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[182000_at]

Description W03D2.3 /REP_DB=WormBase Gene ID /WP=CE14510 /TR=O02117 /GB=AAC48254.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 30      4grp_total > 10      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 2.1
(-3)
A 		25.5
(3)
A 		25.5
(3)
A 		24.7
(2.8)
A 		23
B 13.8
(-2.2)
A 		10.8
(-2.7)
A 		8.3
(-3)
A 		51.7
(3)
A 		43
C 17.5
(0)
A 		11.9
(-1.1)
A 		3.2
(-3)
A 		31.4
(3)
A 		28
avr 11.1
(-3) 		16.1
(-1.8) 		12.3
(-2.7) 		35.9
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 12.4
(-1.4)
A 		42
(3)
A 		2
(-3)
A 		26.9
(0.7)
A 		2.5
(-2.9)
A 		3.2
(-2.8)
A 		40
B 3.6
(-2.8)
A 		31.1
(0.7)
A 		34.5
(1.1)
A 		49.3
(3)
A 		8.7
(-2.1)
A 		1.8
(-3)
A 		48
C 2.6
(-3)
A 		23.9
(3)
A 		16.1
(0.8)
A 		10.1
(-0.9)
A 		3.9
(-2.6)
A 		3
(-2.9)
A 		21
avr 6.2
(-2.3) 		32.3
(3) 		17.5
(0) 		28.8
(2.3) 		5
(-2.5) 		2.7
(-3) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 0.8
(-3)
A 		5.5
(1.8)
A 		6.7
(3)
A 		3.6
(-0.2)
A 		6
B 4.4
(-1.4)
A 		2.7
(-2.5)
A 		1.9
(-3)
A 		11
(3)
A 		9
C 5.8
(3)
A 		3.4
(-0.1)
A 		1.1
(-3)
A 		5.3
(2.4)
A 		5
avr 3.7
(-2.1) 		3.9
(-1.8) 		3.2
(-3) 		6.6
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 2.3
(-2.2)
A 		14.9
(3)
A 		0.4
(-3)
A 		6.8
(-0.4)
A 		0.8
(-2.8)
A 		0.8
(-2.8)
A 		15
B 1.3
(-2.7)
A 		10.3
(1.6)
A 		8.4
(0.7)
A 		13.1
(3)
A 		3.5
(-1.6)
A 		0.7
(-3)
A 		12
C 0.9
(-3)
A 		7.3
(3)
A 		4.1
(0)
A 		2
(-2)
A 		1.3
(-2.6)
A 		1
(-2.9)
A 		6
avr 1.5
(-2.6) 		10.8
(3) 		4.3
(-0.9) 		7.3
(0.9) 		1.9
(-2.3) 		0.8
(-3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 182000_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10390
Sequence Type Exemplar sequence
Representative Public ID W03D2.3 NCBI
Target Description W03D2.3 /REP_DB=WormBase Gene ID /WP=CE14510 /TR=O02117 /GB=AAC48254.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068067(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068067 NCBI Caenorhabditis elegans W03D2.3 (W03D2.3) mRNA, complete cds. 11/11 None
W03D2.3 ENSEMBL cdna:known chromosome:CEL140:IV:4052349:4052673:1 gene:W03D2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4052354-4052679(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W03D2.3
Entrez Gene 189152 Entrez gene
SwissProt O02117 EMBL-EBI
WormBase CE14510 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48254 Hypothetical protein W03D2.3 [Caenorhabditis elegans] ref|NP_500468.1| W03D2.3 [Caenorhabditis elegans] 5.0E-31
Sequence
Target Sequence 
>C. ELEGANS:182000_AT
gccgcctcatcaaagtcgcgatgaaatgttgttcgctggtcattcggcgtggagatgaga
cagcgtttgaaacccggagagtgtgtgtgtgttgccgaggtgttagaaaatggagtgacg
aggagatggaggacggaaaagggtggcaagtatataagaaaaacaattggtgtgtgcaag
gagcagaggaggagaagctcgaaaagttcaagctctccaaaaaggct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCGCCTCATCAAAGTCGCGATGAA 89 287 26 Antisense
CGATGAAATGTTGTTCGCTGGTCAT 544 249 44 Antisense
TTCGCTGGTCATTCGGCGTGGAGAT 617 695 57 Antisense
TCGGCGTGGAGATGAGACAGCGTTT 297 607 69 Antisense
GACAGCGTTTGAAACCCGGAGAGTG 90 367 84 Antisense
GGAGAGTGTGTGTGTGTTGCCGAGG 323 519 101 Antisense
GTGTTGCCGAGGTGTTAGAAAATGG 296 485 114 Antisense
GGAGTGACGAGGAGATGGAGGACGG 165 515 137 Antisense
GAGGACGGAAAAGGGTGGCAAGTAT 265 403 154 Antisense
AACAATTGGTGTGTGCAAGGAGCAG 8 115 187 Antisense
AAAGTTCAAGCTCTCCAAAAAGGCT 294 119 228 Antisense
Sequence Source Affymetrix Proprietary Database