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[181992_s_at]

Description R13H8.2 /REP_DB=WormBase Gene ID /WP=CE12820 /TR=O44755 /GB=AAB96742.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 38      4grp_total > 12      6grp_cgc > 18      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 458
1 2 3 4 range
A 279.1
(0.4)
P 		154.5
(-2.5)
A 		132.8
(-3)
A 		391.3
(3)
P 		259
B 235.9
(-1.8)
P 		163.7
(-2.8)
A 		147.5
(-3)
P 		590.4
(3)
P 		443
C 272.3
(-0.3)
P 		242.5
(-1)
M 		145.1
(-3)
P 		432.3
(3)
P 		287
avr 262.4
(-0.8) 		186.9
(-2.2) 		141.8
(-3) 		471.3
(3) 		330

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 259
1 2 3 4 5 6 range
A 165.2
(-0.6)
A 		257.5
(2.9)
P 		102.1
(-3)
A 		143.7
(-1.4)
P 		124.1
(-2.2)
A 		261.4
(3)
P 		159
B 150.3
(3)
P 		132.1
(1.3)
A 		85.4
(-3)
A 		85.4
(-3)
A 		150.1
(3)
M 		138.5
(1.9)
P 		65
C 344.4
(3)
P 		159
(-1.8)
A 		123.5
(-2.7)
M 		112.8
(-3)
A 		168.2
(-1.6)
P 		197.6
(-0.8)
P 		232
avr 220
(3) 		182.9
(1.1) 		103.7
(-3) 		114
(-2.5) 		147.5
(-0.7) 		199.2
(1.9) 		116
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 101.6
(3)
P 		33.5
(-3)
A 		34.7
(-2.9)
A 		57.3
(-0.9)
P 		68
B 75.2
(-0.3)
P 		40.4
(-2.6)
A 		34.2
(-3)
P 		125.8
(3)
P 		92
C 90.1
(3)
P 		69.7
(-0.1)
M 		50.6
(-3)
P 		72.9
(0.4)
P 		40
avr 89
(3) 		47.9
(-2) 		39.8
(-3) 		85.3
(2.5) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 30.4
(-2.3)
A 		91.6
(3)
P 		22.2
(-3)
A 		36.4
(-1.8)
P 		37.5
(-1.7)
A 		63.6
(0.6)
P 		69
B 52.3
(1.7)
P 		44
(0.5)
A 		20.7
(-3)
A 		22.6
(-2.7)
A 		61
(3)
M 		51.6
(1.6)
P 		40
C 127.6
(3)
P 		48.5
(-1.5)
A 		31.4
(-2.5)
M 		22.7
(-3)
A 		54.4
(-1.2)
P 		66.2
(-0.5)
P 		105
avr 70.1
(3) 		61.4
(1.8) 		24.8
(-3) 		27.2
(-2.7) 		51
(0.5) 		60.5
(1.7) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181992_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2491
Sequence Type Exemplar sequence
Representative Public ID R13H8.2 NCBI
Target Description R13H8.2 /REP_DB=WormBase Gene ID /WP=CE12820 /TR=O44755 /GB=AAB96742.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026424(11), NM_001026423(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026424 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
NM_001026423 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
GENEFINDER00000030177 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10765049:10773602:1 11/11 None
R13H8.1b ENSEMBL cdna:known chromosome:CEL140:I:10770891:10784824:1 gene:R13H8.1 11/11 A 
R13H8.1c ENSEMBL cdna:known chromosome:CEL140:I:10770891:10784821:1 gene:R13H8.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026427 7/11 Cross Hyb Matching Probes None
NM_001026425 7/11 Cross Hyb Matching Probes None
NM_001026426 1/11 Cross Hyb Matching Probes None
SNAP00000030173 7/11 Cross Hyb Matching Probes None
SNAP00000030174 4/11 Cross Hyb Matching Probes None
R13H8.1d.2 7/11 Cross Hyb Matching Probes A 
R13H8.1d.1 7/11 Cross Hyb Matching Probes A 
R13H8.1e.2 1/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172981 6 2 NM_001026422 175823_s_at (11)
NM_001026423 175678_at (11), 175823_s_at (11), 181992_s_at (11)
NM_001026424 175678_at (11), 175823_s_at (11), 181992_s_at (11)
NM_001026427 175678_at (11), 175823_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10771154-10773467(+) 100.0 100.0
Public Domain and Genome References
Ensembl R13H8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001021595 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] gb|AAK82919.1| Abnormal dauer formation protein 16, isoform c [Caenorhabditis elegans] gb|AAC47803.1| DAF-16 [Caenorhabditis elegans] gb|AAB84390.1| fork head-related transcription factor DAF-16a1 [Caenorhabditis elegans] 0.0
blast NP_001021594 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] gb|AAK82918.1| Abnormal dauer formation protein 16, isoform b [Caenorhabditis elegans] gb|AAB84391.1| fork head-related transcription factor DAF-16a2 [Caenorhabditis elegans] 0.0
blast NP_001021596 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 8.968310171678829E-44
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 9.00000002853969E-29
Pfam IPR001766 EMBL-EBI Fork head transcription factor 8.7E-23
Pfam IPR001766 EMBL-EBI Fork head transcription factor 6.2E-23
Pfam IPR001766 EMBL-EBI Fork head transcription factor 1.3E-11
Sequence
Target Sequence 
>C. ELEGANS:181992_S_AT
gatatggaaccgattcctcgtgatcggtgcaatacgtggccaatgcgtaggccgcaactc
gaaccaccactcaactcgagtcccattattcatgaacaaattcctgaagaagatgctgac
ctatacgggagcaatgagcaatgtggacagctcggcggagcatcttcaaacgggtcgaca
gcaatgcttcatactccagatggaagcaattctcatcagacatcgtttccttcggaaatg
tccgaatcgccagacgataccgtatcgggaaaaaagacaacgaccagacggaacgcttgg
ggaaatatgtcatatgctgaacttatcactacagccattatggctagtccagagaaacgg
ttaactcttgcacaagtttacgaatggatggtccagaatgttccatacttcagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATATGGAACCGATTCCTCGTGATC 276 423 139 Antisense
GTGATCGGTGCAATACGTGGCCAAT 519 483 158 Antisense
AATGCGTAGGCCGCAACTCGAACCA 331 165 180 Antisense
CTCAACTCGAGTCCCATTATTCATG 39 229 208 Antisense
GAGCATCTTCAAACGGGTCGACAGC 604 387 296 Antisense
GACAGCAATGCTTCATACTCCAGAT 86 369 315 Antisense
TCTCATCAGACATCGTTTCCTTCGG 357 611 349 Antisense
GTCCGAATCGCCAGACGATACCGTA 687 475 378 Antisense
GACAACGACCAGACGGAACGCTTGG 318 365 414 Antisense
AACTTATCACTACAGCCATTATGGC 546 139 458 Antisense
GGTCCAGAATGTTCCATACTTCAGG 625 501 528 Antisense
Sequence Source Affymetrix Proprietary Database