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[181974_s_at]

Description F27C1.4 /REP_DB=WormBase Gene ID /WP=CE09717 /TR=P91281 /GB=AAB37652.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 08      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 22.4
(-3)
A 		38.5
(-1.5)
A 		54.2
(-0)
A 		86.3
(3)
M 		64
B 7.7
(-3)
A 		10.4
(-2.8)
A 		43.5
(-0.4)
A 		88.8
(3)
P 		81
C 6.6
(-2.8)
A 		3.9
(-3)
A 		25.4
(-1.2)
A 		76.6
(3)
P 		73
avr 12.2
(-3) 		17.6
(-2.5) 		41
(-0.6) 		83.9
(3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 90.9
(3)
P 		30.1
(-3)
A 		49.9
(-1)
A 		37.8
(-2.2)
A 		82.6
(2.2)
P 		85.8
(2.5)
P 		61
B 52.5
(-0.8)
P 		33.3
(-3)
A 		74.4
(1.7)
P 		70.8
(1.3)
P 		82.2
(2.6)
P 		85.4
(3)
P 		52
C 18.6
(-3)
A 		40
(-1.5)
A 		43.1
(-1.3)
P 		60.8
(0)
A 		82.8
(1.6)
P 		102.9
(3)
P 		84
avr 54
(-0.9) 		34.5
(-3) 		55.8
(-0.8) 		56.5
(-0.7) 		82.5
(2.1) 		91.4
(3) 		57
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 8.1
(-3)
A 		8.4
(-2.7)
A 		14.2
(3)
A 		12.6
(1.4)
M 		6
B 2.5
(-3)
A 		2.6
(-3)
A 		10.1
(-0.2)
A 		18.9
(3)
P 		16
C 2.2
(-2.4)
A 		1.1
(-3)
A 		8.9
(1)
A 		12.9
(3)
P 		12
avr 4.3
(-2.8) 		4
(-3) 		11.1
(0.9) 		14.8
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 16.8
(-0.2)
P 		10.7
(-2.6)
A 		10.9
(-2.5)
A 		9.6
(-3)
A 		24.9
(3)
P 		20.9
(1.4)
P 		15
B 18.3
(-1.1)
P 		11.1
(-3)
A 		18.1
(-1.1)
P 		18.8
(-0.9)
P 		33.4
(3)
P 		31.9
(2.6)
P 		22
C 6.9
(-3)
A 		12.2
(-1.8)
A 		11
(-2.1)
P 		12.2
(-1.8)
A 		26.8
(1.3)
P 		34.5
(3)
P 		28
avr 14
(-2.1) 		11.3
(-3) 		13.3
(-2.3) 		13.5
(-2.3) 		28.4
(2.8) 		29.1
(3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181974_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1211
Sequence Type Exemplar sequence
Representative Public ID F27C1.4 NCBI
Target Description F27C1.4 /REP_DB=WormBase Gene ID /WP=CE09717 /TR=P91281 /GB=AAB37652.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059190(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059190 NCBI Caenorhabditis elegans F27C1.4 (F27C1.4) mRNA, complete cds. 11/11 A 
SNAP00000014499 ENSEMBL cdna:SNAP chromosome:CEL140:I:5422789:5423208:1 11/11 None
GENEFINDER00000014523 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5422822:5424891:1 9/11 None
F27C1.4 ENSEMBL cdna:known chromosome:CEL140:I:5422789:5423208:1 gene:F27C1.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5422788-5423208(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F27C1.4
Entrez Gene 172193 Entrez gene
SwissProt P91281 EMBL-EBI
WormBase CE09717 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37652 Hypothetical protein F27C1.4 [Caenorhabditis elegans] ref|NP_491591.1| F27C1.4 [Caenorhabditis elegans] 1.0E-74
blast AAB37652 Hypothetical protein F27C1.4 [Caenorhabditis elegans] ref|NP_491591.1| F27C1.4 [Caenorhabditis elegans] 5.0E-63
blast CAE66924 Hypothetical protein CBG12312 [Caenorhabditis briggsae] 2.0E-51
blast CAE66924 Hypothetical protein CBG12312 [Caenorhabditis briggsae] 6.0E-41
blast CAE67188 Hypothetical protein CBG12624 [Caenorhabditis briggsae] 3.0E-10
Sequence
Target Sequence 
>C. ELEGANS:181974_S_AT
ccatcgtcaatggactctacaccttcatgtccacaaccgtcgtcccagcagtcacagaac
aggcagcgaaccttgttggaggagcttctgcacttgccgaaggagcaacacaggctctaa
cacaaatcagtcagactgctctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCATCGTCAATGGACTCTACACCTT 376 271 20 Antisense
TGGACTCTACACCTTCATGTCCACA 698 547 30 Antisense
TCGTCCCAGCAGTCACAGAACAGGC 382 605 59 Antisense
GCAGTCACAGAACAGGCAGCGAACC 647 309 67 Antisense
GAACAGGCAGCGAACCTTGTTGGAG 446 351 76 Antisense
GGCAGCGAACCTTGTTGGAGGAGCT 611 533 81 Antisense
CCTTGTTGGAGGAGCTTCTGCACTT 355 267 90 Antisense
TCTGCACTTGCCGAAGGAGCAACAC 385 607 106 Antisense
GAAGGAGCAACACAGGCTCTAACAC 203 335 118 Antisense
ACACAGGCTCTAACACAAATCAGTC 693 109 127 Antisense
AACACAAATCAGTCAGACTGCTCTC 200 135 138 Antisense
Sequence Source Affymetrix Proprietary Database