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[181986_at]

Description R12B2.3 /REP_DB=WormBase Gene ID /WP=CE01366 /TR=Q21953 /GB=AAA50741.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 21      6grp_total > 65      4grp_total > 19      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 23.8
(-2.4)
P 		67.4
(0.4)
P 		13.5
(-3)
A 		108.8
(3)
P 		95
B 16.9
(-3)
A 		48.3
(3)
A 		20.5
(-2.3)
A 		33.3
(0.1)
A 		31
C 53.9
(-1)
A 		23.9
(-3)
A 		28.1
(-2.7)
P 		114.5
(3)
P 		91
avr 31.5
(-2) 		46.5
(-0.6) 		20.7
(-3) 		85.5
(3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 28.3
(-0.5)
A 		2.6
(-3)
A 		54.9
(2)
P 		21.8
(-1.2)
A 		65.2
(3)
P 		51.5
(1.7)
P 		63
B 34.5
(-0)
A 		43.2
(2.7)
P 		25.1
(-3)
A 		41
(2)
A 		32.5
(-0.7)
P 		44
(3)
P 		19
C 23.8
(-2.1)
A 		59.2
(3)
P 		47.2
(1.3)
P 		17.9
(-3)
A 		18.9
(-2.9)
M 		42.3
(0.5)
P 		41
avr 28.9
(-2.4) 		35
(-0.4) 		42.4
(1.9) 		26.9
(-3) 		38.9
(0.8) 		45.9
(3) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 8.7
(-0.5)
P 		14.6
(2.4)
P 		3.5
(-3)
A 		15.9
(3)
P 		12
B 5.4
(-2.5)
A 		11.9
(3)
A 		4.8
(-3)
A 		7.1
(-1.1)
A 		7
C 17.8
(2.3)
A 		6.9
(-3)
A 		9.8
(-1.6)
P 		19.3
(3)
P 		12
avr 10.6
(0.4) 		11.1
(0.8) 		6
(-3) 		14.1
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 5.2
(-1.6)
A 		0.9
(-3)
A 		12
(0.5)
P 		5.5
(-1.5)
A 		19.7
(3)
P 		12.5
(0.7)
P 		19
B 12
(0.4)
A 		14.4
(1.8)
P 		6.1
(-3)
A 		10.9
(-0.2)
A 		13.2
(1.1)
P 		16.4
(3)
P 		10
C 8.8
(-0.8)
A 		18.1
(3)
P 		12
(0.5)
P 		3.6
(-3)
A 		6.1
(-2)
M 		14.2
(1.4)
P 		15
avr 8.7
(-1.4) 		11.1
(0.4) 		10
(-0.4) 		6.7
(-3) 		13
(1.9) 		14.4
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181986_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6571
Sequence Type Exemplar sequence
Representative Public ID R12B2.3 NCBI
Target Description R12B2.3 /REP_DB=WormBase Gene ID /WP=CE01366 /TR=Q21953 /GB=AAA50741.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065853(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065853 NCBI Caenorhabditis elegans R12B2.3 (R12B2.3) mRNA, complete cds. 11/11 None
R12B2.3 ENSEMBL cdna:known chromosome:CEL140:III:5805048:5806886:1 gene:R12B2.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000015242 7/11 Cross Hyb Matching Probes None
GENEFINDER00000015251 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5805046-5806885(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R12B2.3
Entrez Gene 187836 Entrez gene
WormBase CE01366 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498254 R12B2.3 [Caenorhabditis elegans] 2.0E-73
blast AAA50741 Hypothetical protein R12B2.3 [Caenorhabditis elegans] 4.0E-36
blast CAE64397 Hypothetical protein CBG09087 [Caenorhabditis briggsae] 9.0E-21
Sequence
Target Sequence 
>C. ELEGANS:181986_AT
tcttttcattcccaggctctagaaatttcacaccaatttacttttcagattttataattt
tcagaccattagacctttcgggtaagagattagttcgaccagatgattcaaatggtacaa
gatcttccactgatgatgtacattcgatgtgctctcagcaaaatgtcaaagtcccaattg
caccaacatctgctcaaatgacattgaacaaagttaaggatccgggaccgtatgagaata
agctggctctgaaagttccgaagaatacgacagtcgatgtggcggctcaaaatcttgccg
agcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTTTCATTCCCAGGCTCTAGAAA 188 615 38 Antisense
TTCACACCAATTTACTTTTCAGATT 135 685 64 Antisense
TTTTCAGACCATTAGACCTTTCGGG 113 675 95 Antisense
GAGATTAGTTCGACCAGATGATTCA 382 397 123 Antisense
GGTACAAGATCTTCCACTGATGATG 624 503 151 Antisense
GTACATTCGATGTGCTCTCAGCAAA 582 459 175 Antisense
AAATGTCAAAGTCCCAATTGCACCA 45 121 198 Antisense
CACCAACATCTGCTCAAATGACATT 353 203 218 Antisense
ATAAGCTGGCTCTGAAAGTTCCGAA 409 25 275 Antisense
TACGACAGTCGATGTGGCGGCTCAA 347 649 303 Antisense
GGCGGCTCAAAATCTTGCCGAGCAA 457 541 318 Antisense
Sequence Source Affymetrix Proprietary Database