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[181977_s_at]

Description F42C5.9 /REP_DB=WormBase Gene ID /WP=CE04562 /TR=Q20329 /GB=AAA81487.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 02      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 75      6grp_total > 07      4grp_total > 47      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 400
1 2 3 4 range
A 883.1
(3)
P 		685.5
(-0.6)
P 		550.5
(-3)
P 		693.7
(-0.4)
P 		333
B 926.8
(3)
P 		565.8
(-2.6)
P 		546
(-2.9)
P 		536.9
(-3)
P 		390
C 936.7
(3)
P 		553.3
(-3)
P 		656.9
(-1.4)
P 		625.5
(-1.9)
P 		383
avr 915.5
(3) 		601.5
(-2.7) 		584.5
(-3) 		618.7
(-2.4) 		331

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 459
1 2 3 4 5 6 range
A 1067.4
(3)
P 		943.8
(1.1)
P 		848.3
(-0.4)
P 		947.4
(1.1)
P 		684.4
(-3)
P 		893
(0.3)
P 		383
B 975.1
(2.7)
P 		807.8
(-0.9)
P 		865.6
(0.3)
P 		814.6
(-0.8)
P 		711.6
(-3)
P 		991.4
(3)
P 		280
C 1135.4
(3)
P 		935.9
(0.4)
P 		1098.6
(2.5)
P 		923
(0.2)
P 		676.7
(-3)
P 		753.6
(-2)
P 		459
avr 1059.3
(3) 		895.8
(0.3) 		937.5
(1) 		895
(0.3) 		690.9
(-3) 		879.3
(0.1) 		368
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 321.5
(3)
P 		148.8
(-1.7)
P 		143.9
(-1.8)
P 		101.5
(-3)
P 		220
B 295.5
(3)
P 		139.7
(-2.2)
P 		126.5
(-2.6)
P 		114.4
(-3)
P 		181
C 310.1
(3)
P 		159.1
(-1.4)
P 		228.9
(0.6)
P 		105.5
(-3)
P 		205
avr 309
(3) 		149.2
(-1.7) 		166.4
(-1.2) 		107.1
(-3) 		202

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 236
1 2 3 4 5 6 range
A 196.8
(-2.5)
P 		335.7
(3)
P 		184.8
(-3)
P 		240.3
(-0.8)
P 		206.5
(-2.1)
P 		217.4
(-1.7)
P 		151
B 339.3
(1.9)
P 		268.9
(-0.8)
P 		210.2
(-3)
P 		215.8
(-2.8)
P 		289.1
(-0)
P 		369.7
(3)
P 		160
C 420.8
(3)
P 		285.6
(-0.4)
P 		279.6
(-0.6)
P 		185.6
(-3)
P 		219
(-2.1)
P 		252.3
(-1.3)
P 		235
avr 319
(3) 		296.7
(1.7) 		224.9
(-2.4) 		213.9
(-3) 		238.2
(-1.6) 		279.8
(0.8) 		105
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181977_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9151
Sequence Type Exemplar sequence
Representative Public ID F42C5.9 NCBI
Target Description F42C5.9 /REP_DB=WormBase Gene ID /WP=CE04562 /TR=Q20329 /GB=AAA81487.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068768(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068768 NCBI Caenorhabditis elegans F42C5.9 (F42C5.9) mRNA, complete cds. 11/11 None
SNAP00000034683 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7314677:7317078:-1 11/11 None
GENEFINDER00000034685 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7314677:7317078:-1 11/11 None
F42C5.9.1 ENSEMBL cdna:known chromosome:CEL140:IV:7314358:7317087:-1 gene:F42C5.9 11/11 None
F42C5.9.2 ENSEMBL cdna:known chromosome:CEL140:IV:7314365:7317346:-1 gene:F42C5.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7314682-7317084(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F42C5.9
Entrez Gene 177504 Entrez gene
SwissProt Q20329 EMBL-EBI
WormBase CE04562 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
15629 actin cytoskeleton inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5200 structural constituent of cytoskeleton inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81487 Hypothetical protein F42C5.9 [Caenorhabditis elegans] ref|NP_501169.1| F42C5.9 [Caenorhabditis elegans] 0.0
blast CAE61856 Hypothetical protein CBG05834 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004000 EMBL-EBI Actin/actin-like 5.0E-41
Sequence
Target Sequence 
>C. ELEGANS:181977_S_AT
aatcggccatttgttcaattccatatggatcacagcaaatgggagattcgttaagagcat
cattaagtgctcataacaaaggattatacgcttttcaaagtcctgtggagaagcttattt
taagatttgcactggaacagactacctacgtaccagaggattatcaaaaagaagaaaaat
cagaaaataaagaaactagaatctcattagatgctttcacaccatcacctggaatgcaga
cccgattcgatattgataactccagatttttatgtacggaaggactattcaagccaaaga
aatggggtcttgacacgaaaggtctccaccaattgatccacgaggcaattcttcagtgtc
caattgatagtcgtcgaacactgtatagaaatatttatttggctggaggagcatctctta
tgccaggaattgctgaaaaactcgaacaagaattatctacagtagttccaaacacaattc
acactcaagttaacatcagtccatggcggtataacgcagcatatcttggagcccaaatcg
tcgcatcagcctccacatttgaagactcctgcatttctcttcaacagcttccaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCGGCCATTTGTTCAATTCCATA 154 169 1043 Antisense
CACTGGAACAGACTACCTACGTACC 263 199 1172 Antisense
TTAGATGCTTTCACACCATCACCTG 639 681 1249 Antisense
CCATCACCTGGAATGCAGACCCGAT 444 271 1264 Antisense
GGTCTTGACACGAAAGGTCTCCACC 336 501 1348 Antisense
TCCACGAGGCAATTCTTCAGTGTCC 421 589 1379 Antisense
GGCTGGAGGAGCATCTCTTATGCCA 188 541 1443 Antisense
TATAACGCAGCATATCTTGGAGCCC 240 659 1552 Antisense
GCATCAGCCTCCACATTTGAAGACT 275 309 1585 Antisense
ATTTGAAGACTCCTGCATTTCTCTT 689 17 1599 Antisense
CATTTCTCTTCAACAGCTTCCAAAG 454 215 1614 Antisense
Sequence Source Affymetrix Proprietary Database