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[181981_at]

Description B0212.3 /REP_DB=WormBase Gene ID /WP=CE16769 /TR=O45063 /GB=AAC02565.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 54      4grp_total > 23      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 22.9
(-0.6)
A 		39.5
(3)
A 		11.5
(-3)
A 		36.1
(2.3)
A 		28
B 31.1
(3)
A 		5.5
(-2.3)
A 		15.2
(-0.3)
A 		2.2
(-3)
A 		29
C 38.2
(2.4)
A 		9.9
(-3)
A 		27.2
(0.3)
A 		41.1
(3)
A 		31
avr 30.7
(3) 		18.3
(-2.9) 		18
(-3) 		26.5
(1) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 12
(-2.1)
A 		59.1
(2.2)
A 		67.9
(3)
P 		1.7
(-3)
A 		50.6
(1.4)
A 		52.2
(1.6)
P 		66
B 21.9
(-2.7)
A 		24.7
(-2.2)
P 		50.3
(3)
A 		20.6
(-3)
A 		40.3
(1)
P 		49.2
(2.8)
P 		30
C 41.9
(1.2)
A 		49.1
(2.5)
P 		47.6
(2.2)
M 		52.2
(3)
A 		20.5
(-2.5)
A 		17.5
(-3)
A 		35
avr 25.3
(-2.9) 		44.3
(0.8) 		55.3
(3) 		24.8
(-3) 		37.1
(-0.6) 		39.6
(-0.1) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 8.3
(2.7)
A 		8.6
(3)
A 		3
(-3)
A 		5.3
(-0.5)
A 		6
B 9.9
(3)
A 		1.4
(-2.4)
A 		3.5
(-1.1)
A 		0.5
(-3)
A 		9
C 12.7
(3)
A 		2.9
(-3)
A 		9.5
(1)
A 		6.9
(-0.6)
A 		10
avr 10.3
(3) 		4.3
(-2.9) 		5.3
(-1.9) 		4.2
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 2.2
(-2.5)
A 		21
(3)
A 		14.8
(1.2)
P 		0.4
(-3)
A 		15.3
(1.3)
A 		12.7
(0.6)
P 		21
B 7.6
(-2)
A 		8.2
(-1.7)
P 		12.2
(0.1)
A 		5.5
(-3)
A 		16.4
(2.1)
P 		18.3
(3)
P 		13
C 15.5
(3)
A 		15
(2.7)
P 		12.1
(0.9)
M 		10.5
(-0.1)
A 		6.6
(-2.6)
A 		5.9
(-3)
A 		10
avr 8.4
(-1.1) 		14.7
(3) 		13
(1.9) 		5.5
(-3) 		12.8
(1.8) 		12.3
(1.4) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181981_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11045
Sequence Type Exemplar sequence
Representative Public ID B0212.3 NCBI
Target Description B0212.3 /REP_DB=WormBase Gene ID /WP=CE16769 /TR=O45063 /GB=AAC02565.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067968(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067968 NCBI Caenorhabditis elegans B0212.3 (B0212.3) mRNA, complete cds. 11/11 A 
SNAP00000040977 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3540173:3543169:1 10/11 None
GENEFINDER00000040985 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3540184:3543527:1 11/11 None
B0212.3 ENSEMBL cdna:known chromosome:CEL140:IV:3540184:3543532:1 gene:B0212.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3540189-3543533(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0212.3
Entrez Gene 177115 Entrez gene
SwissProt O45063 EMBL-EBI
WormBase CE16769 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC02565 Hypothetical protein B0212.3 [Caenorhabditis elegans] ref|NP_500369.1| B0212.3 [Caenorhabditis elegans] 0.0
blast AAC68984 Hypothetical protein M57.1 [Caenorhabditis elegans] ref|NP_500368.1| M57.1 [Caenorhabditis elegans] 0.0
blast AAC16990 Hypothetical protein B0205.1 [Caenorhabditis elegans] ref|NP_492805.2| B0205.1 [Caenorhabditis elegans] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR006570 EMBL-EBI SPK 7.5E-60
Pfam IPR006570 EMBL-EBI SPK 1.3E-60
Pfam IPR006570 EMBL-EBI SPK 3.0E-63
Pfam IPR006570 EMBL-EBI SPK 7.5E-60
Pfam IPR006570 EMBL-EBI SPK 1.3E-60
Pfam IPR006570 EMBL-EBI SPK 7.1E-17
Pfam IPR006570 EMBL-EBI SPK 1.8E-10
Pfam IPR006570 EMBL-EBI SPK 2.4E-39
Pfam IPR006570 EMBL-EBI SPK 1.3E-60
Pfam IPR006570 EMBL-EBI SPK 3.0E-63
Sequence
Target Sequence 
>C. ELEGANS:181981_AT
gtgatctaacgacggacggtccatcatcgaatgcacatgatctaacaatgtggcctaacg
atgacccgaagcaagagcctgtctttgagaatatcttcatacaaaatcaatactttcctc
cagaaattgaagaggttccaaatcaaggagtttaccatgttgagccagaaacaaagcttt
cttatattaacactaatccaggatcaagtgtcactgaatctggcgggattaagcttcata
aattcttgaatttgttagaactcattcttacttctctcgaatcagatactctagatgatt
tgttaaacgatattgaagggattcgtgttcgaataaatgatgagattcttccaccagagc
tagtgatcactgccttgcagacgttcttattgggaatcactcgcaagtctcgatccgcag
cgtcagcagaaaacaaatcatcacacgagtgtctgagaatcttcaaatattcattgcctt
ggctcagaagccctttgctttgggagctacaacagagggttgaggaagaaattgaaagtc
acgttatcggagggaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGATCTAACGACGGACGGTCCATC 307 483 1412 Antisense
CATCGAATGCACATGATCTAACAAT 42 211 1436 Antisense
ACAATGTGGCCTAACGATGACCCGA 535 113 1456 Antisense
GATGACCCGAAGCAAGAGCCTGTCT 573 411 1471 Antisense
GAGCCTGTCTTTGAGAATATCTTCA 563 385 1486 Antisense
ACAAAATCAATACTTTCCTCCAGAA 165 113 1512 Antisense
CAAGGAGTTTACCATGTTGAGCCAG 635 181 1555 Antisense
AAGCTTTCTTATATTAACACTAATC 468 147 1585 Antisense
ATCAAGTGTCACTGAATCTGGCGGG 372 25 1614 Antisense
ATCTGGCGGGATTAAGCTTCATAAA 391 33 1629 Antisense
GAAAGTCACGTTATCGGAGGGAAGA 328 357 1945 Antisense
Sequence Source Affymetrix Proprietary Database