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[181968_s_at]

Description Y48C3A.7 /REP_DB=WormBase Gene ID /WP=CE28132 /TR=Q9NAG4 /GB=CAB55106.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 20      6grp_total > 69      4grp_total > 12      6grp_cgc > 39      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 182
1 2 3 4 range
A 151.6
(1.2)
P 		121.5
(-0.4)
P 		70.5
(-3)
P 		186.9
(3)
A 		116
B 104.8
(0.5)
P 		73.2
(-2.2)
A 		64.5
(-3)
A 		133.7
(3)
P 		69
C 122.2
(-2.3)
P 		109.6
(-2.8)
A 		105.8
(-3)
A 		246.3
(3)
P 		141
avr 126.2
(-0.5) 		101.4
(-1.8) 		80.3
(-3) 		189
(3) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 71.6
(0.5)
A 		25.1
(-3)
A 		55.7
(-0.7)
A 		53.6
(-0.8)
A 		72.4
(0.6)
A 		103.7
(3)
P 		79
B 43.4
(-0.9)
A 		33.9
(-3)
A 		55.3
(1.7)
A 		55.1
(1.6)
A 		57.9
(2.3)
A 		61.3
(3)
P 		27
C 63.9
(2)
A 		51.9
(0.4)
A 		45.9
(-0.5)
A 		27.2
(-3)
A 		31.1
(-2.5)
A 		71.2
(3)
P 		44
avr 59.6
(0.3) 		37
(-3) 		52.3
(-0.8) 		45.3
(-1.8) 		53.8
(-0.6) 		78.7
(3) 		42
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 55.2
(3)
P 		26.4
(-1.7)
P 		18.4
(-3)
P 		27.4
(-1.5)
A 		37
B 33.4
(3)
P 		18.1
(-2)
A 		14.9
(-3)
A 		28.5
(1.4)
P 		19
C 40.4
(2.3)
P 		31.5
(-3)
A 		36.9
(0.2)
A 		41.5
(3)
P 		10
avr 43
(3) 		25.3
(-2.4) 		23.4
(-3) 		32.5
(-0.2) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 13.2
(-1.4)
A 		8.9
(-3)
A 		12.1
(-1.8)
A 		13.6
(-1.3)
A 		21.9
(1.8)
A 		25.2
(3)
P 		16
B 15.1
(-1.1)
A 		11.3
(-3)
A 		13.4
(-2)
A 		14.6
(-1.4)
A 		23.5
(3)
A 		22.9
(2.7)
P 		12
C 23.7
(3)
A 		15.8
(0.4)
A 		11.7
(-1)
A 		5.5
(-3)
A 		10.1
(-1.5)
A 		23.8
(3)
P 		18
avr 17.3
(-0.1) 		12
(-2.6) 		12.4
(-2.4) 		11.2
(-3) 		18.5
(0.4) 		24
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181968_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3560
Sequence Type Exemplar sequence
Representative Public ID Y48C3A.7 NCBI
Target Description Y48C3A.7 /REP_DB=WormBase Gene ID /WP=CE28132 /TR=Q9NAG4 /GB=CAB55106.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064413(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064413 NCBI Caenorhabditis elegans Member of AAA family binding CED-4 family member (mac-1) (mac-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000006663 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:13320305:13320805:-1 11/11 None
Y48C3A.7 ENSEMBL cdna:known chromosome:CEL140:II:13311381:13320807:-1 gene:Y48C3A.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13320301-13320802(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mac-1
Ensembl Y48C3A.7
Entrez Gene 174974 Entrez gene
SwissProt Q9NAG4 EMBL-EBI
WormBase CE28132 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:259176_AT AAA-type ATPase family protein at
CANINE_2:CFAAFFX.25070.1.S1_S_AT similar to Nuclear valosin-containing protein-like (Nuclear VCP-like protein) (NVLp) cfa
CHICKEN:GGA.2302.1.S1_AT similar to Nuclear valosin-containing protein-like (Nuclear VCP-like protein) (NVLp) gga
CHICKEN:GGA.2302.2.S1_AT similar to Nuclear valosin-containing protein-like (Nuclear VCP-like protein) (NVLp) gga
CHICKEN:GGAAFFX.11968.1.S1_S_AT similar to Nuclear valosin-containing protein-like (Nuclear VCP-like protein) (NVLp) gga
CHICKEN:GGAAFFX.22884.1.S1_S_AT similar to Nuclear valosin-containing protein-like (Nuclear VCP-like protein) (NVLp) gga
CHICKEN:GGAAFFX.5869.1.S1_S_AT similar to Nuclear valosin-containing protein-like (Nuclear VCP-like protein) (NVLp) gga
HG-U133_PLUS_2:207877_S_AT nuclear VCP-like hs
HG-U133A:207877_S_AT nuclear VCP-like hs
HG-FOCUS:207877_S_AT nuclear VCP-like hs
HG-U133A_2:207877_S_AT nuclear VCP-like hs
HU35KSUBB:RC_AA128935_AT nuclear VCP-like hs
U133_X3P:G4505482_3P_A_AT nuclear VCP-like hs
HG-U95AV2:40285_AT nuclear VCP-like hs
U133_X3P:HS2.407575.1.S1_3P_AT Nuclear VCP-like hs
HG-U133_PLUS_2:1561530_AT Nuclear VCP-like hs
HG-U95C:63165_R_AT Nuclear VCP-like hs
HU35KSUBD:RC_AA206444_AT Nuclear VCP-like hs
MG-U74AV2:95357_AT nuclear VCP-like mm
MU11KSUBB:MSA.35598.0_S_AT nuclear VCP-like mm
MU11KSUBB:W44048_AT nuclear VCP-like mm
MG-U74AV2:101366_F_AT nuclear VCP-like mm
MG-U74BV2:111130_AT nuclear VCP-like mm
MG-U74CV2:170638_AT nuclear VCP-like mm
MOE430A:1453018_AT nuclear VCP-like mm
MOUSE430A_2:1453018_AT nuclear VCP-like mm
MOUSE430_2:1453018_AT nuclear VCP-like mm
MOE430A:1453019_AT nuclear VCP-like mm
MOUSE430A_2:1453019_AT nuclear VCP-like mm
MOUSE430_2:1453019_AT nuclear VCP-like mm
MOUSE430A_2:1448047_AT Nuclear VCP-like, mRNA (cDNA clone MGC:25549 IMAGE:3964470) mm
MOE430A:1448047_AT Nuclear VCP-like, mRNA (cDNA clone MGC:25549 IMAGE:3964470) mm
MOUSE430_2:1448047_AT Nuclear VCP-like, mRNA (cDNA clone MGC:25549 IMAGE:3964470) mm
MG-U74AV2:96565_AT Nuclear VCP-like, mRNA (cDNA clone MGC:25549 IMAGE:3964470) mm
MU11KSUBA:AA168433_S_AT Nuclear VCP-like, mRNA (cDNA clone MGC:25549 IMAGE:3964470) mm
MU19KSUBC:TC37466_AT Nuclear VCP-like, mRNA (cDNA clone MGC:25549 IMAGE:3964470) mm
RG-U34A:RC_AA893753_AT nuclear VCP-like (predicted) rn
RAE230A:1389314_AT nuclear VCP-like (predicted) rn
RAT230_2:1389314_AT nuclear VCP-like (predicted) rn
YEAST_2:1778653_AT Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
166 nucleotide binding inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB55106 Hypothetical protein Y48C3A.7 [Caenorhabditis elegans] ref|NP_496814.1| Member of AAA family binding CED-4 family member (mac-1) [Caenorhabditis elegans] 0.0
blast AAF05624 cell survival CED-4-interacting protein MAC-1 [Caenorhabditis elegans] 0.0
blast T31590 hypothetical protein Y48C3A.h - Caenorhabditis elegans 0.0
blast CAB55106 Hypothetical protein Y48C3A.7 [Caenorhabditis elegans] ref|NP_496814.1| Member of AAA family binding CED-4 family member (mac-1) [Caenorhabditis elegans] 3.0E-20
blast AAF05624 cell survival CED-4-interacting protein MAC-1 [Caenorhabditis elegans] 3.0E-20
blast CAE73363 Hypothetical protein CBG20797 [Caenorhabditis briggsae] 1.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR003959 EMBL-EBI AAA ATPase, central region 2.0E-65
Pfam IPR003959 EMBL-EBI AAA ATPase, central region 5.2E-85
Sequence
Target Sequence 
>C. ELEGANS:181968_S_AT
atgcctggcggaatgggttttcctagtgatccggcacttttgccacgtgtacaggctcac
attcgaaagtttcccggtacaaaatactttaagccggagcttgtcgcctacgatttgcag
caagagcatcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCCTGGCGGAATGGGTTTTCCTA 218 41 13 Antisense
AATGGGTTTTCCTAGTGATCCGGCA 305 163 24 Antisense
TTCCTAGTGATCCGGCACTTTTGCC 177 699 32 Antisense
CACGTGTACAGGCTCACATTCGAAA 112 199 56 Antisense
ACAGGCTCACATTCGAAAGTTTCCC 497 105 63 Antisense
TCACATTCGAAAGTTTCCCGGTACA 213 591 69 Antisense
GTACAAAATACTTTAAGCCGGAGCT 326 457 89 Antisense
ACTTTAAGCCGGAGCTTGTCGCCTA 333 101 98 Antisense
GCTTGTCGCCTACGATTTGCAGCAA 444 307 111 Antisense
GTCGCCTACGATTTGCAGCAAGAGC 130 475 115 Antisense
CTACGATTTGCAGCAAGAGCATCCA 360 225 120 Antisense
Sequence Source Affymetrix Proprietary Database