
(3) 		196
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 161
1 2 3 4 range
A 215.1
(3)
P 		119.1
(-0.6)
P 		110.8
(-0.9)
P 		54.5
(-3)
P 		161
B 152
(3)
P 		108.4
(-0.4)
P 		96.2
(-1.3)
P 		75
(-3)
P 		77
C 195.7
(3)
P 		106.1
(-1.1)
P 		165
(1.6)
P 		63.2
(-3)
P 		133
avr 187.6
(3) 		111.2
(-0.7) 		124
(-0.1) 		64.2
(-3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 207
1 2 3 4 5 6 range
A 135.1
(-2.4)
P 		200.1
(2.1)
P 		129.2
(-2.8)
P 		126.7
(-3)
P 		213.1
(3)
P 		176.5
(0.5)
P 		86
B 255.7
(0.1)
P 		196
(-2.1)
P 		199.5
(-2)
P 		171.5
(-3)
P 		227.8
(-0.9)
P 		333.4
(3)
P 		162
C 226.2
(2.1)
P 		203.9
(1)
P 		155.6
(-1.5)
P 		126
(-3)
P 		160.2
(-1.3)
P 		244.2
(3)
P 		118
avr 205.7
(0.5) 		200
(0.2) 		161.4
(-1.9) 		141.4
(-3) 		200.4
(0.2) 		251.4
(3) 		110
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182008_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1338
Sequence Type Exemplar sequence
Representative Public ID R05D11.6 NCBI
Target Description R05D11.6 /REP_DB=WormBase Gene ID /WP=CE06241 /TR=Q21738 /GB=CAA99893.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059924(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059924 NCBI Caenorhabditis elegans R05D11.6 (R05D11.6) mRNA, complete cds. 11/11 None
SNAP00000034020 ENSEMBL cdna:SNAP chromosome:CEL140:I:8604463:8606550:1 11/11 None
GENEFINDER00000034031 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8604463:8608858:1 10/11 None
R05D11.6 ENSEMBL cdna:known chromosome:CEL140:I:8604458:8606672:1 gene:R05D11.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8604393-8606481(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R05D11.6
Entrez Gene 187613 Entrez gene
SwissProt Q21738 EMBL-EBI
WormBase CE32473 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA99893 Hypothetical protein R05D11.6 [Caenorhabditis elegans] ref|NP_492325.2| R05D11.6 [Caenorhabditis elegans] 0.0
blast CAA99891 Hypothetical protein R05D11.4 [Caenorhabditis elegans] ref|NP_492326.1| R05D11.4 [Caenorhabditis elegans] 0.0
blast CAE67097 Hypothetical protein CBG12508 [Caenorhabditis briggsae] 0.0
blast CAA99893 Hypothetical protein R05D11.6 [Caenorhabditis elegans] ref|NP_492325.2| R05D11.6 [Caenorhabditis elegans] 1.0E-175
blast CAE67098 Hypothetical protein CBG12509 [Caenorhabditis briggsae] 3.0E-83
blast CAE67098 Hypothetical protein CBG12509 [Caenorhabditis briggsae] 2.0E-78
blast XP_395328 PREDICTED: similar to CG31301-PA [Apis mellifera] 2.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 8.3E-32
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.1E-8
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.7E-44
Sequence
Target Sequence 
>C. ELEGANS:182008_AT
aaggcatctcttgcaatgacacctcaccatctaacgggcgaacaaatgatgaaaactgcc
acgaatagttgtctcatgaagtggcacgtgaacaagattaaccagaaaattgaaataact
atcgatcgatatgtggccttcagacatacattctcccaatactgtgttgatccaagggat
tgtgcaatcaatactctaattgaaagcattctttcgtgtgacgccgctgttcacgaagaa
agctacgaaattagatttgatggagttccggtggacgagtgttacgctaaatcagtcacc
agaagacttgcaaaaattaaatcagcagtttctaatggtcttacaacatatttggatgca
gttaacatgtcaatgattcaaaatactcaaaaattggaaggatggtctcaacaacttgat
ctcgtcactgccgatcttcttctatcttctcgtgttttatccagcactgaatgcactaaa
ccagcgatggcaacgatcgcgaatcaaatgagcgaagacgtgtgtcaactgattctcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGCATCTCTTGCAATGACACCTC 395 161 406 Antisense
CCTCACCATCTAACGGGCGAACAAA 209 269 427 Antisense
CTGCCACGAATAGTTGTCTCATGAA 161 237 461 Antisense
CTATCGATCGATATGTGGCCTTCAG 686 219 524 Antisense
TGGCCTTCAGACATACATTCTCCCA 3 545 539 Antisense
CATTCTCCCAATACTGTGTTGATCC 318 217 554 Antisense
TTTCGTGTGACGCCGCTGTTCACGA 213 671 617 Antisense
GTTCCGGTGGACGAGTGTTACGCTA 224 441 670 Antisense
TCAACAACTTGATCTCGTCACTGCC 37 631 813 Antisense
CTATCTTCTCGTGTTTTATCCAGCA 566 223 847 Antisense
AGACGTGTGTCAACTGATTCTCAAC 41 71 921 Antisense
Sequence Source Affymetrix Proprietary Database