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[181972_at]

Description W05E7.1 /REP_DB=WormBase Gene ID /WP=CE17288 /TR=Q9TYW7 /GB=AAC68996.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 74      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 39      6grp_total > 64      4grp_total > 10      6grp_cgc > 56      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13289
1 2 3 4 range
A 2138.5
(-3)
P 		4356.3
(-2)
P 		3379.7
(-2.4)
P 		15377.8
(3)
P 		13239
B 2088.5
(-3)
P 		3859.4
(-2.1)
P 		2124.2
(-3)
P 		13943.6
(3)
P 		11855
C 2302.9
(-3)
P 		2686.3
(-2.8)
P 		2656.7
(-2.8)
P 		13062.2
(3)
P 		10759
avr 2176.6
(-3) 		3634
(-2.3) 		2720.2
(-2.7) 		14127.9
(3) 		11951

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9378
1 2 3 4 5 6 range
A 2919.5
(-3)
P 		5657.5
(-0.8)
P 		9427.6
(2.2)
P 		7540.6
(0.7)
P 		4946.9
(-1.4)
P 		10423.7
(3)
P 		7504
B 2530.6
(-3)
P 		4528.6
(-1.6)
P 		10830.6
(3)
P 		6478.5
(-0.1)
P 		5050.8
(-1.2)
P 		8077.6
(1)
P 		8300
C 2936.9
(-3)
P 		4383.1
(-2)
P 		11908.4
(3)
P 		6710.1
(-0.5)
P 		6329.7
(-0.7)
P 		8603.2
(0.8)
P 		8972
avr 2795.7
(-3) 		4856.4
(-1.4) 		10722.2
(3) 		6909.7
(0.1) 		5442.5
(-1) 		9034.8
(1.7) 		7927
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2479
1 2 3 4 range
A 778.6
(-3)
P 		945.6
(-2.3)
P 		883.5
(-2.6)
P 		2251
(3)
P 		1472
B 666
(-2.6)
P 		953.2
(-1.9)
P 		492.2
(-3)
P 		2971.4
(3)
P 		2479
C 762.3
(-3)
P 		772.3
(-3)
P 		925.9
(-2.3)
P 		2202.5
(3)
P 		1440
avr 735.6
(-3) 		890.4
(-2.5) 		767.2
(-2.9) 		2475
(3) 		1739

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2493
1 2 3 4 5 6 range
A 538.2
(-3)
P 		2012.1
(1.4)
P 		2053.1
(1.5)
P 		1912.5
(1.1)
P 		1492.8
(-0.1)
P 		2537.9
(3)
P 		2000
B 880.5
(-3)
P 		1507.4
(-1.2)
P 		2630
(1.9)
P 		1715.9
(-0.6)
P 		2052.1
(0.3)
P 		3012.1
(3)
P 		2132
C 1088.6
(-3)
P 		1337.4
(-2.2)
P 		3030.9
(3)
P 		1349.4
(-2.2)
P 		2048.2
(-0)
P 		2880
(2.5)
P 		1942
avr 835.8
(-3) 		1619
(-0.6) 		2571.3
(2.3) 		1659.3
(-0.5) 		1864.4
(0.1) 		2810
(3) 		1974
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181972_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11030
Sequence Type Exemplar sequence
Representative Public ID W05E7.1 NCBI
Target Description W05E7.1 /REP_DB=WormBase Gene ID /WP=CE17288 /TR=Q9TYW7 /GB=AAC68996.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067945(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067945 NCBI Caenorhabditis elegans GRounDhog (hedgehog-like family) family member (grd-3) (grd-3) mRNA, complete cds. 10/11 None
SNAP00000010657 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3439492:3440329:1 9/11 None
W05E7.1 ENSEMBL cdna:known chromosome:CEL140:IV:3439654:3440438:1 gene:W05E7.1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000010658 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3438364-3440335(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grd-3
Ensembl W05E7.1
Entrez Gene 177109 Entrez gene
SwissProt Q9TYW7 EMBL-EBI
WormBase CE34103 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
42828 response to pathogen inferred from direct assay QuickGO AmiGO
7154 cell communication inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5102 receptor binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAP40517 Groundhog (hedgehog-like family) protein 3 [Caenorhabditis elegans] ref|NP_500346.2| GRounDhog (hedgehog-like family) family member (grd-3) [Caenorhabditis elegans] 3.0E-87
blast AAP40517 Groundhog (hedgehog-like family) protein 3 [Caenorhabditis elegans] ref|NP_500346.2| GRounDhog (hedgehog-like family) family member (grd-3) [Caenorhabditis elegans] 3.0E-80
blast CAE74910 Hypothetical protein CBG22788 [Caenorhabditis briggsae] 3.0E-66
blast CAE74910 Hypothetical protein CBG22788 [Caenorhabditis briggsae] 4.0E-63
blast AAB52858 Groundhog (hedgehog-like family) protein 4 [Caenorhabditis elegans] ref|NP_509442.1| GRounDhog (hedgehog-like family) family member (grd-4) [Caenorhabditis elegans] 5.0E-56
blast AAB52858 Groundhog (hedgehog-like family) protein 4 [Caenorhabditis elegans] ref|NP_509442.1| GRounDhog (hedgehog-like family) family member (grd-4) [Caenorhabditis elegans] 5.0E-53
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 5.2E-24
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 5.2E-24
Sequence
Target Sequence 
>C. ELEGANS:181972_AT
cacgcaacgtcatcgaaggtctcactataaaagggacggcgccggcgctggaaggatcag
tgttcagctcggcagcgatgaagtttctactccttcttggcgcggttatagccgttcgag
cccaatccgacacatgcccatcactcaaggatcgtcagcttgccaagcctggccagctgt
tctgctgtgatagcaccatcaaaaccgtcgtggagaccggaatgcagaccctggacctct
tcggagctggtggaccacaaacacttggaccaatcgttcaggcgctaagcaccttcgttc
agcaacacttcaaaactgcctatgagattgttatggctccaaaggactttgtgctcaaca
cacactacaatggcaccagtctctgcaagttccagtccaacagttacaccttggccatct
acgagaccccagctaaatacgatatcaacagctccagagaaaagtacttcaacaagttcg
ctctcaacgacaagctccgtctgccatcagtttccaagcacctcaagcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACGCAACGTCATCGAAGGTCTCAC 421 199 470 Antisense
TCAGTGTTCAGCTCGGCAGCGATGA 265 623 526 Antisense
GATGAAGTTTCTACTCCTTCTTGGC 69 413 546 Antisense
TTGGCGCGGTTATAGCCGTTCGAGC 469 605 566 Antisense
TGGACCAATCGTTCAGGCGCTAAGC 422 547 735 Antisense
GCTCAACACACACTACAATGGCACC 337 301 822 Antisense
CAATGGCACCAGTCTCTGCAAGTTC 470 183 837 Antisense
AGTTCCAGTCCAACAGTTACACCTT 709 63 857 Antisense
CATCTACGAGACCCCAGCTAAATAC 555 209 885 Antisense
CAAGTTCGCTCTCAACGACAAGCTC 207 185 942 Antisense
ATCAGTTTCCAAGCACCTCAAGCAA 444 29 975 Antisense
Sequence Source Affymetrix Proprietary Database