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[181961_at]

Description T22H9.4 /REP_DB=WormBase Gene ID /WP=CE20102 /TR=Q9TXN8 /GB=AAC69225.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 39      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 148
1 2 3 4 range
A 87.1
(3)
P 		50
(-3)
A 		49.9
(-3)
A 		79.3
(1.7)
A 		37
B 149.1
(3)
P 		47.8
(-3)
A 		74.1
(-1.4)
A 		67.2
(-1.9)
P 		101
C 163.5
(3)
P 		15.3
(-3)
A 		28.2
(-2.5)
A 		82.8
(-0.3)
A 		148
avr 133.2
(3) 		37.7
(-3) 		50.7
(-2.2) 		76.4
(-0.6) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 56.8
(0.7)
A 		84
(3)
A 		64
(1.3)
P 		43.3
(-0.4)
A 		12.3
(-3)
A 		55.7
(0.6)
A 		72
B 100.5
(3)
P 		41.2
(-1.3)
A 		30.8
(-2.1)
A 		70.1
(0.8)
P 		61.1
(0.1)
A 		17.7
(-3)
A 		83
C 60.6
(-0.9)
A 		29.6
(-2.9)
A 		30.8
(-2.8)
A 		120.3
(3)
P 		28.3
(-3)
A 		70.2
(-0.3)
A 		92
avr 72.6
(2.3) 		51.6
(-0.6) 		41.9
(-1.9) 		77.9
(3) 		33.9
(-3) 		47.9
(-1.1) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 31.7
(3)
P 		10.9
(-3)
A 		13
(-2.4)
A 		11.6
(-2.8)
A 		21
B 47.6
(3)
P 		11.8
(-3)
A 		17.2
(-2.1)
A 		14.3
(-2.6)
P 		36
C 54.1
(3)
P 		4.4
(-3)
A 		9.8
(-2.3)
A 		14
(-1.8)
A 		50
avr 44.5
(3) 		9
(-3) 		13.3
(-2.3) 		13.3
(-2.3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 10.5
(-1.4)
A 		29.9
(3)
A 		13.9
(-0.7)
P 		11
(-1.3)
A 		3.7
(-3)
A 		13.6
(-0.7)
A 		26
B 35
(3)
P 		13.7
(-1.5)
A 		7.5
(-2.8)
A 		18.6
(-0.5)
P 		24.8
(0.8)
A 		6.6
(-3)
A 		28
C 22.4
(2.3)
A 		9
(-2.6)
A 		7.8
(-3)
A 		24.2
(3)
P 		9.1
(-2.5)
A 		23.5
(2.7)
A 		16
avr 22.6
(3) 		17.5
(0.6) 		9.7
(-3) 		17.9
(0.8) 		12.5
(-1.7) 		14.6
(-0.7) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181961_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15536
Sequence Type Exemplar sequence
Representative Public ID T22H9.4 NCBI
Target Description T22H9.4 /REP_DB=WormBase Gene ID /WP=CE20102 /TR=Q9TXN8 /GB=AAC69225.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070775(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070775 NCBI Caenorhabditis elegans T22H9.4 (T22H9.4) mRNA, complete cds. 11/11 None
SNAP00000007599 ENSEMBL cdna:SNAP chromosome:CEL140:V:334682:336267:-1 11/11 None
GENEFINDER00000007607 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:334682:336267:-1 11/11 None
T22H9.4 ENSEMBL cdna:known chromosome:CEL140:V:334475:336690:-1 gene:T22H9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:334681-336717(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T22H9.4
Entrez Gene 188771 Entrez gene
SwissProt Q9TXN8 EMBL-EBI
WormBase CE33328 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
7548 sex differentiation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC69225 Hypothetical protein T22H9.4 [Caenorhabditis elegans] ref|NP_503176.2| T22H9.4 [Caenorhabditis elegans] 1.0E-149
blast CAE58275 Hypothetical protein CBG01382 [Caenorhabditis briggsae] 1.0E-106
Protein Domains
Database ID Description E-Value
Pfam IPR001275 EMBL-EBI DM DNA-binding 2.1E-7
Pfam IPR001275 EMBL-EBI DM DNA-binding 1.7E-13
Sequence
Target Sequence 
>C. ELEGANS:181961_AT
gtgtaacttgattgattcccgtcgtgccctggaccggcatattaagaagcgaaaaatgag
cataaagggcaacacggttgaagctatcgcaccgaaaagtcccaaaaaagagggctccac
cacggcttcatcatcgtcactctcatcttcttcgggctgcaattcggacgagtcggcttc
atcctcggattgctcgctatctggaatgctatcagagaagttgaacatggctccgcaggt
tcagatgaaattcgacttcagtgccgggggatttatgattcctgcagcgacttcggcaaa
tgcaagcactagtccggctggttctccatttgagacaacatctccaatgccgcttctcct
tccgcattcaccaattgctcttccacaacttgctccatcaagcctaccgctttcatttct
taccgtaccctctctcatgtccaccgcggcctcaccatttttcacggctccgcttatgta
cggagctgctccaactcttttcccgaatcctctcctgatgacccctatcggaatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTAACTTGATTGATTCCCGTCGT 255 487 570 Antisense
GCAATTCGGACGAGTCGGCTTCATC 18 323 728 Antisense
GATTGCTCGCTATCTGGAATGCTAT 95 435 757 Antisense
AGAAGTTGAACATGGCTCCGCAGGT 631 73 785 Antisense
TTCGACTTCAGTGCCGGGGGATTTA 642 689 820 Antisense
GATTTATGATTCCTGCAGCGACTTC 649 427 839 Antisense
AATGCAAGCACTAGTCCGGCTGGTT 337 165 868 Antisense
TGAGACAACATCTCCAATGCCGCTT 387 569 900 Antisense
TCAAGCCTACCGCTTTCATTTCTTA 675 625 967 Antisense
GTACGGAGCTGCTCCAACTCTTTTC 526 459 1047 Antisense
CTCCTGATGACCCCTATCGGAATGC 162 235 1081 Antisense
Sequence Source Affymetrix Proprietary Database