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[181957_at]

Description F20B6.4 /REP_DB=WormBase Gene ID /WP=CE04426 /TR=Q19627 /GB=AAA82312.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_high_8_8 > 04      6grp_highest_nonorm > 90      6grp_total > 28      4grp_total > 04      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 404
1 2 3 4 range
A 169.6
(-1.1)
P 		100.2
(-2.7)
P 		84.9
(-3)
M 		348.9
(3)
P 		264
B 218
(-0.6)
P 		119
(-3)
P 		122.4
(-2.9)
P 		361.9
(3)
P 		243
C 181
(-1.2)
P 		92.9
(-2.5)
P 		59.7
(-3)
P 		464
(3)
P 		404
avr 189.5
(-1) 		104
(-2.7) 		89
(-3) 		391.6
(3) 		303

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 156
1 2 3 4 5 6 range
A 237.4
(3)
P 		153.1
(-0.3)
P 		85.5
(-3)
P 		127.3
(-1.3)
P 		107.8
(-2.1)
P 		153.7
(-0.3)
P 		152
B 233.7
(3)
P 		163.3
(-0.7)
P 		151.3
(-1.3)
P 		119.6
(-3)
P 		163.3
(-0.7)
P 		149
(-1.5)
P 		114
C 241.2
(3)
P 		183
(0.5)
P 		99.4
(-3)
P 		168.4
(-0.1)
P 		112.6
(-2.4)
P 		173.8
(0.1)
P 		142
avr 237.4
(3) 		166.5
(-0.4) 		112.1
(-3) 		138.4
(-1.7) 		127.9
(-2.2) 		158.8
(-0.8) 		125
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 61.8
(3)
P 		21.8
(-3)
P 		22.2
(-2.9)
M 		51.1
(1.4)
P 		40
B 69.5
(2.1)
P 		29.4
(-2.9)
P 		28.4
(-3)
P 		77.1
(3)
P 		49
C 59.9
(1.1)
P 		26.7
(-2.4)
P 		20.8
(-3)
P 		78.2
(3)
P 		57
avr 63.7
(2.3) 		26
(-2.7) 		23.8
(-3) 		68.8
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 43.8
(1.2)
P 		54.5
(3)
P 		18.6
(-3)
P 		32.3
(-0.7)
P 		32.5
(-0.7)
P 		37.4
(0.1)
P 		36
B 81.3
(3)
P 		54.4
(-0.3)
P 		36.7
(-2.4)
P 		31.7
(-3)
P 		66.3
(1.2)
P 		55.5
(-0.1)
P 		50
C 89.4
(3)
P 		55.8
(-0.1)
P 		25.3
(-3)
P 		33.9
(-2.2)
P 		36.4
(-2)
P 		58.2
(0.1)
P 		64
avr 71.5
(3) 		54.9
(0.8) 		26.9
(-3) 		32.6
(-2.2) 		45.1
(-0.6) 		50.4
(0.2) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181957_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16695
Sequence Type Exemplar sequence
Representative Public ID F20B6.4 NCBI
Target Description F20B6.4 /REP_DB=WormBase Gene ID /WP=CE04426 /TR=Q19627 /GB=AAA82312.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076311(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076311 NCBI Caenorhabditis elegans F20B6.4 (F20B6.4) mRNA, complete cds. 11/11 None
SNAP00000010534 ENSEMBL cdna:SNAP chromosome:CEL140:X:4194051:4194759:-1 11/11 None
GENEFINDER00000010544 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4194051:4194759:-1 11/11 None
F20B6.4 ENSEMBL cdna:known chromosome:CEL140:X:4194051:4194759:-1 gene:F20B6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4194051-4194760(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F20B6.4
Entrez Gene 184713 Entrez gene
SwissProt Q19627 EMBL-EBI
WormBase CE04426 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA82312 Hypothetical protein F20B6.4 [Caenorhabditis elegans] ref|NP_508712.1| F20B6.4 [Caenorhabditis elegans] 3.0E-94
blast AAA82312 Hypothetical protein F20B6.4 [Caenorhabditis elegans] ref|NP_508712.1| F20B6.4 [Caenorhabditis elegans] 1.0E-90
blast CAE68536 Hypothetical protein CBG14363 [Caenorhabditis briggsae] 2.0E-89
blast CAE68536 Hypothetical protein CBG14363 [Caenorhabditis briggsae] 5.0E-86
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508712.1 1 15-37
F20B6.4 1 15-37
SNAP00000010534 1 15-37
GENEFINDER00000010544 1 15-37
Sequence
Target Sequence 
>C. ELEGANS:181957_AT
ggagacgaccactgaacttgttgacactacggaccattccatttatggcctcgtcgtgtt
tctcgtcatactcatttttttcgcatcactaacgactggtctcattttatgccttctgaa
aatatggtgcacattccacgaacaacgaagaaattctacattcgatcagagaactcgaag
gtcaatcgtcagaagttcgccaaacttcgattatgacagcgacgaggaatcggaaactca
aacaactttgagcaagcgtcgtttctcgcaacgtttaatgagttacgttacaaaaactct
cgataagcgtgaacccccaaaggaaactataaaaccggatattgaacaaccacaagtaca
tgggagaaatggcagtgttagtagccgtcgattaccggcaatcaatgtagagccgacgtc
tgatgttatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGACGACCACTGAACTTGTTGAC 529 519 15 Antisense
TTGTTGACACTACGGACCATTCCAT 365 705 32 Antisense
ACCATTCCATTTATGGCCTCGTCGT 353 85 47 Antisense
GTCATACTCATTTTTTTCGCATCAC 5 469 79 Antisense
ACGACTGGTCTCATTTTATGCCTTC 415 93 106 Antisense
TATGGTGCACATTCCACGAACAACG 570 653 137 Antisense
AGGTCAATCGTCAGAAGTTCGCCAA 507 57 193 Antisense
TTGAGCAAGCGTCGTTTCTCGCAAC 84 707 262 Antisense
TCTCGATAAGCGTGAACCCCCAAAG 527 591 312 Antisense
GCAGTGTTAGTAGCCGTCGATTACC 315 289 386 Antisense
GTAGAGCCGACGTCTGATGTTATGG 480 457 421 Antisense
Sequence Source Affymetrix Proprietary Database